TopicalSciences - Co-ordinated (Double) 0654Biological moleculesBiological moleculesPaper 1

Biological molecules — Paper 1 · IGCSE Sciences - Co-ordinated (Double) 0654

B4.1· 84 questions · 84 marks · 101 min · 2006–2025· Multiple choice

Every Cambridge IGCSE Sciences - Co-ordinated (Double) Paper 1 question on biological molecules, laid out as 18 A4 pages with the mark scheme below. Nothing is left out. Free to read, no account.

Different topic or paper

Questions18 pages

Question 1: A food contains reducing sugar, but no starch. What colours will be obtained if samples of the food are tested with Benedict's reagent and …Question 2: Food tests are performed on four substances.

Which substance contains fat and protein?

test reagent

Benedict's biuret ethanol iodine
A v…Question 3: Glucose gives a red precipitate when tested with reagent X.
Cellulose, a protein and starch are broken down into their monomers.

Which of …1 / 18
Question 4: A chemical from a plant is tested. damp litmus paper chemical from a plant and lime (CaO) heat An alkaline gas, ammonia (NH3), is produced.…Question 5: Proteins consist of long chains of …...1…... molecules and always contain the elements carbon, hydrogen, nitrogen and …...2…... . Which wor…Question 6: Which molecules join into long chains to make proteins? A amino acids B ethene C glucose D starchQuestion 7: Which cells produce starch in their cytoplasm? A all animal cells B all plant cells C some animal cells D some plant cells2 / 18
Question 8: Which molecules join into long chains to make proteins? A amino acids B ethene C glucose D starchQuestion 9: Tests were carried out on a clear liquid. The table shows the results.

test

result

biuret purple colour
ethanol white colour
iodine brow…Question 10: What is made when amino acids join together in a large chain? A cellulose B glucose C protein D starchQuestion 11: Tests were carried out on a clear liquid. The table shows the results.

test result
biuret purple colour
ethanol white colour
iodine brown …3 / 18
Question 12: What is made when amino acids join together in a large chain? A cellulose B glucose C protein D starchQuestion 13: Tests were carried out on a clear liquid. The table shows the results.

test result
biuret purple colour
ethanol white colour
iodine brown …Question 14: What is made when amino acids join together in a large chain? A cellulose B glucose C protein D starchQuestion 15: In the diagram below, the compounds on the left are monomers and those on the right are polymers. amino acids 1 protein 2 3 glucose 4 starc…4 / 18
Question 16: In the diagram below, the compounds on the left are monomers and those on the right are polymers. amino acids 1 protein 2 3 glucose 4 starc…Question 17: Tests were carried out on a clear liquid, with the following results. test colour obtained Benedict’s blue biuret purple iodine blue/black …Question 18: The table shows the results of food tests on a breakfast cereal. test result Benedict’s bright orange iodine dark blue biuret pale blue eth…5 / 18
Question 19: Tests were carried out on a clear liquid, with the following results. test colour obtained Benedict’s blue biuret purple iodine blue/black …Question 20: Which row shows a chemical molecule and the basic unit from which it is made? chemical molecule basic unit A glycogen amino acid B glycogen…Question 21: Which row shows a chemical molecule and the basic unit from which it is made? chemical molecule basic unit A glycogen amino acid B glycogen…Question 22: Which row states the basic units from which the large molecules are made? large molecules basic units A oil amino acids B oil simple sugars…6 / 18
Question 23: Nutrient molecules are made up from smaller molecules. Nutrients can be identified by food tests. Which row correctly describes a protein? …Question 24: Which chemical element is found in proteins, but not in carbohydrates or fats? A carbon B hydrogen C oxygen D nitrogenQuestion 25: What is needed in a cell to make a protein molecule?

amino energy glycerol
A v v x
B v x v
Cc x v x
D x x v

key
v=yes
X =noQuestion 26: The structures of a carbohydrate and an amino acid are shown. OH OH H OH H H H C C C C C C H H2N C C OH O H H OH H OH H O carbohydrate amin…Question 27: Which molecule contains carbon? A ammonia B fat C sulfuric acid D water7 / 18
Question 28: Which result with the biuret test would show protein is present? A blue B green C orange D purpleQuestion 29: Enzymes are biological catalysts. To which group of compounds do enzymes belong? A carbohydrates B fats C hormones D proteinsQuestion 30: A biuret test on a sample of food is positive. Which nutrient does this result show to be present? A fat B glucose C protein D vitamin CQuestion 31: Food tests are performed on four substances.

Which substance contains fat and protein?

test reagent

Benedict's biuret ethanol iodine
A J…Question 32: Food tests are performed on four substances.

Which substance contains fat and protein?

test reagent

Benedict's biuret ethanol iodine
A J…8 / 18
Question 33: What type of substances are enzymes? A carbohydrates B fats C lipids D proteinsQuestion 34: Food tests are performed on four substances.

