TopicalSciences - Co-ordinated (Double) 0654EnzymesEnzymesPaper 1

Enzymes — Paper 1 · IGCSE Sciences - Co-ordinated (Double) 0654

B5.1· 78 questions · 78 marks · 94 min · 2009–2025· Multiple choice

Every Cambridge IGCSE Sciences - Co-ordinated (Double) Paper 1 question on enzymes, laid out as 26 A4 pages with the mark scheme below. Nothing is left out. Free to read, no account.

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Questions26 pages

Question 1: The diagram shows a piece of Visking tubing (partially permeable) containing starch suspension, held in a beaker of pure water. Saliva, con…Question 2: The diagram shows some parts of the alimentary canal and its associated organs. P Q R S Which organs produce digestive enzymes? A P and Q B…1 / 26
Question 3: Substance X does not react with dilute acid. Substance Y reacts with dilute acid, forming a gas. The graph shows the results of two experim…Question 4: A species of bacterium lives in acidic, hot springs at a temperature of 90 °C. Which conditions will best suit the enzymes of this bacteriu…2 / 26
Question 5: A test-tube containing a starch-amylase mixture is incubated at 35 °C. The graph shows how the amount of starch in the test-tube changes ov…Question 6: A species of bacterium lives in acidic, hot springs at a temperature of 90 °C. Which conditions will best suit the enzymes of this bacteriu…3 / 26
Question 7: The diagram shows part of the digestive system. Where is lipase produced? C D B AQuestion 8: Four statements about enzymes in the human body are listed. 1 They are all proteins. 2 They catalyse reactions in the body. 3 They stop wor…Question 9: What is an enzyme? A a carbohydrate that assists in the digestion of the contents of the stomach B a chemical that absorbs light for photos…4 / 26
Question 10: Which graph shows the effect of temperature on the rate of enzyme activity for a human enzyme? A B rate of rate of enzyme enzyme activity a…Question 11: A student carried out an experiment to investigate the effect of pH on the activity of human amylase. She set up five test-tubes of starch …Question 12: What would be capable of digesting an enzyme? A amylase B bile C lipase D protease5 / 26
Question 13: Eight test-tubes each containing a starch-saliva mixture are set up at four different temperatures. 0 °C 15 °C 35 °C 95 °C For each tempera…Question 14: Which graph shows the effect of pH on the time taken for the breakdown of urea by enzymes? A B time for time for breakdown breakdown of ure…6 / 26
Question 15: Which is an enzyme involved in the digestion of proteins in the stomach? A amylase B bile C lipase D proteaseQuestion 16: One method of preventing food spoilage is to store it at 4 °C in a refrigerator. Why does storing food at low temperatures help to prevent …Question 17: Which statement about all enzymes is correct? A They are used up in the reaction they catalyse. B They speed up reactions. C They work best…Question 18: Which statement about enzymes is correct? A Amylase breaks down fats into fatty acids and glycerol. B Amylase breaks down proteins into ami…Question 19: In some animals, their internal temperature varies with that of their surroundings. Why does the metabolic activity of these animals slow d…7 / 26
Question 20: The diagram shows how the rate of an enzyme-controlled reaction is affected by pH. rate 6 7 8 9 pH What is the optimum pH for this enzyme-c…Question 21: Catalase is an enzyme that breaks down hydrogen peroxide to water and oxygen. In an experiment, the volume of oxygen produced by the break …Question 22: Enzymes are biological catalysts. To which group of compounds do enzymes belong? A carbohydrates B fats C hormones D proteins8 / 26
Question 23: An enzyme from the alimentary canal has an optimum activity at an acidic pH. Which statement is correct? A The enzyme is an amylase and is …Question 24: The graph shows the effect of temperature on the activity of a mammalian enzyme. 14 12 10 activity of enzyme 8 / arbitrary units 6 4 2 0 0 …9 / 26
Question 25: Four test-tubes contain the same volume of starch suspension but each test-tube is kept at a different temperature, as shown. 2 °C 10 °C 30…Question 26: What type of substances are enzymes? A carbohydrates B fats C lipids D proteinsQuestion 27: Some bacteria live in acidic, hot springs. What are the optimum conditions for the enzymes of these bacteria? A 20 °C and pH 4 B 20 °C and …10 / 26
Question 28: Some bacteria live in acidic, hot springs. What are the optimum conditions for the enzymes of these bacteria? A 20 °C and pH 4 B 20 °C and …Question 29: Some bacteria live in acidic, hot springs. What are the optimum conditions for the enzymes of these bacteria? A 20 °C and pH 4 B 20 °C and …Question 30: The graph shows the activity of an enzyme at different temperatures. rate of reaction 0 20 40 60 80 temperature / °C What is the optimum te…11 / 26
Question 31: The graphs show the possible effects of temperature on the rate of reaction of an enzyme. Which graph is correct for a human enzyme? A B ra…Question 32: The graphs show the possible effects of temperature on the rate of reaction of an enzyme. Which graph is correct for a human enzyme? A B ra…12 / 26
Question 33: Which statements are correct for all enzymes? 1 They are proteins. 2 They are unaffected by temperature. 3 They speed up chemical reactions…Question 34: A solution of salivary amylase is boiled in a test tube. The boiled amylase is then added to a solution of starch. Which graph shows what h…13 / 26
Question 35: Which graph shows how enzyme activity is affected by temperature? A B enzyme enzyme activity activity temperature / °C temperature / °C C D…Question 36: Which graph shows the effect of temperature on the activity of a mammalian enzyme? A B rate of rate of reaction reaction 0 60 0 60 temperat…Question 37: Which smaller molecules make up enzymes? A amino acids B fatty acids C glucose D glycerol14 / 26
