B4.1· 84 questions · 84 marks · 101 min · 2006–2025· Multiple choice
Every Cambridge IGCSE Sciences - Co-ordinated (Double) Paper 1 question on biological molecules, laid out as 18 A4 pages with the mark scheme below. Nothing is left out. Free to read, no account.


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18 / 18Answers below. Sit the paper first if you are practising.
Pastlit
Sciences - Co-ordinated (Double) 0654 · Biological molecules — Paper 1
IGCSE · topical answer key — answer key (teacher use)
Question
Answer
Marks
Pastlit
Sciences - Co-ordinated (Double) 0654 · Biological molecules — Paper 1
IGCSE · topical answer key — answer key (teacher use)
Question
Answer
Marks
| Question | Answer | Marks | From |
|---|---|---|---|
| 1 | D | 1 | 0654/11 May/June 2006 |
| 2 | C | 1 | 0654/11 Oct/Nov 2007 |
| 3 | C | 1 | 0654/11 Oct/Nov 2007 |
| 4 | B | 1 | 0654/11 Oct/Nov 2008 |
| 5 | A | 1 | 0654/11 May/June 2009 |
| 6 | A | 1 | 0654/12 Oct/Nov 2010 |
| 7 | D | 1 | 0654/13 Oct/Nov 2010 |
| 8 | A | 1 | 0654/13 Oct/Nov 2010 |
| 9 | B | 1 | 0654/11 May/June 2011 |
| 10 | C | 1 | 0654/11 May/June 2011 |
| 11 | B | 1 | 0654/12 May/June 2011 |
| 12 | C | 1 | 0654/12 May/June 2011 |
| 13 | B | 1 | 0654/13 May/June 2011 |
| 14 | C | 1 | 0654/13 May/June 2011 |
| 15 | B | 1 | 0654/11 Oct/Nov 2011 |
| 16 | B | 1 | 0654/13 Oct/Nov 2011 |
| 17 | B | 1 | 0654/11 May/June 2013 |
| 18 | B | 1 | 0654/12 May/June 2013 |
| 19 | B | 1 | 0654/13 May/June 2013 |
| 20 | B | 1 | 0654/11 Oct/Nov 2013 |
| 21 | B | 1 | 0654/12 Oct/Nov 2013 |
| 22 | C | 1 | 0654/12 May/June 2014 |
| 23 | B | 1 | 0654/11 Oct/Nov 2015 |
| 24 | D | 1 | 0654/11 May/June 2016 |
| 25 | A | 1 | 0654/12 May/June 2016 |
| 26 | B | 1 | 0654/13 May/June 2016 |
| 27 | B | 1 | 0654/11 Oct/Nov 2016 |
| 28 | D | 1 | 0654/13 Oct/Nov 2016 |
| 29 | D | 1 | 0654/12 May/June 2017 |
| 30 | C | 1 | 0654/13 May/June 2017 |
| 31 | C | 1 | 0654/11 Oct/Nov 2017 |
| 32 | C | 1 | 0654/12 Oct/Nov 2017 |
| 33 | D | 1 | 0654/13 Oct/Nov 2017 |
| 34 | C | 1 | 0654/13 Oct/Nov 2017 |
| 35 | A | 1 | 0654/11 Oct/Nov 2018 |
| 36 | C | 1 | 0654/12 Oct/Nov 2018 |
| 37 | C | 1 | 0654/13 Oct/Nov 2018 |
| 38 | D | 1 | 0654/11 May/June 2019 |
| 39 | D | 1 | 0654/12 May/June 2019 |
| 40 | D | 1 | 0654/13 May/June 2019 |
| 41 | D | 1 | 0654/11 Oct/Nov 2019 |
| 42 | D | 1 | 0654/12 Oct/Nov 2019 |
| 43 | D | 1 | 0654/13 Oct/Nov 2019 |
| 44 | D | 1 | 0654/11 May/June 2020 |
| 45 | D | 1 | 0654/12 May/June 2020 |
| 46 | D | 1 | 0654/13 May/June 2020 |
| 47 | B | 1 | 0654/11 Oct/Nov 2020 |
| 48 | B | 1 | 0654/12 Oct/Nov 2020 |
| 49 | C | 1 | 0654/13 Oct/Nov 2020 |
| 50 | B | 1 | 0654/12 Feb/March 2021 |
| 51 | C | 1 | 0654/11 May/June 2021 |
| 52 | B | 1 | 0654/12 May/June 2021 |
| 53 | C | 1 | 0654/12 May/June 2021 |
| 54 | B | 1 | 0654/13 May/June 2021 |
| 55 | C | 1 | 0654/13 May/June 2021 |
| 56 | B | 1 | 0654/11 Oct/Nov 2021 |
| 57 | B | 1 | 0654/12 Oct/Nov 2021 |
| 58 | B | 1 | 0654/13 Oct/Nov 2021 |
| 59 | D | 1 | 0654/12 Feb/March 2022 |
| 60 | C | 1 | 0654/11 May/June 2022 |
| 61 | B | 1 | 0654/12 May/June 2022 |
| 62 | B | 1 | 0654/13 May/June 2022 |
| 63 | A | 1 | 0654/11 Oct/Nov 2022 |
| 64 | A | 1 | 0654/11 Oct/Nov 2022 |
| 65 | A | 1 | 0654/12 Oct/Nov 2022 |
| 66 | A | 1 | 0654/13 Oct/Nov 2022 |
| 67 | A | 1 | 0654/12 Feb/March 2023 |
| 68 | B | 1 | 0654/11 May/June 2023 |
| 69 | D | 1 | 0654/12 May/June 2023 |
| 70 | D | 1 | 0654/13 May/June 2023 |
| 71 | C | 1 | 0654/11 Oct/Nov 2023 |
| 72 | C | 1 | 0654/12 Oct/Nov 2023 |
| 73 | C | 1 | 0654/12 Oct/Nov 2023 |
| 74 | C | 1 | 0654/13 Oct/Nov 2023 |
| 75 | C | 1 | 0654/12 Feb/March 2024 |
| 76 | D | 1 | 0654/11 May/June 2024 |
| 77 | C | 1 | 0654/11 Oct/Nov 2024 |
| 78 | A | 1 | 0654/12 Oct/Nov 2024 |
| 79 | A | 1 | 0654/13 Oct/Nov 2024 |
| 80 | B | 1 | 0654/12 Feb/March 2025 |
| 81 | B | 1 | 0654/11 May/June 2025 |
| 82 | D | 1 | 0654/11 Oct/Nov 2025 |
| 83 | B | 1 | 0654/12 Oct/Nov 2025 |
| 84 | B | 1 | 0654/13 Oct/Nov 2025 |