Which substance contains fat and protein?

test reagent

Benedict's biuret ethanol iodine
A J…Question 35: Glycerol is a component of which large molecules? A fats B glycogen C proteins D starchQuestion 36: Which element is found in proteins but not in carbohydrates and fats? A carbon B hydrogen C nitrogen D oxygenQuestion 37: During which food test is heat required? A fats B protein C reducing sugars D starchQuestion 38: Which chemical element is found in proteins, but not in carbohydrates or fats? A carbon B hydrogen C oxygen D nitrogen9 / 18
Question 39: Which chemical element is found in proteins, but not in carbohydrates or fats? A carbon B hydrogen C oxygen D nitrogenQuestion 40: Which chemical element is found in proteins, but not in carbohydrates or fats? A carbon B hydrogen C oxygen D nitrogenQuestion 41: Which result with the biuret test shows that protein is present? A blue B green C orange D purpleQuestion 42: Which result with the biuret test shows that protein is present? A blue B green C orange D purpleQuestion 43: Which result with the biuret test shows that protein is present? A blue B green C orange D purpleQuestion 44: A food contains reducing sugar, but no starch. What colours will be obtained if samples of the food are tested with Benedict’s solution and…10 / 18
Question 45: A food contains reducing sugar, but no starch. What colours will be obtained if samples of the food are tested with Benedict’s solution and…Question 46: A food contains reducing sugar, but no starch. What colours will be obtained if samples of the food are tested with Benedict’s solution and…Question 47: Nutrient molecules are made up from smaller molecules. Nutrients can be identified by food tests. Which row is true for a protein? smaller …Question 48: Which type of biological molecule contains carbon, hydrogen, oxygen and nitrogen? A fat B protein C reducing sugar D starchQuestion 49: What colour does Benedict’s solution change to when heated with a reducing sugar? A blue B blue-black C orange D purple11 / 18
Question 50: What are the molecules that make up fats and oils? A amino acids and glycerol B fatty acids and glycerol C glucose and amino acids D glucos…Question 51: Three food tests are carried out on a sample of food. The results are shown in the table. food test final colour Benedict’s blue biuret blu…Question 52: Which row matches the nutrient to the chemical elements that it contains?

nutrient carbon hydrogen oxygen nitrogen
A fat v v x x
B protein…Question 53: Which type of molecule are enzymes? A fat B carbohydrate C protein D DNA12 / 18
Question 54: Which row matches the nutrient to the chemical elements that it contains?

nutrient carbon hydrogen oxygen nitrogen
A fat v v x x
B protein…Question 55: Which type of molecule are enzymes? A fat B carbohydrate C protein D DNAQuestion 56: Which row shows a large molecule and a basic unit from which it is made? large molecule basic unit A glycogen amino acid B glycogen glucose…Question 57: Which molecule contains carbon? A ammonia B fat C sulfuric acid D waterQuestion 58: Which molecule contains carbon? A ammonia B fat C sulfuric acid D waterQuestion 59: Which chemical element is found in all proteins, but not in all carbohydrates or fats? A carbon B hydrogen C oxygen D nitrogen13 / 18
Question 60: Which small molecules are joined together to make a starch molecule? A amino acids B fatty acids C glucose D glycerolQuestion 61: Which reagent is used to test for the presence of protein in a food sample? A Benedict’s solution B biuret C ethanol D iodineQuestion 62: Which reagent is used to test for the presence of protein in a food sample? A Benedict’s solution B biuret C ethanol D iodineQuestion 63: Glycerol is a component of which large molecules? A fats B glycogen C proteins D starchQuestion 64: Which elements can be found in an enzyme molecule? 1 carbon 2 nitrogen 3 oxygen A 1, 2 and 3 B 1 and 3 only C 1 only D 2 onlyQuestion 65: Glycerol is a component of which large molecules? A fats B glycogen C proteins D starch14 / 18
Question 66: Glycerol is a component of which large molecules? A fats B glycogen C proteins D starchQuestion 67: Tests are carried out on a colourless liquid. The results are shown. test resultant colour biuret purple ethanol emulsion white iodine brow…Question 68: Linoleic acid is a fatty acid. Which larger molecule may contain linoleic acid? A glycogen B oil C protein D starchQuestion 69: Which row shows the elements and the small molecules that are used to make the larger molecules? small larger elements molecule molecule A …15 / 18
Question 70: Which row shows the elements and the small molecules that are used to make the larger molecules? small larger elements molecule molecule A …Question 71: Which colour does Benedict’s solution change to when heated with a reducing sugar? A blue B blue-black C orange D purpleQuestion 72: A colourless liquid gives the test results shown. test colour obtained Benedict’s blue biuret purple iodine blue / black Which nutrients ar…Question 73: Which type of molecule are enzymes? A carbohydrate B fat C protein D starchQuestion 74: Which food test requires heating? A fat B protein C reducing sugar D starch16 / 18
Question 75: Which smaller molecules is glycogen made from? A amino acids B fatty acids C glucose D glycerolQuestion 76: Which smaller molecules make up larger fat molecules? A glucose and amino acids B glucose and fatty acids C glycerol and amino acids D glyc…Question 77: Three food tests are carried out on a sample of food. The results are shown. food test final colour Benedict’s blue biuret blue iodine blue…Question 78: What is required to test for the presence of a reducing sugar?

“solution | solution | Reet
A J x J
B x J J
c J x x
D x J xQuestion 79: What is required to test for the presence of a reducing sugar?

“solution | solution | Reet
A J x J
B x J J
c J x x
D x J x17 / 18
Question 80: What is the test for the presence of protein in a food sample? A Benedict’s solution B biuret test C ethanol emulsion D iodine solutionQuestion 81: Which molecules make up fats and oils? A amino acids and glycerol B fatty acids and glycerol C glucose and amino acids D glucose and fatty …Question 82: Which row represents a food that contains a reducing sugar and a fat? purple with orange with milky-white blue-black with biuret reagent Be…Question 83: Which row matches the nutrient to the chemical elements that it contains?

| nutrient

fat
protein

starch

glucose

carbon

hydrogen

oxyg…Question 84: Which row matches the nutrient to the chemical elements that it contains?

| nutrient

fat
protein

starch

glucose

carbon

hydrogen

oxyg…18 / 18

Mark scheme84 answers

Answers below. Sit the paper first if you are practising.