Question 38: Which type of molecule is an enzyme? A carbohydrate B fat C protein D vitaminQuestion 39: A mixture of starch and saliva was set up at four different temperatures. Each mixture was tested with iodine solution after 15 minutes and…Question 40: A mixture of starch and saliva was set up at four different temperatures. Each mixture was tested with iodine solution after 15 minutes and…15 / 26
Question 41: A mixture of starch and saliva was set up at four different temperatures. Each mixture was tested with iodine solution after 15 minutes and…Question 42: What are biological catalysts? A antibodies B enzymes C hormones D plateletsQuestion 43: What is an enzyme? A a carbohydrate that speeds up the rate of a reaction B a carbohydrate that alters the activity of a target organ C a p…Question 44: Which type of molecule are enzymes? A fat B carbohydrate C protein D DNAQuestion 45: Which type of molecule are enzymes? A fat B carbohydrate C protein D DNA16 / 26
Question 46: A student investigated the effect of pH on an enzyme that digests starch. Which chemical will be needed to determine if any starch has been…Question 47: A student carried out an investigation on the effect of temperature on an enzyme-controlled reaction. The results are shown. axis 2 axis 1 …Question 48: Which graph shows the effect of pH on the rate of activity of an enzyme? A B rate of rate of enzyme enzyme activity activity pH pH C D rate…17 / 26
Question 49: The Pompeii worm lives in deep-sea hydrothermal vents where average temperatures are often as high as 68 C. Which graph represents the act…Question 50: Fungus can live at the base of human hair where the activity of one of its enzymes can cause dry skin. The graph shows the action of the fu…18 / 26
Question 51: Catalase is an enzyme that breaks down hydrogen peroxide to form a foam of water and oxygen. The maximum height of the foam produced at dif…Question 52: Catalase is an enzyme that breaks down hydrogen peroxide to form a foam of water and oxygen. The maximum height of the foam produced at dif…19 / 26
Question 53: Which elements can be found in an enzyme molecule? 1 carbon 2 nitrogen 3 oxygen A 1, 2 and 3 B 1 and 3 only C 1 only D 2 onlyQuestion 54: The rate of a reaction between a powdered metal and a dilute acid is investigated by measuring the volume of hydrogen gas produced per minu…Question 55: Which statement about enzymes is correct? A They are made from fat molecules. B They are used up in a reaction. C They speed up reactions. …Question 56: Which process involves enzymes? A absorption B digestion C egestion D ingestionQuestion 57: The graph shows the rate at which 10 g of starch is broken down by amylase at four different temperatures. 10 8 mass of 6 10 °C starch / g …20 / 26
Question 58: Which type of molecule are enzymes? A amino acids B carbohydrates C fats D proteinsQuestion 59: Which type of molecules speed up chemical digestion? A carbohydrates B enzymes C hormones D fatty acidsQuestion 60: Which type of molecules speed up chemical digestion? A carbohydrates B enzymes C hormones D fatty acidsQuestion 61: Which graph shows the effect of temperature on enzyme activity? A B enzyme enzyme activity activity temperature temperature C D enzyme enzy…21 / 26
Question 62: Which type of molecule are enzymes? A carbohydrate B fat C protein D starchQuestion 63: Which graph is correct? A B rate of rate of enzyme enzyme activity activity pH temperature / °C C D rate of rate of enzyme enzyme activity …Question 64: The graph shows the effect of pH on two different enzymes. enzyme Y enzyme X enzyme activity 0 2 4 6 8 10 12 pH Which statement is correct?…22 / 26
Question 65: Element X increases the rate of some reactions. Element X is unchanged at the end of these reactions. What is the position of element X in …Question 66: Which type of molecules are enzymes? A carbohydrates B fats C oils D proteinsQuestion 67: A scientist places equal volumes of starch and saliva into a test-tube. After 30 minutes, the mixture in the test-tube is tested with iodin…Question 68: A scientist places equal volumes of starch and saliva into a test-tube. After 30 minutes, the mixture in the test-tube is tested with iodin…23 / 26
Question 69: One method of preventing food spoilage is to store it at 4 C in a refrigerator. Why does storing food at low temperatures help to prevent …Question 70: The graph shows the effect of increasing temperature on the rate of an enzyme-controlled reaction. rate of an enzyme- controlled reaction 0…Question 71: The graph shows the effect of increasing temperature on the rate of an enzyme-controlled reaction. rate of an enzyme- controlled reaction 0…24 / 26
Question 72: The graphs show the possible effects of temperature on enzyme activity. Which graph is correct for a human enzyme? A B enzyme enzyme activi…Question 73: Which type of molecules are enzymes? A carbohydrates B fats C hormones D proteinsQuestion 74: Which statements about enzymes are correct? 1 Enzymes are biological catalysts. 2 Enzymes are made of fat. 3 Enzymes are made of protein. A…Question 75: Which statements about enzymes are correct? 1 Enzymes are biological catalysts. 2 Enzymes are made of fat. 3 Enzymes are made of protein. A…25 / 26
Question 76: Which statements are correct for all enzymes? 1 They are proteins. 2 They are not affected by temperature. 3 They increase the rate of chem…Question 77: Some species of bacteria live in alkaline hot springs at temperatures of 80 °C. Which conditions will best suit the enzymes of these bacter…Question 78: Some species of bacteria live in alkaline hot springs at temperatures of 80 °C. Which conditions will best suit the enzymes of these bacter…26 / 26