7 A food contains reducing sugar, but no starch. What colours will be obtained if samples of the food are tested with Benedict's reagent and with iodine solution? Benedict's test iodine test A blue blue-black B blue brown C red-orange blue-black D red-orange brown
1 marks
Answer: D
7 Food tests are performed on four substances. Which substance contains fat and protein? test reagent Benedict's biuret ethanol iodine A v x x v B v v x x Cc x v v x D x x v v key ¥ = positive test result X = negative test result
1 marks
Answer: C
20 Glucose gives a red precipitate when tested with reagent X. Cellulose, a protein and starch are broken down into their monomers. Which of these monomers also give a red precipitate when tested with reagent X? cellulose protein starch 0 OO DW > ~*~ KOM Kk 4 \ 4 x
1 marks
Answer: C
17 A chemical from a plant is tested. damp litmus paper chemical from a plant and lime (CaO) heat An alkaline gas, ammonia (NH3), is produced. What is the chemical from the plant? A cellulose B a protein C starch D a sugar
1 marks
Answer: B
17 Proteins consist of long chains of …...1…... molecules and always contain the elements carbon, hydrogen, nitrogen and …...2…... . Which words correctly complete gaps 1 and 2? 1 2 A amino acid oxygen B amino acid sulfur C glucose oxygen D glucose sulfur
1 marks
Answer: A
15 Which molecules join into long chains to make proteins? A amino acids B ethene C glucose D starch
1 marks
Answer: A
5 Which cells produce starch in their cytoplasm? A all animal cells B all plant cells C some animal cells D some plant cells
1 marks
Answer: D
14 Which molecules join into long chains to make proteins? A amino acids B ethene C glucose D starch
1 marks
Answer: A
6 Tests were carried out on a clear liquid. The table shows the results. test result biuret purple colour ethanol white colour iodine brown colour What did the clear liquid contain? fat protein starch A Jv v v key B J v x v= yes Cc v x v X =no D Xx v v
1 marks
Answer: B
17 What is made when amino acids join together in a large chain? A cellulose B glucose C protein D starch
1 marks
Answer: C
7 Tests were carried out on a clear liquid. The table shows the results. test result biuret purple colour ethanol white colour iodine brown colour What did the clear liquid contain? fat protein starch A v v v B v v x Cc v x v D x v v key v/=yes X =no
1 marks
Answer: B
15 What is made when amino acids join together in a large chain? A cellulose B glucose C protein D starch
1 marks
Answer: C
7 Tests were carried out on a clear liquid. The table shows the results. test result biuret purple colour ethanol white colour iodine brown colour What did the clear liquid contain? fat protein starch A v v v B v v x Cc v x v D x v v key v/=yes X =no
1 marks
Answer: B
15 What is made when amino acids join together in a large chain? A cellulose B glucose C protein D starch
1 marks
Answer: C
17 In the diagram below, the compounds on the left are monomers and those on the right are polymers. amino acids 1 protein 2 3 glucose 4 starch Which two arrows link the monomer to the correct polymer? A 1 and 2 B 1 and 4 C 2 and 3 D 3 and 4
1 marks
Answer: B
16 In the diagram below, the compounds on the left are monomers and those on the right are polymers. amino acids 1 protein 2 3 glucose 4 starch Which two arrows link the monomer to the correct polymer? A 1 and 2 B 1 and 4 C 2 and 3 D 3 and 4
1 marks
Answer: B
6 Tests were carried out on a clear liquid, with the following results. test colour obtained Benedict’s blue biuret purple iodine blue/black What did the clear liquid contain? A protein only B protein and starch only C protein and reducing sugar only D protein, reducing sugar and starch
1 marks
Answer: B
5 The table shows the results of food tests on a breakfast cereal. test result Benedict’s bright orange iodine dark blue biuret pale blue ethanol slightly milky solution What do these results show? A The cereal helps to reduce body weight. B The cereal is a source of energy. C The cereal is a source of vitamin C. D The cereal promotes muscle growth.