Pastlit

Sciences - Co-ordinated (Double) 0654 · Biological molecules — Paper 1

IGCSE · topical answer key — answer key (teacher use)

Question

Answer

Marks

1D1
2C1
3C1
4B1
5A1
6A1
7D1
8A1
9B1
10C1
11B1
12C1
13B1
14C1
15B1
16B1
17B1
18B1
19B1
20B1
21B1
22C1
23B1
24D1
25A1
26B1
27B1
28D1
29D1
30C1
31C1
32C1
33D1
34C1
35A1
36C1
37C1
38D1
39D1
40D1
41D1
42D1
43D1
44D1
45D1
46D1
47B1
48B1
49C1
1 / 2

Pastlit

Sciences - Co-ordinated (Double) 0654 · Biological molecules — Paper 1

IGCSE · topical answer key — answer key (teacher use)

Question

Answer

Marks

50B1
51C1
52B1
53C1
54B1
55C1
56B1
57B1
58B1
59D1
60C1
61B1
62B1
63A1
64A1
65A1
66A1
67A1
68B1
69D1
70D1
71C1
72C1
73C1
74C1
75C1
76D1
77C1
78A1
79A1
80B1
81B1
82D1
83B1
84B1
2 / 2
QuestionAnswerMarksFrom
1D10654/11 May/June 2006
2C10654/11 Oct/Nov 2007
3C10654/11 Oct/Nov 2007
4B10654/11 Oct/Nov 2008
5A10654/11 May/June 2009
6A10654/12 Oct/Nov 2010
7D10654/13 Oct/Nov 2010
8A10654/13 Oct/Nov 2010
9B10654/11 May/June 2011
10C10654/11 May/June 2011
11B10654/12 May/June 2011
12C10654/12 May/June 2011
13B10654/13 May/June 2011
14C10654/13 May/June 2011
15B10654/11 Oct/Nov 2011
16B10654/13 Oct/Nov 2011
17B10654/11 May/June 2013
18B10654/12 May/June 2013
19B10654/13 May/June 2013
20B10654/11 Oct/Nov 2013
21B10654/12 Oct/Nov 2013
22C10654/12 May/June 2014
23B10654/11 Oct/Nov 2015
24D10654/11 May/June 2016
25A10654/12 May/June 2016
26B10654/13 May/June 2016
27B10654/11 Oct/Nov 2016
28D10654/13 Oct/Nov 2016
29D10654/12 May/June 2017
30C10654/13 May/June 2017
31C10654/11 Oct/Nov 2017
32C10654/12 Oct/Nov 2017
33D10654/13 Oct/Nov 2017
34C10654/13 Oct/Nov 2017
35A10654/11 Oct/Nov 2018
36C10654/12 Oct/Nov 2018
37C10654/13 Oct/Nov 2018
38D10654/11 May/June 2019
39D10654/12 May/June 2019
40D10654/13 May/June 2019
41D10654/11 Oct/Nov 2019
42D10654/12 Oct/Nov 2019
43D10654/13 Oct/Nov 2019
44D10654/11 May/June 2020
45D10654/12 May/June 2020
46D10654/13 May/June 2020
47B10654/11 Oct/Nov 2020
48B10654/12 Oct/Nov 2020
49C10654/13 Oct/Nov 2020
50B10654/12 Feb/March 2021
51C10654/11 May/June 2021
52B10654/12 May/June 2021
53C10654/12 May/June 2021
54B10654/13 May/June 2021
55C10654/13 May/June 2021
56B10654/11 Oct/Nov 2021
57B10654/12 Oct/Nov 2021
58B10654/13 Oct/Nov 2021
59D10654/12 Feb/March 2022
60C10654/11 May/June 2022
61B10654/12 May/June 2022
62B10654/13 May/June 2022
63A10654/11 Oct/Nov 2022
64A10654/11 Oct/Nov 2022
65A10654/12 Oct/Nov 2022
66A10654/13 Oct/Nov 2022
67A10654/12 Feb/March 2023
68B10654/11 May/June 2023
69D10654/12 May/June 2023
70D10654/13 May/June 2023
71C10654/11 Oct/Nov 2023
72C10654/12 Oct/Nov 2023
73C10654/12 Oct/Nov 2023
74C10654/13 Oct/Nov 2023
75C10654/12 Feb/March 2024
76D10654/11 May/June 2024
77C10654/11 Oct/Nov 2024
78A10654/12 Oct/Nov 2024
79A10654/13 Oct/Nov 2024
80B10654/12 Feb/March 2025
81B10654/11 May/June 2025
82D10654/11 Oct/Nov 2025
83B10654/12 Oct/Nov 2025
84B10654/13 Oct/Nov 2025

Another paper, or another topic

All of Biological molecules

Questions as text

Q1 · A food contains reducing sugar, but no starch 0654/11 May/June 2006

7 A food contains reducing sugar, but no starch. What colours will be obtained if samples of the food are tested with Benedict's reagent and with iodine solution? Benedict's test iodine test A blue blue-black B blue brown C red-orange blue-black D red-orange brown