Mark scheme78 answers

Answers below. Sit the paper first if you are practising.

Pastlit

Sciences - Co-ordinated (Double) 0654 · Enzymes — Paper 1

IGCSE · topical answer key — answer key (teacher use)

Question

Answer

Marks

1C1
2B1
3B1
4C1
5B1
6C1
7A1
8A1
9D1
10B1
11A1
12D1
13A1
14D1
15D1
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17B1
18C1
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20C1
21D1
22D1
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24B1
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26D1
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31A1
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35D1
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42B1
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44C1
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46A1
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48D1
49B1
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Pastlit

Sciences - Co-ordinated (Double) 0654 · Enzymes — Paper 1

IGCSE · topical answer key — answer key (teacher use)

Question

Answer

Marks

50B1
51C1
52C1
53A1
54B1
55C1
56B1
57C1
58D1
59B1
60B1
61D1
62C1
63B1
64B1
65D1
66D1
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Another paper, or another topic

All of Enzymes

Questions as text

Q1 · The diagram shows a piece of Visking tubing (partially permeable) containing starch… 0654/11 May/June 2009

6 The diagram shows a piece of Visking tubing (partially permeable) containing starch suspension, held in a beaker of pure water. Saliva, containing the enzyme amylase, is added to the starch and left for two hours. saliva Visking tubing starch suspension sugar now pure present water 10.00 am 12 noon What does the experiment show? A Amylase is a solvent for starch. B Saliva passes through the Visking tubing. C Starch can be changed to sugar. D Starch is soluble in pure water.

1 marks

Answer: C

This question in 0654/11 May/June 2009

Q2 · The diagram shows some parts of the alimentary canal and its associated organs 0654/12 Oct/Nov 2011

8 The diagram shows some parts of the alimentary canal and its associated organs. P Q R S Which organs produce digestive enzymes? A P and Q B Q and R C R and S D S and P

1 marks

Answer: B

This question in 0654/12 Oct/Nov 2011

Q3 · Substance X does not react with dilute acid 0654/13 Oct/Nov 2013

20 Substance X does not react with dilute acid. Substance Y reacts with dilute acid, forming a gas. The graph shows the results of two experiments. experiment 1 Y + dilute acid experiment 2 X + Y + dilute acid experiment 2 total volume of gaseous product experiment 1 0 time What do these results show? X is a catalyst X is quickly used up A ✓ ✓ key B ✓ ✗ ✓= true C ✗ ✓ ✗= false D ✗ ✗

1 marks

Answer: B

This question in 0654/13 Oct/Nov 2013

Q4 · A species of bacterium lives in acidic, hot springs at a temperature of 90 °C 0654/11 May/June 2014

4 A species of bacterium lives in acidic, hot springs at a temperature of 90 °C. Which conditions will best suit the enzymes of this bacterium? A 30 °C and pH 4 B 30 °C and pH 9 C 80 °C and pH 4 D 80 °C and pH 9

1 marks

Answer: C

This question in 0654/11 May/June 2014

Q5 · A test-tube containing a starch-amylase mixture is incubated at 35 °C 0654/12 May/June 2014

4 A test-tube containing a starch-amylase mixture is incubated at 35 °C. The graph shows how the amount of starch in the test-tube changes over the next five minutes. amount of starch at the start amount of starch remaining 0 1 2 3 4 5 time / minutes Which graph shows what happens if a similar starch-amylase mixture is incubated at 100 °C? A B amount amount of starch of starch remaining remaining 0 1 2 3 4 5 0 1 2 3 4 5 time / minutes time / minutes C D amount amount of starch of starch remaining remaining 0 1 2 3 4 5 0 1 2 3 4 5 time / minutes time / minutes

1 marks

Answer: B

This question in 0654/12 May/June 2014

Q6 · A species of bacterium lives in acidic, hot springs at a temperature of 90 °C 0654/13 May/June 2014

3 A species of bacterium lives in acidic, hot springs at a temperature of 90 °C. Which conditions will best suit the enzymes of this bacterium? A 30 °C and pH 4 B 30 °C and pH 9 C 80 °C and pH 4 D 80 °C and pH 9

1 marks

Answer: C

This question in 0654/13 May/June 2014

Q7 · The diagram shows part of the digestive system 0654/11 Oct/Nov 2014

3 The diagram shows part of the digestive system. Where is lipase produced? C D B A

1 marks

Answer: A

This question in 0654/11 Oct/Nov 2014

Q8 · Four statements about enzymes in the human body are listed 0654/13 Oct/Nov 2014

3 Four statements about enzymes in the human body are listed. 1 They are all proteins. 2 They catalyse reactions in the body. 3 They stop working at temperatures over 75 °C. 4 They work faster at 30 °C than at 10 °C. Which statements are correct? A 1, 2, 3 and 4 B 1 and 3 only C 2 and 3 only D 2 and 4 only

1 marks

Answer: A

This question in 0654/13 Oct/Nov 2014

Q9 · What is an enzyme? 0654/11 May/June 2015

4 What is an enzyme? A a carbohydrate that assists in the digestion of the contents of the stomach B a chemical that absorbs light for photosynthesis C a chemical that alters the activity of a target organ D a protein that alters the rate of a chemical reaction

1 marks

Answer: D

This question in 0654/11 May/June 2015

Q10 · Which graph shows the effect of temperature on the rate of enzyme activity for a human… 0654/12 May/June 2015

3 Which graph shows the effect of temperature on the rate of enzyme activity for a human enzyme? A B rate of rate of enzyme enzyme activity activity 0 10 20 30 40 50 60 0 10 20 30 40 50 60 temperature / °C temperature / °C C D rate of rate of enzyme enzyme activity activity 0 10 20 30 40 50 60 0 10 20 30 40 50 60 temperature / °C temperature / °C

1 marks

Answer: B

This question in 0654/12 May/June 2015

Q11 · A student carried out an experiment to investigate the effect of pH on the activity of… 0654/13 May/June 2015

4 A student carried out an experiment to investigate the effect of pH on the activity of human amylase. She set up five test-tubes of starch and amylase mixture, each at a different pH. starch and amylase mixture pH 3 pH 5 pH 7 pH 9 pH 11 At which temperature(s) should the test-tubes be kept during this experiment? A all at 37 °C B all at 100 °C C at a range of temperatures between 0 °C and 50 °C D at a range of temperatures between 20 °C and 70 °C