1 marks
Answer: B
5 Tests were carried out on a clear liquid, with the following results. test colour obtained Benedict’s blue biuret purple iodine blue/black What did the clear liquid contain? A protein only B protein and starch only C protein and reducing sugar only D protein, reducing sugar and starch
1 marks
Answer: B
3 Which row shows a chemical molecule and the basic unit from which it is made? chemical molecule basic unit A glycogen amino acid B glycogen simple sugar C oil amino acid D oil simple sugar
1 marks
Answer: B
4 Which row shows a chemical molecule and the basic unit from which it is made? chemical molecule basic unit A glycogen amino acid B glycogen simple sugar C oil amino acid D oil simple sugar
1 marks
Answer: B
5 Which row states the basic units from which the large molecules are made? large molecules basic units A oil amino acids B oil simple sugars C protein amino acids D protein simple sugars
1 marks
Answer: C
6 Nutrient molecules are made up from smaller molecules. Nutrients can be identified by food tests. Which row correctly describes a protein? smaller molecules test which gives a positive result A amino acids Benedict’s test B amino acids biuret test C sugars Benedict’s test D sugars biuret test
1 marks
Answer: B
4 Which chemical element is found in proteins, but not in carbohydrates or fats? A carbon B hydrogen C oxygen D nitrogen
1 marks
Answer: D
4 What is needed in a cell to make a protein molecule? amino energy glycerol A v v x B v x v Cc x v x D x x v key v=yes X =no
1 marks
Answer: A
16 The structures of a carbohydrate and an amino acid are shown. OH OH H OH H H H C C C C C C H H2N C C OH O H H OH H OH H O carbohydrate amino acid Which elements are present in both structures? A carbon, hydrogen and nitrogen only B carbon, hydrogen and oxygen only C carbon, nitrogen and oxygen only D carbon, hydrogen, nitrogen and oxygen
1 marks
Answer: B
13 Which molecule contains carbon? A ammonia B fat C sulfuric acid D water
1 marks
Answer: B
3 Which result with the biuret test would show protein is present? A blue B green C orange D purple
1 marks
Answer: D
5 Enzymes are biological catalysts. To which group of compounds do enzymes belong? A carbohydrates B fats C hormones D proteins
1 marks
Answer: D
12 A biuret test on a sample of food is positive. Which nutrient does this result show to be present? A fat B glucose C protein D vitamin C
1 marks
Answer: C
8 Food tests are performed on four substances. Which substance contains fat and protein? test reagent Benedict's biuret ethanol iodine A Jv v x x B Jv x x Jv Cc x v v x D x x v v key ¥ = positive test result X = negative test result
1 marks
Answer: C
8 Food tests are performed on four substances. Which substance contains fat and protein? test reagent Benedict's biuret ethanol iodine A J J x x B J x x J Cc x J J x D x x Jv Jv key ¥ = positive test result X = negative test result
1 marks
Answer: C
1 What type of substances are enzymes? A carbohydrates B fats C lipids D proteins
1 marks
Answer: D
8 Food tests are performed on four substances. Which substance contains fat and protein? test reagent Benedict's biuret ethanol iodine A J J x x B J x x J Cc x J J x D x x Jv Jv key ¥ = positive test result X = negative test result
1 marks
Answer: C
4 Glycerol is a component of which large molecules? A fats B glycogen C proteins D starch
1 marks
Answer: A
3 Which element is found in proteins but not in carbohydrates and fats? A carbon B hydrogen C nitrogen D oxygen
1 marks
Answer: C
4 During which food test is heat required? A fats B protein C reducing sugars D starch
1 marks
Answer: C
3 Which chemical element is found in proteins, but not in carbohydrates or fats? A carbon B hydrogen C oxygen D nitrogen
1 marks
Answer: D
3 Which chemical element is found in proteins, but not in carbohydrates or fats? A carbon B hydrogen C oxygen D nitrogen
1 marks
Answer: D