1 marks

Answer: D

This question in 0654/11 May/June 2006

Q2 · Food tests are performed on four substances 0654/11 Oct/Nov 2007

7 Food tests are performed on four substances. Which substance contains fat and protein? test reagent Benedict's biuret ethanol iodine A v x x v B v v x x Cc x v v x D x x v v key ¥ = positive test result X = negative test result

1 marks

Answer: C

This question in 0654/11 Oct/Nov 2007

Q3 · Glucose gives a red precipitate when tested with reagent X 0654/11 Oct/Nov 2007

20 Glucose gives a red precipitate when tested with reagent X. Cellulose, a protein and starch are broken down into their monomers. Which of these monomers also give a red precipitate when tested with reagent X? cellulose protein starch 0 OO DW > ~*~ KOM Kk 4 \ 4 x

1 marks

Answer: C

This question in 0654/11 Oct/Nov 2007

Q4 · A chemical from a plant is tested 0654/11 Oct/Nov 2008

17 A chemical from a plant is tested. damp litmus paper chemical from a plant and lime (CaO) heat An alkaline gas, ammonia (NH3), is produced. What is the chemical from the plant? A cellulose B a protein C starch D a sugar

1 marks

Answer: B

This question in 0654/11 Oct/Nov 2008

Q5 · Proteins consist of long chains of …...1….. 0654/11 May/June 2009

17 Proteins consist of long chains of …...1…... molecules and always contain the elements carbon, hydrogen, nitrogen and …...2…... . Which words correctly complete gaps 1 and 2? 1 2 A amino acid oxygen B amino acid sulfur C glucose oxygen D glucose sulfur

1 marks

Answer: A

This question in 0654/11 May/June 2009

Q6 · Which molecules join into long chains to make proteins? 0654/12 Oct/Nov 2010

15 Which molecules join into long chains to make proteins? A amino acids B ethene C glucose D starch

1 marks

Answer: A

This question in 0654/12 Oct/Nov 2010

Q7 · Which cells produce starch in their cytoplasm? 0654/13 Oct/Nov 2010

5 Which cells produce starch in their cytoplasm? A all animal cells B all plant cells C some animal cells D some plant cells

1 marks

Answer: D

This question in 0654/13 Oct/Nov 2010

Q8 · Which molecules join into long chains to make proteins? 0654/13 Oct/Nov 2010

14 Which molecules join into long chains to make proteins? A amino acids B ethene C glucose D starch

1 marks

Answer: A

This question in 0654/13 Oct/Nov 2010

Q9 · Tests were carried out on a clear liquid 0654/11 May/June 2011

6 Tests were carried out on a clear liquid. The table shows the results. test result biuret purple colour ethanol white colour iodine brown colour What did the clear liquid contain? fat protein starch A Jv v v key B J v x v= yes Cc v x v X =no D Xx v v

1 marks

Answer: B

This question in 0654/11 May/June 2011

Q10 · What is made when amino acids join together in a large chain? 0654/11 May/June 2011

17 What is made when amino acids join together in a large chain? A cellulose B glucose C protein D starch

1 marks

Answer: C

This question in 0654/11 May/June 2011

Q11 · Tests were carried out on a clear liquid 0654/12 May/June 2011

7 Tests were carried out on a clear liquid. The table shows the results. test result biuret purple colour ethanol white colour iodine brown colour What did the clear liquid contain? fat protein starch A v v v B v v x Cc v x v D x v v key v/=yes X =no

1 marks

Answer: B

This question in 0654/12 May/June 2011

Q12 · What is made when amino acids join together in a large chain? 0654/12 May/June 2011

15 What is made when amino acids join together in a large chain? A cellulose B glucose C protein D starch

1 marks

Answer: C

This question in 0654/12 May/June 2011

Q13 · Tests were carried out on a clear liquid 0654/13 May/June 2011

7 Tests were carried out on a clear liquid. The table shows the results. test result biuret purple colour ethanol white colour iodine brown colour What did the clear liquid contain? fat protein starch A v v v B v v x Cc v x v D x v v key v/=yes X =no

1 marks

Answer: B

This question in 0654/13 May/June 2011

Q14 · What is made when amino acids join together in a large chain? 0654/13 May/June 2011

15 What is made when amino acids join together in a large chain? A cellulose B glucose C protein D starch

1 marks

Answer: C

This question in 0654/13 May/June 2011

Q15 · In the diagram below, the compounds on the left are monomers and those on the right are… 0654/11 Oct/Nov 2011

17 In the diagram below, the compounds on the left are monomers and those on the right are polymers. amino acids 1 protein 2 3 glucose 4 starch Which two arrows link the monomer to the correct polymer? A 1 and 2 B 1 and 4 C 2 and 3 D 3 and 4

1 marks

Answer: B

This question in 0654/11 Oct/Nov 2011

Q16 · In the diagram below, the compounds on the left are monomers and those on the right are… 0654/13 Oct/Nov 2011

16 In the diagram below, the compounds on the left are monomers and those on the right are polymers. amino acids 1 protein 2 3 glucose 4 starch Which two arrows link the monomer to the correct polymer? A 1 and 2 B 1 and 4 C 2 and 3 D 3 and 4

1 marks

Answer: B

This question in 0654/13 Oct/Nov 2011

Q17 · Tests were carried out on a clear liquid, with the following results 0654/11 May/June 2013

6 Tests were carried out on a clear liquid, with the following results. test colour obtained Benedict’s blue biuret purple iodine blue/black What did the clear liquid contain? A protein only B protein and starch only C protein and reducing sugar only D protein, reducing sugar and starch

1 marks

Answer: B

This question in 0654/11 May/June 2013

Q18 · The table shows the results of food tests on a breakfast cereal 0654/12 May/June 2013

5 The table shows the results of food tests on a breakfast cereal. test result Benedict’s bright orange iodine dark blue biuret pale blue ethanol slightly milky solution What do these results show? A The cereal helps to reduce body weight. B The cereal is a source of energy. C The cereal is a source of vitamin C. D The cereal promotes muscle growth.