1 marks

Answer: A

This question in 0654/13 May/June 2015

Q12 · What would be capable of digesting an enzyme? 0654/11 Oct/Nov 2015

4 What would be capable of digesting an enzyme? A amylase B bile C lipase D protease

1 marks

Answer: D

This question in 0654/11 Oct/Nov 2015

Q13 · Eight test-tubes each containing a starch-saliva mixture are set up at four different… 0654/12 Oct/Nov 2015

4 Eight test-tubes each containing a starch-saliva mixture are set up at four different temperatures. 0 °C 15 °C 35 °C 95 °C For each temperature, one test-tube is tested with iodine solution after 15 minutes, and the other after 30 minutes. The results are shown in the table. temperature colour with iodine solution / °C 15 minutes 30 minutes 0 blue-black blue-black 15 blue-black brown 35 brown brown 95 blue-black blue-black What do the results suggest? A The enzyme in saliva is inactive at 95 °C. B The enzyme in saliva is slow to work at 35 °C. C The enzyme in saliva works equally well at 15 °C and 35 °C. D The enzyme in saliva works faster at higher temperatures.

1 marks

Answer: A

This question in 0654/12 Oct/Nov 2015

Q14 · Which graph shows the effect of pH on the time taken for the breakdown of urea by enzymes? 0654/13 Oct/Nov 2015

3 Which graph shows the effect of pH on the time taken for the breakdown of urea by enzymes? A B time for time for breakdown breakdown of urea of urea 00 00 pH pH C D time for time for breakdown breakdown of urea of urea 00 00 pH pH

1 marks

Answer: D

This question in 0654/13 Oct/Nov 2015

Q15 · Which is an enzyme involved in the digestion of proteins in the stomach? 0654/11 May/June 2016

2 Which is an enzyme involved in the digestion of proteins in the stomach? A amylase B bile C lipase D protease

1 marks

Answer: D

This question in 0654/11 May/June 2016

Q16 · One method of preventing food spoilage is to store it at 4 °C in a refrigerator 0654/12 May/June 2016

3 One method of preventing food spoilage is to store it at 4 °C in a refrigerator. Why does storing food at low temperatures help to prevent food spoilage? A It decreases enzyme activity. B It denatures enzymes. C It increases enzyme production. D It kills cells.

1 marks

Answer: A

This question in 0654/12 May/June 2016

Q17 · Which statement about all enzymes is correct? 0654/13 May/June 2016

3 Which statement about all enzymes is correct? A They are used up in the reaction they catalyse. B They speed up reactions. C They work best above 40 °C. D They work best at a pH of 7.0.

1 marks

Answer: B

This question in 0654/13 May/June 2016

Q18 · Which statement about enzymes is correct? 0654/11 Oct/Nov 2016

2 Which statement about enzymes is correct? A Amylase breaks down fats into fatty acids and glycerol. B Amylase breaks down proteins into amino acids. C Lipase breaks down fats into fatty acids and glycerol. D Lipase breaks down proteins into amino acids.

1 marks

Answer: C

This question in 0654/11 Oct/Nov 2016

Q19 · In some animals, their internal temperature varies with that of their surroundings 0654/12 Oct/Nov 2016

2 In some animals, their internal temperature varies with that of their surroundings. Why does the metabolic activity of these animals slow down when it is cold? A Enzyme activity is slow. B Heart rate drops. C The blood freezes. D The absorption of food is slow.

1 marks

Answer: A

This question in 0654/12 Oct/Nov 2016

Q20 · The diagram shows how the rate of an enzyme-controlled reaction is affected by pH 0654/13 Oct/Nov 2016

2 The diagram shows how the rate of an enzyme-controlled reaction is affected by pH. rate 6 7 8 9 pH What is the optimum pH for this enzyme-controlled reaction? A 6 B 6.5 C 7.5 D 9

1 marks

Answer: C

This question in 0654/13 Oct/Nov 2016

Q21 · Catalase is an enzyme that breaks down hydrogen peroxide to water and oxygen 0654/11 May/June 2017

3 Catalase is an enzyme that breaks down hydrogen peroxide to water and oxygen. In an experiment, the volume of oxygen produced by the break down of hydrogen peroxide was measured. The graph shows the results. volume of oxygen produced time T Which description is the rate of oxygen production at time T? A at its maximum B steadily decreasing C steadily increasing D zero

1 marks

Answer: D

This question in 0654/11 May/June 2017

Q22 · Enzymes are biological catalysts 0654/12 May/June 2017

5 Enzymes are biological catalysts. To which group of compounds do enzymes belong? A carbohydrates B fats C hormones D proteins

1 marks

Answer: D

This question in 0654/12 May/June 2017

Q23 · An enzyme from the alimentary canal has an optimum activity at an acidic pH 0654/13 May/June 2017

3 An enzyme from the alimentary canal has an optimum activity at an acidic pH. Which statement is correct? A The enzyme is an amylase and is found in the mouth. B The enzyme is a protease and is found in the mouth. C The enzyme is an amylase and is found in the stomach. D The enzyme is a protease and is found in the stomach.

1 marks

Answer: D

This question in 0654/13 May/June 2017

Q24 · The graph shows the effect of temperature on the activity of a mammalian enzyme 0654/11 Oct/Nov 2017

9 The graph shows the effect of temperature on the activity of a mammalian enzyme. 14 12 10 activity of enzyme 8 / arbitrary units 6 4 2 0 0 10 20 30 40 50 60 70 temperature / °C Which conclusion can be drawn from the graph? A The activity increases in a linear manner up to 35 °C. B The activity is four times greater at 40 °C than at 20 °C. C The enzyme has a higher activity at 60 °C than at 0 °C. D The optimum temperature for this enzyme is 37 °C.