3 Which chemical element is found in proteins, but not in carbohydrates or fats? A carbon B hydrogen C oxygen D nitrogen
1 marks
Answer: D
3 Which result with the biuret test shows that protein is present? A blue B green C orange D purple
1 marks
Answer: D
3 Which result with the biuret test shows that protein is present? A blue B green C orange D purple
1 marks
Answer: D
3 Which result with the biuret test shows that protein is present? A blue B green C orange D purple
1 marks
Answer: D
3 A food contains reducing sugar, but no starch. What colours will be obtained if samples of the food are tested with Benedict’s solution and with iodine solution? Benedict’s test iodine test A blue blue-black B blue brown C red-orange blue-black D red-orange brown
1 marks
Answer: D
3 A food contains reducing sugar, but no starch. What colours will be obtained if samples of the food are tested with Benedict’s solution and with iodine solution? Benedict’s test iodine test A blue blue-black B blue brown C red-orange blue-black D red-orange brown
1 marks
Answer: D
3 A food contains reducing sugar, but no starch. What colours will be obtained if samples of the food are tested with Benedict’s solution and with iodine solution? Benedict’s test iodine test A blue blue-black B blue brown C red-orange blue-black D red-orange brown
1 marks
Answer: D
3 Nutrient molecules are made up from smaller molecules. Nutrients can be identified by food tests. Which row is true for a protein? smaller molecules test which gives a positive result A amino acids Benedict’s test B amino acids biuret test C sugars Benedict’s test D sugars biuret test
1 marks
Answer: B
3 Which type of biological molecule contains carbon, hydrogen, oxygen and nitrogen? A fat B protein C reducing sugar D starch
1 marks
Answer: B
3 What colour does Benedict’s solution change to when heated with a reducing sugar? A blue B blue-black C orange D purple
1 marks
Answer: C
3 What are the molecules that make up fats and oils? A amino acids and glycerol B fatty acids and glycerol C glucose and amino acids D glucose and fatty acids
1 marks
Answer: B
3 Three food tests are carried out on a sample of food. The results are shown in the table. food test final colour Benedict’s blue biuret blue iodine blue-black From these results, which nutrient is in the food? A reducing sugar B protein C starch D vitamin C
1 marks
Answer: C
3 Which row matches the nutrient to the chemical elements that it contains? nutrient carbon hydrogen oxygen nitrogen A fat v v x x B protein Jv J Jv Jv Cc starch v x v v D sugar x v v v key / = contains element X = does not contain element
1 marks
Answer: B
4 Which type of molecule are enzymes? A fat B carbohydrate C protein D DNA
1 marks
Answer: C
3 Which row matches the nutrient to the chemical elements that it contains? nutrient carbon hydrogen oxygen nitrogen A fat v v x x B protein Jv J Jv Jv Cc starch v x v v D sugar x v v v key / = contains element X = does not contain element
1 marks
Answer: B
4 Which type of molecule are enzymes? A fat B carbohydrate C protein D DNA
1 marks
Answer: C
3 Which row shows a large molecule and a basic unit from which it is made? large molecule basic unit A glycogen amino acid B glycogen glucose C oil amino acid D oil glucose
1 marks
Answer: B
3 Which molecule contains carbon? A ammonia B fat C sulfuric acid D water
1 marks
Answer: B
3 Which molecule contains carbon? A ammonia B fat C sulfuric acid D water
1 marks
Answer: B
3 Which chemical element is found in all proteins, but not in all carbohydrates or fats? A carbon B hydrogen C oxygen D nitrogen
1 marks
Answer: D
3 Which small molecules are joined together to make a starch molecule? A amino acids B fatty acids C glucose D glycerol
1 marks
Answer: C
3 Which reagent is used to test for the presence of protein in a food sample? A Benedict’s solution B biuret C ethanol D iodine
1 marks
Answer: B
3 Which reagent is used to test for the presence of protein in a food sample? A Benedict’s solution B biuret C ethanol D iodine
1 marks
Answer: B