1 marks

Answer: B

This question in 0654/12 May/June 2013

Q19 · Tests were carried out on a clear liquid, with the following results 0654/13 May/June 2013

5 Tests were carried out on a clear liquid, with the following results. test colour obtained Benedict’s blue biuret purple iodine blue/black What did the clear liquid contain? A protein only B protein and starch only C protein and reducing sugar only D protein, reducing sugar and starch

1 marks

Answer: B

This question in 0654/13 May/June 2013

Q20 · Which row shows a chemical molecule and the basic unit from which it is made? 0654/11 Oct/Nov 2013

3 Which row shows a chemical molecule and the basic unit from which it is made? chemical molecule basic unit A glycogen amino acid B glycogen simple sugar C oil amino acid D oil simple sugar

1 marks

Answer: B

This question in 0654/11 Oct/Nov 2013

Q21 · Which row shows a chemical molecule and the basic unit from which it is made? 0654/12 Oct/Nov 2013

4 Which row shows a chemical molecule and the basic unit from which it is made? chemical molecule basic unit A glycogen amino acid B glycogen simple sugar C oil amino acid D oil simple sugar

1 marks

Answer: B

This question in 0654/12 Oct/Nov 2013

Q22 · Which row states the basic units from which the large molecules are made? 0654/12 May/June 2014

5 Which row states the basic units from which the large molecules are made? large molecules basic units A oil amino acids B oil simple sugars C protein amino acids D protein simple sugars

1 marks

Answer: C

This question in 0654/12 May/June 2014

Q23 · Nutrient molecules are made up from smaller molecules 0654/11 Oct/Nov 2015

6 Nutrient molecules are made up from smaller molecules. Nutrients can be identified by food tests. Which row correctly describes a protein? smaller molecules test which gives a positive result A amino acids Benedict’s test B amino acids biuret test C sugars Benedict’s test D sugars biuret test

1 marks

Answer: B

This question in 0654/11 Oct/Nov 2015

Q24 · Which chemical element is found in proteins, but not in carbohydrates or fats? 0654/11 May/June 2016

4 Which chemical element is found in proteins, but not in carbohydrates or fats? A carbon B hydrogen C oxygen D nitrogen

1 marks

Answer: D

This question in 0654/11 May/June 2016

Q25 · What is needed in a cell to make a protein molecule? 0654/12 May/June 2016

4 What is needed in a cell to make a protein molecule? amino energy glycerol A v v x B v x v Cc x v x D x x v key v=yes X =no

1 marks

Answer: A

This question in 0654/12 May/June 2016

Q26 · The structures of a carbohydrate and an amino acid are shown 0654/13 May/June 2016

16 The structures of a carbohydrate and an amino acid are shown. OH OH H OH H H H C C C C C C H H2N C C OH O H H OH H OH H O carbohydrate amino acid Which elements are present in both structures? A carbon, hydrogen and nitrogen only B carbon, hydrogen and oxygen only C carbon, nitrogen and oxygen only D carbon, hydrogen, nitrogen and oxygen

1 marks

Answer: B

This question in 0654/13 May/June 2016

Q27 · Which molecule contains carbon? 0654/11 Oct/Nov 2016

13 Which molecule contains carbon? A ammonia B fat C sulfuric acid D water

1 marks

Answer: B

This question in 0654/11 Oct/Nov 2016

Q28 · Which result with the biuret test would show protein is present? 0654/13 Oct/Nov 2016

3 Which result with the biuret test would show protein is present? A blue B green C orange D purple

1 marks

Answer: D

This question in 0654/13 Oct/Nov 2016

Q29 · Enzymes are biological catalysts 0654/12 May/June 2017

5 Enzymes are biological catalysts. To which group of compounds do enzymes belong? A carbohydrates B fats C hormones D proteins

1 marks

Answer: D

This question in 0654/12 May/June 2017

Q30 · A biuret test on a sample of food is positive 0654/13 May/June 2017

12 A biuret test on a sample of food is positive. Which nutrient does this result show to be present? A fat B glucose C protein D vitamin C

1 marks

Answer: C

This question in 0654/13 May/June 2017

Q31 · Food tests are performed on four substances 0654/11 Oct/Nov 2017

8 Food tests are performed on four substances. Which substance contains fat and protein? test reagent Benedict's biuret ethanol iodine A Jv v x x B Jv x x Jv Cc x v v x D x x v v key ¥ = positive test result X = negative test result

1 marks

Answer: C

This question in 0654/11 Oct/Nov 2017

Q32 · Food tests are performed on four substances 0654/12 Oct/Nov 2017

8 Food tests are performed on four substances. Which substance contains fat and protein? test reagent Benedict's biuret ethanol iodine A J J x x B J x x J Cc x J J x D x x Jv Jv key ¥ = positive test result X = negative test result