1 marks

Answer: B

This question in 0654/11 Oct/Nov 2017

Q25 · Four test-tubes contain the same volume of starch suspension but each test-tube is kept… 0654/12 Oct/Nov 2017

2 Four test-tubes contain the same volume of starch suspension but each test-tube is kept at a different temperature, as shown. 2 °C 10 °C 30 °C 60 °C An equal quantity of enzyme is added to each test-tube and, after ten minutes, iodine solution is added to each test-tube. The results are shown below. 2 °C 10 °C 30 °C 60 °C What do the results show? A At 2 °C the enzyme works slowly. B At 10 °C the enzyme does not work. C At 30 °C the enzyme works well. D At 60 °C the enzyme works best.

1 marks

Answer: C

This question in 0654/12 Oct/Nov 2017

Q26 · What type of substances are enzymes? 0654/13 Oct/Nov 2017

1 What type of substances are enzymes? A carbohydrates B fats C lipids D proteins

1 marks

Answer: D

This question in 0654/13 Oct/Nov 2017

Q27 · Some bacteria live in acidic, hot springs 0654/11 Oct/Nov 2018

3 Some bacteria live in acidic, hot springs. What are the optimum conditions for the enzymes of these bacteria? A 20 °C and pH 4 B 20 °C and pH 9 C 80 °C and pH 4 D 80 °C and pH 9

1 marks

Answer: C

This question in 0654/11 Oct/Nov 2018

Q28 · Some bacteria live in acidic, hot springs 0654/12 Oct/Nov 2018

2 Some bacteria live in acidic, hot springs. What are the optimum conditions for the enzymes of these bacteria? A 20 °C and pH 4 B 20 °C and pH 9 C 80 °C and pH 4 D 80 °C and pH 9

1 marks

Answer: C

This question in 0654/12 Oct/Nov 2018

Q29 · Some bacteria live in acidic, hot springs 0654/13 Oct/Nov 2018

3 Some bacteria live in acidic, hot springs. What are the optimum conditions for the enzymes of these bacteria? A 20 °C and pH 4 B 20 °C and pH 9 C 80 °C and pH 4 D 80 °C and pH 9

1 marks

Answer: C

This question in 0654/13 Oct/Nov 2018

Q30 · The graph shows the activity of an enzyme at different temperatures 0654/11 May/June 2019

4 The graph shows the activity of an enzyme at different temperatures. rate of reaction 0 20 40 60 80 temperature / °C What is the optimum temperature for this enzyme? A 20 °C B 40 °C C 60 °C D 80 °C

1 marks

Answer: C

This question in 0654/11 May/June 2019

Q31 · The graphs show the possible effects of temperature on the rate of reaction of an enzyme 0654/12 May/June 2019

4 The graphs show the possible effects of temperature on the rate of reaction of an enzyme. Which graph is correct for a human enzyme? A B rate of rate of reaction reaction 0 30 60 0 30 60 temperature / °C temperature / °C C D rate of rate of reaction reaction 0 30 60 0 30 60 temperature / °C temperature / °C

1 marks

Answer: A

This question in 0654/12 May/June 2019

Q32 · The graphs show the possible effects of temperature on the rate of reaction of an enzyme 0654/13 May/June 2019

4 The graphs show the possible effects of temperature on the rate of reaction of an enzyme. Which graph is correct for a human enzyme? A B rate of rate of reaction reaction 0 30 60 0 30 60 temperature / °C temperature / °C C D rate of rate of reaction reaction 0 30 60 0 30 60 temperature / °C temperature / °C

1 marks

Answer: A

This question in 0654/13 May/June 2019

Q33 · Which statements are correct for all enzymes? 0654/11 Oct/Nov 2019

4 Which statements are correct for all enzymes? 1 They are proteins. 2 They are unaffected by temperature. 3 They speed up chemical reactions. 4 They work best at a high pH. A 1, 2 and 4 B 1, 3 and 4 C 1 and 3 only D 2 and 4 only

1 marks

Answer: C

This question in 0654/11 Oct/Nov 2019

Q34 · A solution of salivary amylase is boiled in a test tube 0654/12 Oct/Nov 2019

4 A solution of salivary amylase is boiled in a test tube. The boiled amylase is then added to a solution of starch. Which graph shows what happens to the concentration of starch in the mixture during the next 10 minutes? A B concentration concentration of starch of starch 0 2 4 6 8 10 0 2 4 6 8 10 time / minutes time / minutes C D concentration concentration of starch of starch 0 2 4 6 8 10 0 2 4 6 8 10 time / minutes time / minutes

1 marks

Answer: B

This question in 0654/12 Oct/Nov 2019

Q35 · Which graph shows how enzyme activity is affected by temperature? 0654/13 Oct/Nov 2019

4 Which graph shows how enzyme activity is affected by temperature? A B enzyme enzyme activity activity temperature / °C temperature / °C C D enzyme enzyme activity activity temperature / °C temperature / °C

1 marks

Answer: D

This question in 0654/13 Oct/Nov 2019

Q36 · Which graph shows the effect of temperature on the activity of a mammalian enzyme? 0654/11 May/June 2020

4 Which graph shows the effect of temperature on the activity of a mammalian enzyme? A B rate of rate of reaction reaction 0 60 0 60 temperature / °C temperature / °C C D rate of rate of reaction reaction 0 60 0 60 temperature / °C temperature / °C

1 marks

Answer: C

This question in 0654/11 May/June 2020

Q37 · Which smaller molecules make up enzymes? 0654/12 May/June 2020

4 Which smaller molecules make up enzymes? A amino acids B fatty acids C glucose D glycerol

1 marks

Answer: A

This question in 0654/12 May/June 2020

Q38 · Which type of molecule is an enzyme? 0654/13 May/June 2020

4 Which type of molecule is an enzyme? A carbohydrate B fat C protein D vitamin

1 marks

Answer: C

This question in 0654/13 May/June 2020

Q39 · A mixture of starch and saliva was set up at four different temperatures 0654/11 Oct/Nov 2020

4 A mixture of starch and saliva was set up at four different temperatures. Each mixture was tested with iodine solution after 15 minutes and again after 30 minutes. The results are shown in the table. temperature colour with iodine solution / C 15 minutes 30 minutes 0 blue-black blue-black 15 blue-black brown 35 brown brown 95 blue-black blue-black What do the results suggest? A The enzyme in saliva is inactive at 95 C. B The enzyme in saliva is slow to work at 35 C. C The enzyme in saliva works equally well at 15 C and 35 C. D The enzyme in saliva works faster at higher temperatures.