3 Glycerol is a component of which large molecules? A fats B glycogen C proteins D starch
1 marks
Answer: A
4 Which elements can be found in an enzyme molecule? 1 carbon 2 nitrogen 3 oxygen A 1, 2 and 3 B 1 and 3 only C 1 only D 2 only
1 marks
Answer: A
3 Glycerol is a component of which large molecules? A fats B glycogen C proteins D starch
1 marks
Answer: A
3 Glycerol is a component of which large molecules? A fats B glycogen C proteins D starch
1 marks
Answer: A
3 Tests are carried out on a colourless liquid. The results are shown. test resultant colour biuret purple ethanol emulsion white iodine brown Which food groups does the liquid contain? A protein and fat B protein and starch C reducing sugar and fat D starch and fat
1 marks
Answer: A
3 Linoleic acid is a fatty acid. Which larger molecule may contain linoleic acid? A glycogen B oil C protein D starch
1 marks
Answer: B
3 Which row shows the elements and the small molecules that are used to make the larger molecules? small larger elements molecule molecule A carbon, hydrogen and oxygen glucose fats B carbon, hydrogen, oxygen and nitrogen amino acids fats C carbon, hydrogen and oxygen glucose proteins D carbon, hydrogen, oxygen and nitrogen amino acids proteins
1 marks
Answer: D
3 Which row shows the elements and the small molecules that are used to make the larger molecules? small larger elements molecule molecule A carbon, hydrogen and oxygen glucose fats B carbon, hydrogen, oxygen and nitrogen amino acids fats C carbon, hydrogen and oxygen glucose proteins D carbon, hydrogen, oxygen and nitrogen amino acids proteins
1 marks
Answer: D
3 Which colour does Benedict’s solution change to when heated with a reducing sugar? A blue B blue-black C orange D purple
1 marks
Answer: C
3 A colourless liquid gives the test results shown. test colour obtained Benedict’s blue biuret purple iodine blue / black Which nutrients are in the colourless liquid? A protein, reducing sugar and starch B protein and reducing sugar only C protein and starch only D protein only
1 marks
Answer: C
4 Which type of molecule are enzymes? A carbohydrate B fat C protein D starch
1 marks
Answer: C
3 Which food test requires heating? A fat B protein C reducing sugar D starch
1 marks
Answer: C
3 Which smaller molecules is glycogen made from? A amino acids B fatty acids C glucose D glycerol
1 marks
Answer: C
3 Which smaller molecules make up larger fat molecules? A glucose and amino acids B glucose and fatty acids C glycerol and amino acids D glycerol and fatty acids
1 marks
Answer: D
3 Three food tests are carried out on a sample of food. The results are shown. food test final colour Benedict’s blue biuret blue iodine blue-black From these results, which nutrient is in the food? A reducing sugar B protein C starch D fat
1 marks
Answer: C
3 What is required to test for the presence of a reducing sugar? “solution | solution | Reet A J x J B x J J c J x x D x J x
1 marks
Answer: A
3 What is required to test for the presence of a reducing sugar? “solution | solution | Reet A J x J B x J J c J x x D x J x
1 marks
Answer: A
5 What is the test for the presence of protein in a food sample? A Benedict’s solution B biuret test C ethanol emulsion D iodine solution
1 marks
Answer: B
4 Which molecules make up fats and oils? A amino acids and glycerol B fatty acids and glycerol C glucose and amino acids D glucose and fatty acids
1 marks
Answer: B
4 Which row represents a food that contains a reducing sugar and a fat? purple with orange with milky-white blue-black with biuret reagent Benedict’s solution with ethanol iodine solution A yes no no yes B yes no yes no C no yes no yes D no yes yes no
1 marks
Answer: D
2 Which row matches the nutrient to the chemical elements that it contains? | nutrient fat protein starch glucose carbon hydrogen oxygen nitrogen
1 marks
Answer: B
2 Which row matches the nutrient to the chemical elements that it contains? | nutrient fat protein starch glucose carbon hydrogen oxygen nitrogen
1 marks
Answer: B