1 marks

Answer: C

This question in 0654/12 Oct/Nov 2017

Q33 · What type of substances are enzymes? 0654/13 Oct/Nov 2017

1 What type of substances are enzymes? A carbohydrates B fats C lipids D proteins

1 marks

Answer: D

This question in 0654/13 Oct/Nov 2017

Q34 · Food tests are performed on four substances 0654/13 Oct/Nov 2017

8 Food tests are performed on four substances. Which substance contains fat and protein? test reagent Benedict's biuret ethanol iodine A J J x x B J x x J Cc x J J x D x x Jv Jv key ¥ = positive test result X = negative test result

1 marks

Answer: C

This question in 0654/13 Oct/Nov 2017

Q35 · Glycerol is a component of which large molecules? 0654/11 Oct/Nov 2018

4 Glycerol is a component of which large molecules? A fats B glycogen C proteins D starch

1 marks

Answer: A

This question in 0654/11 Oct/Nov 2018

Q36 · Which element is found in proteins but not in carbohydrates and fats? 0654/12 Oct/Nov 2018

3 Which element is found in proteins but not in carbohydrates and fats? A carbon B hydrogen C nitrogen D oxygen

1 marks

Answer: C

This question in 0654/12 Oct/Nov 2018

Q37 · During which food test is heat required? 0654/13 Oct/Nov 2018

4 During which food test is heat required? A fats B protein C reducing sugars D starch

1 marks

Answer: C

This question in 0654/13 Oct/Nov 2018

Q38 · Which chemical element is found in proteins, but not in carbohydrates or fats? 0654/11 May/June 2019

3 Which chemical element is found in proteins, but not in carbohydrates or fats? A carbon B hydrogen C oxygen D nitrogen

1 marks

Answer: D

This question in 0654/11 May/June 2019

Q39 · Which chemical element is found in proteins, but not in carbohydrates or fats? 0654/12 May/June 2019

3 Which chemical element is found in proteins, but not in carbohydrates or fats? A carbon B hydrogen C oxygen D nitrogen

1 marks

Answer: D

This question in 0654/12 May/June 2019

Q40 · Which chemical element is found in proteins, but not in carbohydrates or fats? 0654/13 May/June 2019

3 Which chemical element is found in proteins, but not in carbohydrates or fats? A carbon B hydrogen C oxygen D nitrogen

1 marks

Answer: D

This question in 0654/13 May/June 2019

Q41 · Which result with the biuret test shows that protein is present? 0654/11 Oct/Nov 2019

3 Which result with the biuret test shows that protein is present? A blue B green C orange D purple

1 marks

Answer: D

This question in 0654/11 Oct/Nov 2019

Q42 · Which result with the biuret test shows that protein is present? 0654/12 Oct/Nov 2019

3 Which result with the biuret test shows that protein is present? A blue B green C orange D purple

1 marks

Answer: D

This question in 0654/12 Oct/Nov 2019

Q43 · Which result with the biuret test shows that protein is present? 0654/13 Oct/Nov 2019

3 Which result with the biuret test shows that protein is present? A blue B green C orange D purple

1 marks

Answer: D

This question in 0654/13 Oct/Nov 2019

Q44 · A food contains reducing sugar, but no starch 0654/11 May/June 2020

3 A food contains reducing sugar, but no starch. What colours will be obtained if samples of the food are tested with Benedict’s solution and with iodine solution? Benedict’s test iodine test A blue blue-black B blue brown C red-orange blue-black D red-orange brown

1 marks

Answer: D

This question in 0654/11 May/June 2020

Q45 · A food contains reducing sugar, but no starch 0654/12 May/June 2020

3 A food contains reducing sugar, but no starch. What colours will be obtained if samples of the food are tested with Benedict’s solution and with iodine solution? Benedict’s test iodine test A blue blue-black B blue brown C red-orange blue-black D red-orange brown

1 marks

Answer: D

This question in 0654/12 May/June 2020

Q46 · A food contains reducing sugar, but no starch 0654/13 May/June 2020

3 A food contains reducing sugar, but no starch. What colours will be obtained if samples of the food are tested with Benedict’s solution and with iodine solution? Benedict’s test iodine test A blue blue-black B blue brown C red-orange blue-black D red-orange brown

1 marks

Answer: D

This question in 0654/13 May/June 2020

Q47 · Nutrient molecules are made up from smaller molecules 0654/11 Oct/Nov 2020

3 Nutrient molecules are made up from smaller molecules. Nutrients can be identified by food tests. Which row is true for a protein? smaller molecules test which gives a positive result A amino acids Benedict’s test B amino acids biuret test C sugars Benedict’s test D sugars biuret test

1 marks

Answer: B

This question in 0654/11 Oct/Nov 2020

Q48 · Which type of biological molecule contains carbon, hydrogen, oxygen and nitrogen? 0654/12 Oct/Nov 2020

3 Which type of biological molecule contains carbon, hydrogen, oxygen and nitrogen? A fat B protein C reducing sugar D starch

1 marks

Answer: B

This question in 0654/12 Oct/Nov 2020

Q49 · What colour does Benedict’s solution change to when heated with a reducing sugar? 0654/13 Oct/Nov 2020

3 What colour does Benedict’s solution change to when heated with a reducing sugar? A blue B blue-black C orange D purple

1 marks

Answer: C

This question in 0654/13 Oct/Nov 2020

Q50 · What are the molecules that make up fats and oils? 0654/12 Feb/March 2021

3 What are the molecules that make up fats and oils? A amino acids and glycerol B fatty acids and glycerol C glucose and amino acids D glucose and fatty acids