1 marks

Answer: A

This question in 0654/11 Oct/Nov 2020

Q40 · A mixture of starch and saliva was set up at four different temperatures 0654/12 Oct/Nov 2020

4 A mixture of starch and saliva was set up at four different temperatures. Each mixture was tested with iodine solution after 15 minutes and again after 30 minutes. The results are shown in the table. temperature colour with iodine solution / C 15 minutes 30 minutes 0 blue-black blue-black 15 blue-black brown 35 brown brown 95 blue-black blue-black What do the results suggest? A The enzyme in saliva is inactive at 95 C. B The enzyme in saliva is slow to work at 35 C. C The enzyme in saliva works equally well at 15 C and 35 C. D The enzyme in saliva works faster at higher temperatures.

1 marks

Answer: A

This question in 0654/12 Oct/Nov 2020

Q41 · A mixture of starch and saliva was set up at four different temperatures 0654/13 Oct/Nov 2020

4 A mixture of starch and saliva was set up at four different temperatures. Each mixture was tested with iodine solution after 15 minutes and again after 30 minutes. The results are shown in the table. temperature colour with iodine solution / C 15 minutes 30 minutes 0 blue-black blue-black 15 blue-black brown 35 brown brown 95 blue-black blue-black What do the results suggest? A The enzyme in saliva is inactive at 95 C. B The enzyme in saliva is slow to work at 35 C. C The enzyme in saliva works equally well at 15 C and 35 C. D The enzyme in saliva works faster at higher temperatures.

1 marks

Answer: A

This question in 0654/13 Oct/Nov 2020

Q42 · What are biological catalysts? 0654/12 Feb/March 2021

4 What are biological catalysts? A antibodies B enzymes C hormones D platelets

1 marks

Answer: B

This question in 0654/12 Feb/March 2021

Q43 · What is an enzyme? 0654/11 May/June 2021

4 What is an enzyme? A a carbohydrate that speeds up the rate of a reaction B a carbohydrate that alters the activity of a target organ C a protein that alters the activity of a target organ D a protein that speeds up the rate of a reaction

1 marks

Answer: D

This question in 0654/11 May/June 2021

Q44 · Which type of molecule are enzymes? 0654/12 May/June 2021

4 Which type of molecule are enzymes? A fat B carbohydrate C protein D DNA

1 marks

Answer: C

This question in 0654/12 May/June 2021

Q45 · Which type of molecule are enzymes? 0654/13 May/June 2021

4 Which type of molecule are enzymes? A fat B carbohydrate C protein D DNA

1 marks

Answer: C

This question in 0654/13 May/June 2021

Q46 · A student investigated the effect of pH on an enzyme that digests starch 0654/11 Oct/Nov 2021

4 A student investigated the effect of pH on an enzyme that digests starch. Which chemical will be needed to determine if any starch has been digested? A Benedict’s solution B biuret solution C iodine solution D ethanol

1 marks

Answer: A

This question in 0654/11 Oct/Nov 2021

Q47 · A student carried out an investigation on the effect of temperature on an… 0654/12 Oct/Nov 2021

4 A student carried out an investigation on the effect of temperature on an enzyme-controlled reaction. The results are shown. axis 2 axis 1 Which labels does the student need to add for the axes labelled axis 1 and axis 2? axis 1 axis 2 A rate of reaction temperature B rate of reaction time C temperature rate of reaction D time rate of reaction

1 marks

Answer: C

This question in 0654/12 Oct/Nov 2021

Q48 · Which graph shows the effect of pH on the rate of activity of an enzyme? 0654/13 Oct/Nov 2021

4 Which graph shows the effect of pH on the rate of activity of an enzyme? A B rate of rate of enzyme enzyme activity activity pH pH C D rate of rate of enzyme enzyme activity activity pH pH

1 marks

Answer: D

This question in 0654/13 Oct/Nov 2021

Q49 · The Pompeii worm lives in deep-sea hydrothermal vents where average temperatures are… 0654/12 Feb/March 2022

4 The Pompeii worm lives in deep-sea hydrothermal vents where average temperatures are often as high as 68 C. Which graph represents the activity of enzymes found in the Pompeii worm? A B rate of rate of reaction reaction 0 10 20 30 40 50 60 70 80 90 0 10 20 30 40 50 60 70 80 90 temperature / °C temperature / °C C D rate of rate of reaction reaction 0 10 20 30 40 50 60 70 80 90 0 10 20 30 40 50 60 70 80 90 temperature / °C temperature / °C

1 marks

Answer: B

This question in 0654/12 Feb/March 2022

Q50 · Fungus can live at the base of human hair where the activity of one of its enzymes can… 0654/11 May/June 2022

4 Fungus can live at the base of human hair where the activity of one of its enzymes can cause dry skin. The graph shows the action of the fungal enzyme at different pH values. rate of reaction 0 1 2 3 4 5 6 7 8 9 pH Different shampoos have different pH values. Which shampoo causes the greatest amount of dry skin? A pH 3 B pH 5 C pH 7 D pH 9

1 marks

Answer: B

This question in 0654/11 May/June 2022

Q51 · Catalase is an enzyme that breaks down hydrogen peroxide to form a foam of water and… 0654/12 May/June 2022

4 Catalase is an enzyme that breaks down hydrogen peroxide to form a foam of water and oxygen. The maximum height of the foam produced at different temperatures in a given length of time is measured. The rate of the reaction is proportional to the height of foam. The table shows the results. temperature / C height of foam / mm 10 1.2 20 3.1 30 4.2 40 5.4 50 3.6 60 0.0 Which conclusion can be drawn from these results? A Increasing the temperature decreases the rate of the reaction up to 50 C. B Increasing the temperature decreases the rate of the reaction up to 60 C. C Increasing the temperature increases the rate of the reaction up to 40 C. D Increasing the temperature increases the rate of the reaction up to 50 C.

1 marks

Answer: C

This question in 0654/12 May/June 2022

Q52 · Catalase is an enzyme that breaks down hydrogen peroxide to form a foam of water and… 0654/13 May/June 2022

4 Catalase is an enzyme that breaks down hydrogen peroxide to form a foam of water and oxygen. The maximum height of the foam produced at different temperatures in a given length of time is measured. The rate of the reaction is proportional to the height of foam. The table shows the results. temperature / C height of foam / mm 10 1.2 20 3.1 30 4.2 40 5.4 50 3.6 60 0.0 Which conclusion can be drawn from these results? A Increasing the temperature decreases the rate of the reaction up to 50 C. B Increasing the temperature decreases the rate of the reaction up to 60 C. C Increasing the temperature increases the rate of the reaction up to 40 C. D Increasing the temperature increases the rate of the reaction up to 50 C.

1 marks

Answer: C

This question in 0654/13 May/June 2022

Q53 · Which elements can be found in an enzyme molecule? 0654/11 Oct/Nov 2022

4 Which elements can be found in an enzyme molecule? 1 carbon 2 nitrogen 3 oxygen A 1, 2 and 3 B 1 and 3 only C 1 only D 2 only

1 marks

Answer: A

This question in 0654/11 Oct/Nov 2022

Q54 · The rate of a reaction between a powdered metal and a dilute acid is investigated by… 0654/11 Oct/Nov 2022

19 The rate of a reaction between a powdered metal and a dilute acid is investigated by measuring the volume of hydrogen gas produced per minute. The investigation is repeated using a catalyst. The same mass of powdered metal and the same volume and concentration of acid is used. Which statement about the second investigation is correct? A The rate of the reaction is higher and a greater total volume of hydrogen is produced. B The rate of the reaction is higher and the same total volume of hydrogen is produced. C The rate of the reaction is lower and a smaller total volume of hydrogen is produced. D The rate of the reaction is lower and the same total volume of hydrogen is produced.

1 marks

Answer: B

This question in 0654/11 Oct/Nov 2022

Q55 · Which statement about enzymes is correct? 0654/12 Oct/Nov 2022

4 Which statement about enzymes is correct? A They are made from fat molecules. B They are used up in a reaction. C They speed up reactions. D They are not affected by temperature.

1 marks

Answer: C

This question in 0654/12 Oct/Nov 2022

Q56 · Which process involves enzymes? 0654/13 Oct/Nov 2022

4 Which process involves enzymes? A absorption B digestion C egestion D ingestion

1 marks

Answer: B

This question in 0654/13 Oct/Nov 2022

Q57 · The graph shows the rate at which 10 g of starch is broken down by amylase at four… 0654/12 Feb/March 2023

4 The graph shows the rate at which 10 g of starch is broken down by amylase at four different temperatures. 10 8 mass of 6 10 °C starch / g 4 25 °C 70 °C 2 40 °C 0 time At which temperature does amylase work best to break down starch? A 10 C B 25 C C 40 C D 70 C

1 marks

Answer: C

This question in 0654/12 Feb/March 2023

Q58 · Which type of molecule are enzymes? 0654/11 May/June 2023

4 Which type of molecule are enzymes? A amino acids B carbohydrates C fats D proteins

1 marks

Answer: D

This question in 0654/11 May/June 2023

Q59 · Which type of molecules speed up chemical digestion? 0654/12 May/June 2023

4 Which type of molecules speed up chemical digestion? A carbohydrates B enzymes C hormones D fatty acids

1 marks

Answer: B

This question in 0654/12 May/June 2023

Q60 · Which type of molecules speed up chemical digestion? 0654/13 May/June 2023

4 Which type of molecules speed up chemical digestion? A carbohydrates B enzymes C hormones D fatty acids

1 marks

Answer: B

This question in 0654/13 May/June 2023

Q61 · Which graph shows the effect of temperature on enzyme activity? 0654/11 Oct/Nov 2023

4 Which graph shows the effect of temperature on enzyme activity? A B enzyme enzyme activity activity temperature temperature C D enzyme enzyme activity activity temperature temperature

1 marks

Answer: D

This question in 0654/11 Oct/Nov 2023

Q62 · Which type of molecule are enzymes? 0654/12 Oct/Nov 2023

4 Which type of molecule are enzymes? A carbohydrate B fat C protein D starch

1 marks

Answer: C

This question in 0654/12 Oct/Nov 2023

Q63 · Which graph is correct? 0654/13 Oct/Nov 2023

4 Which graph is correct? A B rate of rate of enzyme enzyme activity activity pH temperature / °C C D rate of rate of enzyme enzyme activity activity pH temperature / °C

1 marks

Answer: B

This question in 0654/13 Oct/Nov 2023

Q64 · The graph shows the effect of pH on two different enzymes 0654/12 Feb/March 2024

4 The graph shows the effect of pH on two different enzymes. enzyme Y enzyme X enzyme activity 0 2 4 6 8 10 12 pH Which statement is correct? A Both enzymes show no activity in conditions of pH 4. B Both enzymes are active in conditions of pH 5. C Only enzyme X is active in conditions of pH 10. D Only enzyme Y is active in conditions of pH 3.