1 marks

Answer: B

This question in 0654/12 Feb/March 2021

Q51 · Three food tests are carried out on a sample of food 0654/11 May/June 2021

3 Three food tests are carried out on a sample of food. The results are shown in the table. food test final colour Benedict’s blue biuret blue iodine blue-black From these results, which nutrient is in the food? A reducing sugar B protein C starch D vitamin C

1 marks

Answer: C

This question in 0654/11 May/June 2021

Q52 · Which row matches the nutrient to the chemical elements that it contains? 0654/12 May/June 2021

3 Which row matches the nutrient to the chemical elements that it contains? nutrient carbon hydrogen oxygen nitrogen A fat v v x x B protein Jv J Jv Jv Cc starch v x v v D sugar x v v v key / = contains element X = does not contain element

1 marks

Answer: B

This question in 0654/12 May/June 2021

Q53 · Which type of molecule are enzymes? 0654/12 May/June 2021

4 Which type of molecule are enzymes? A fat B carbohydrate C protein D DNA

1 marks

Answer: C

This question in 0654/12 May/June 2021

Q54 · Which row matches the nutrient to the chemical elements that it contains? 0654/13 May/June 2021

3 Which row matches the nutrient to the chemical elements that it contains? nutrient carbon hydrogen oxygen nitrogen A fat v v x x B protein Jv J Jv Jv Cc starch v x v v D sugar x v v v key / = contains element X = does not contain element

1 marks

Answer: B

This question in 0654/13 May/June 2021

Q55 · Which type of molecule are enzymes? 0654/13 May/June 2021

4 Which type of molecule are enzymes? A fat B carbohydrate C protein D DNA

1 marks

Answer: C

This question in 0654/13 May/June 2021

Q56 · Which row shows a large molecule and a basic unit from which it is made? 0654/11 Oct/Nov 2021

3 Which row shows a large molecule and a basic unit from which it is made? large molecule basic unit A glycogen amino acid B glycogen glucose C oil amino acid D oil glucose

1 marks

Answer: B

This question in 0654/11 Oct/Nov 2021

Q57 · Which molecule contains carbon? 0654/12 Oct/Nov 2021

3 Which molecule contains carbon? A ammonia B fat C sulfuric acid D water

1 marks

Answer: B

This question in 0654/12 Oct/Nov 2021

Q58 · Which molecule contains carbon? 0654/13 Oct/Nov 2021

3 Which molecule contains carbon? A ammonia B fat C sulfuric acid D water

1 marks

Answer: B

This question in 0654/13 Oct/Nov 2021

Q59 · Which chemical element is found in all proteins, but not in all carbohydrates or fats? 0654/12 Feb/March 2022

3 Which chemical element is found in all proteins, but not in all carbohydrates or fats? A carbon B hydrogen C oxygen D nitrogen

1 marks

Answer: D

This question in 0654/12 Feb/March 2022

Q60 · Which small molecules are joined together to make a starch molecule? 0654/11 May/June 2022

3 Which small molecules are joined together to make a starch molecule? A amino acids B fatty acids C glucose D glycerol

1 marks

Answer: C

This question in 0654/11 May/June 2022

Q61 · Which reagent is used to test for the presence of protein in a food sample? 0654/12 May/June 2022

3 Which reagent is used to test for the presence of protein in a food sample? A Benedict’s solution B biuret C ethanol D iodine

1 marks

Answer: B

This question in 0654/12 May/June 2022

Q62 · Which reagent is used to test for the presence of protein in a food sample? 0654/13 May/June 2022

3 Which reagent is used to test for the presence of protein in a food sample? A Benedict’s solution B biuret C ethanol D iodine

1 marks

Answer: B

This question in 0654/13 May/June 2022

Q63 · Glycerol is a component of which large molecules? 0654/11 Oct/Nov 2022

3 Glycerol is a component of which large molecules? A fats B glycogen C proteins D starch

1 marks

Answer: A

This question in 0654/11 Oct/Nov 2022

Q64 · Which elements can be found in an enzyme molecule? 0654/11 Oct/Nov 2022

4 Which elements can be found in an enzyme molecule? 1 carbon 2 nitrogen 3 oxygen A 1, 2 and 3 B 1 and 3 only C 1 only D 2 only

1 marks

Answer: A

This question in 0654/11 Oct/Nov 2022

Q65 · Glycerol is a component of which large molecules? 0654/12 Oct/Nov 2022

3 Glycerol is a component of which large molecules? A fats B glycogen C proteins D starch

1 marks

Answer: A

This question in 0654/12 Oct/Nov 2022

Q66 · Glycerol is a component of which large molecules? 0654/13 Oct/Nov 2022

3 Glycerol is a component of which large molecules? A fats B glycogen C proteins D starch

1 marks

Answer: A

This question in 0654/13 Oct/Nov 2022

Q67 · Tests are carried out on a colourless liquid 0654/12 Feb/March 2023

3 Tests are carried out on a colourless liquid. The results are shown. test resultant colour biuret purple ethanol emulsion white iodine brown Which food groups does the liquid contain? A protein and fat B protein and starch C reducing sugar and fat D starch and fat

1 marks

Answer: A

This question in 0654/12 Feb/March 2023

Q68 · Linoleic acid is a fatty acid 0654/11 May/June 2023

3 Linoleic acid is a fatty acid. Which larger molecule may contain linoleic acid? A glycogen B oil C protein D starch

1 marks

Answer: B

This question in 0654/11 May/June 2023

Q69 · Which row shows the elements and the small molecules that are used to make the larger… 0654/12 May/June 2023