1 marks

Answer: B

This question in 0654/12 Feb/March 2024

Q65 · Element X increases the rate of some reactions 0654/12 Feb/March 2024

23 Element X increases the rate of some reactions. Element X is unchanged at the end of these reactions. What is the position of element X in the Periodic Table? I II III IV V VI VII 0 B C A D

1 marks

Answer: D

This question in 0654/12 Feb/March 2024

Q66 · Which type of molecules are enzymes? 0654/11 May/June 2024

4 Which type of molecules are enzymes? A carbohydrates B fats C oils D proteins

1 marks

Answer: D

This question in 0654/11 May/June 2024

Q67 · A scientist places equal volumes of starch and saliva into a test-tube 0654/12 May/June 2024

6 A scientist places equal volumes of starch and saliva into a test-tube. After 30 minutes, the mixture in the test-tube is tested with iodine solution. The iodine solution remains brown. Which process does this experiment demonstrate? A absorption B assimilation C digestion D ingestion

1 marks

Answer: C

This question in 0654/12 May/June 2024

Q68 · A scientist places equal volumes of starch and saliva into a test-tube 0654/13 May/June 2024

6 A scientist places equal volumes of starch and saliva into a test-tube. After 30 minutes, the mixture in the test-tube is tested with iodine solution. The iodine solution remains brown. Which process does this experiment demonstrate? A absorption B assimilation C digestion D ingestion

1 marks

Answer: C

This question in 0654/13 May/June 2024

Q69 · One method of preventing food spoilage is to store it at 4 C in a refrigerator 0654/11 Oct/Nov 2024

4 One method of preventing food spoilage is to store it at 4 C in a refrigerator. Why does storing food at low temperatures help to prevent food spoilage? A It decreases enzyme activity. B It denatures enzymes. C It increases enzyme production. D It kills cells.

1 marks

Answer: A

This question in 0654/11 Oct/Nov 2024

Q70 · The graph shows the effect of increasing temperature on the rate of an enzyme-controlled… 0654/12 Oct/Nov 2024

4 The graph shows the effect of increasing temperature on the rate of an enzyme-controlled reaction. rate of an enzyme- controlled reaction 0 10 20 30 40 50 60 70 temperature / °C Which statement describes what is happening between 10 C and 30 C on the graph? A An increase in the rate of reaction increases the temperature of the reaction. B An increase in temperature has no effect on the rate of a reaction. C As the rate of reaction increases, the temperature has no effect. D As the temperature increases, the rate of reaction also increases.

1 marks

Answer: D

This question in 0654/12 Oct/Nov 2024

Q71 · The graph shows the effect of increasing temperature on the rate of an enzyme-controlled… 0654/13 Oct/Nov 2024

4 The graph shows the effect of increasing temperature on the rate of an enzyme-controlled reaction. rate of an enzyme- controlled reaction 0 10 20 30 40 50 60 70 temperature / °C Which statement describes what is happening between 10 C and 30 C on the graph? A An increase in the rate of reaction increases the temperature of the reaction. B An increase in temperature has no effect on the rate of a reaction. C As the rate of reaction increases, the temperature has no effect. D As the temperature increases, the rate of reaction also increases.

1 marks

Answer: D

This question in 0654/13 Oct/Nov 2024

Q72 · The graphs show the possible effects of temperature on enzyme activity 0654/12 Feb/March 2025

6 The graphs show the possible effects of temperature on enzyme activity. Which graph is correct for a human enzyme? A B enzyme enzyme activity activity 0 30 60 0 30 60 temperature / °C temperature / °C C D enzyme enzyme activity activity 0 30 60 0 30 60 temperature / °C temperature / °C

1 marks

Answer: A

This question in 0654/12 Feb/March 2025

Q73 · Which type of molecules are enzymes? 0654/11 May/June 2025

5 Which type of molecules are enzymes? A carbohydrates B fats C hormones D proteins

1 marks

Answer: D

This question in 0654/11 May/June 2025

Q74 · Which statements about enzymes are correct? 0654/12 May/June 2025

4 Which statements about enzymes are correct? 1 Enzymes are biological catalysts. 2 Enzymes are made of fat. 3 Enzymes are made of protein. A 1 and 2 B 1 and 3 C 1 only D 3 only

1 marks

Answer: B

This question in 0654/12 May/June 2025

Q75 · Which statements about enzymes are correct? 0654/13 May/June 2025

4 Which statements about enzymes are correct? 1 Enzymes are biological catalysts. 2 Enzymes are made of fat. 3 Enzymes are made of protein. A 1 and 2 B 1 and 3 C 1 only D 3 only

1 marks

Answer: B

This question in 0654/13 May/June 2025

Q76 · Which statements are correct for all enzymes? 0654/11 Oct/Nov 2025

5 Which statements are correct for all enzymes? 1 They are proteins. 2 They are not affected by temperature. 3 They increase the rate of chemical reactions. 4 They work best at a high pH. A 1, 2 and 4 B 1, 3 and 4 C 1 and 3 only D 2 and 4 only

1 marks

Answer: C

This question in 0654/11 Oct/Nov 2025

Q77 · Some species of bacteria live in alkaline hot springs at temperatures of 80 °C 0654/12 Oct/Nov 2025

3 Some species of bacteria live in alkaline hot springs at temperatures of 80 °C. Which conditions will best suit the enzymes of these bacteria? temperature / °C pH A 30 3 B 30 9 C 70 3 D 70 9

1 marks

Answer: D

This question in 0654/12 Oct/Nov 2025

Q78 · Some species of bacteria live in alkaline hot springs at temperatures of 80 °C 0654/13 Oct/Nov 2025

3 Some species of bacteria live in alkaline hot springs at temperatures of 80 °C. Which conditions will best suit the enzymes of these bacteria? temperature / °C pH A 30 3 B 30 9 C 70 3 D 70 9

1 marks

Answer: D

This question in 0654/13 Oct/Nov 2025