3 Which row shows the elements and the small molecules that are used to make the larger molecules? small larger elements molecule molecule A carbon, hydrogen and oxygen glucose fats B carbon, hydrogen, oxygen and nitrogen amino acids fats C carbon, hydrogen and oxygen glucose proteins D carbon, hydrogen, oxygen and nitrogen amino acids proteins

1 marks

Answer: D

This question in 0654/12 May/June 2023

Q70 · Which row shows the elements and the small molecules that are used to make the larger… 0654/13 May/June 2023

3 Which row shows the elements and the small molecules that are used to make the larger molecules? small larger elements molecule molecule A carbon, hydrogen and oxygen glucose fats B carbon, hydrogen, oxygen and nitrogen amino acids fats C carbon, hydrogen and oxygen glucose proteins D carbon, hydrogen, oxygen and nitrogen amino acids proteins

1 marks

Answer: D

This question in 0654/13 May/June 2023

Q71 · Which colour does Benedict’s solution change to when heated with a reducing sugar? 0654/11 Oct/Nov 2023

3 Which colour does Benedict’s solution change to when heated with a reducing sugar? A blue B blue-black C orange D purple

1 marks

Answer: C

This question in 0654/11 Oct/Nov 2023

Q72 · A colourless liquid gives the test results shown 0654/12 Oct/Nov 2023

3 A colourless liquid gives the test results shown. test colour obtained Benedict’s blue biuret purple iodine blue / black Which nutrients are in the colourless liquid? A protein, reducing sugar and starch B protein and reducing sugar only C protein and starch only D protein only

1 marks

Answer: C

This question in 0654/12 Oct/Nov 2023

Q73 · Which type of molecule are enzymes? 0654/12 Oct/Nov 2023

4 Which type of molecule are enzymes? A carbohydrate B fat C protein D starch

1 marks

Answer: C

This question in 0654/12 Oct/Nov 2023

Q74 · Which food test requires heating? 0654/13 Oct/Nov 2023

3 Which food test requires heating? A fat B protein C reducing sugar D starch

1 marks

Answer: C

This question in 0654/13 Oct/Nov 2023

Q75 · Which smaller molecules is glycogen made from? 0654/12 Feb/March 2024

3 Which smaller molecules is glycogen made from? A amino acids B fatty acids C glucose D glycerol

1 marks

Answer: C

This question in 0654/12 Feb/March 2024

Q76 · Which smaller molecules make up larger fat molecules? 0654/11 May/June 2024

3 Which smaller molecules make up larger fat molecules? A glucose and amino acids B glucose and fatty acids C glycerol and amino acids D glycerol and fatty acids

1 marks

Answer: D

This question in 0654/11 May/June 2024

Q77 · Three food tests are carried out on a sample of food 0654/11 Oct/Nov 2024

3 Three food tests are carried out on a sample of food. The results are shown. food test final colour Benedict’s blue biuret blue iodine blue-black From these results, which nutrient is in the food? A reducing sugar B protein C starch D fat

1 marks

Answer: C

This question in 0654/11 Oct/Nov 2024

Q78 · What is required to test for the presence of a reducing sugar? 0654/12 Oct/Nov 2024

3 What is required to test for the presence of a reducing sugar? “solution | solution | Reet A J x J B x J J c J x x D x J x

1 marks

Answer: A

This question in 0654/12 Oct/Nov 2024

Q79 · What is required to test for the presence of a reducing sugar? 0654/13 Oct/Nov 2024

3 What is required to test for the presence of a reducing sugar? “solution | solution | Reet A J x J B x J J c J x x D x J x

1 marks

Answer: A

This question in 0654/13 Oct/Nov 2024

Q80 · What is the test for the presence of protein in a food sample? 0654/12 Feb/March 2025

5 What is the test for the presence of protein in a food sample? A Benedict’s solution B biuret test C ethanol emulsion D iodine solution

1 marks

Answer: B

This question in 0654/12 Feb/March 2025

Q81 · Which molecules make up fats and oils? 0654/11 May/June 2025

4 Which molecules make up fats and oils? A amino acids and glycerol B fatty acids and glycerol C glucose and amino acids D glucose and fatty acids

1 marks

Answer: B

This question in 0654/11 May/June 2025

Q82 · Which row represents a food that contains a reducing sugar and a fat? 0654/11 Oct/Nov 2025

4 Which row represents a food that contains a reducing sugar and a fat? purple with orange with milky-white blue-black with biuret reagent Benedict’s solution with ethanol iodine solution A yes no no yes B yes no yes no C no yes no yes D no yes yes no

1 marks

Answer: D

This question in 0654/11 Oct/Nov 2025

Q83 · Which row matches the nutrient to the chemical elements that it contains? 0654/12 Oct/Nov 2025

2 Which row matches the nutrient to the chemical elements that it contains? | nutrient fat protein starch glucose carbon hydrogen oxygen nitrogen

1 marks

Answer: B

This question in 0654/12 Oct/Nov 2025

Q84 · Which row matches the nutrient to the chemical elements that it contains? 0654/13 Oct/Nov 2025

2 Which row matches the nutrient to the chemical elements that it contains? | nutrient fat protein starch glucose carbon hydrogen oxygen nitrogen

1 marks

Answer: B

This question in 0654/13 Oct/Nov 2025