C12.2· 23 questions · 23 marks · 28 min · 2017–2025· Multiple choice
Every Cambridge IGCSE Science - Combined Paper 2 question on chromatography, laid out as 10 A4 pages with the mark scheme below. Nothing is left out. Free to read, no account.

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10 / 10Answers below. Sit the paper first if you are practising.
Pastlit
Science - Combined 0653 · Chromatography — Paper 2
IGCSE · topical answer key — answer key (teacher use)
Question
Answer
Marks
| Question | Answer | Marks | From |
|---|---|---|---|
| 1 | D | 1 | 0653/21 May/June 2017 |
| 2 | D | 1 | 0653/22 May/June 2017 |
| 3 | D | 1 | 0653/23 May/June 2017 |
| 4 | D | 1 | 0653/23 Oct/Nov 2017 |
| 5 | B | 1 | 0653/21 Oct/Nov 2018 |
| 6 | A | 1 | 0653/22 Oct/Nov 2018 |
| 7 | D | 1 | 0653/23 Oct/Nov 2018 |
| 8 | C | 1 | 0653/21 Oct/Nov 2019 |
| 9 | C | 1 | 0653/22 Oct/Nov 2019 |
| 10 | C | 1 | 0653/23 Oct/Nov 2019 |
| 11 | D | 1 | 0653/22 Feb/March 2020 |
| 12 | A | 1 | 0653/22 May/June 2020 |
| 13 | C | 1 | 0653/21 Oct/Nov 2020 |
| 14 | C | 1 | 0653/23 Oct/Nov 2020 |
| 15 | C | 1 | 0653/23 May/June 2021 |
| 16 | B | 1 | 0653/22 May/June 2024 |
| 17 | D | 1 | 0653/21 Oct/Nov 2024 |
| 18 | A | 1 | 0653/22 Feb/March 2025 |
| 19 | C | 1 | 0653/21 May/June 2025 |
| 20 | D | 1 | 0653/22 May/June 2025 |
| 21 | C | 1 | 0653/23 May/June 2025 |
| 22 | A | 1 | 0653/22 Oct/Nov 2025 |
| 23 | C | 1 | 0653/23 Oct/Nov 2025 |
14 Which diagram shows how a mixture of dyes in a food colouring are separated? A B filter paper filter paper spot of food colouring food colouring water solution C D spot of food colouring filter paper filter paper spot of food colouring water water
1 marks
Answer: D
14 Which diagram shows how a mixture of dyes in a food colouring are separated? A B filter paper filter paper spot of food colouring food colouring water solution C D spot of food colouring filter paper filter paper spot of food colouring water water
1 marks
Answer: D
14 Which diagram shows how a mixture of dyes in a food colouring are separated? A B filter paper filter paper spot of food colouring food colouring water solution C D spot of food colouring filter paper filter paper spot of food colouring water water
1 marks
Answer: D
15 Chromatography separates ink into different colours. Which diagram shows how the apparatus is set up? A B beaker beaker paper ink ink paper water water C D beaker beaker paper paper ink ink water water
1 marks
Answer: D
15 Four dyes are separated using chromatography. The results are shown. 1 2 3 4 Which dyes contain two colours that are present in both dyes? A 1 and 2 B 1 and 4 C 2 and 3 D 2 and 4
1 marks
Answer: B
15 The apparatus used for chromatography is shown. beaker chromatography paper sample pencil line spots solvent Which statement about the method used for chromatography is not correct? A The beaker is swirled to help the solvent to rise. B The chromatography paper is placed in the beaker after the solvent has been added. C The chromatography paper is removed before the solvent reaches the top of the paper. D The sample spots are placed on the pencil line above the level of the solvent.
1 marks
Answer: A
15 Which diagram shows how apparatus is used to separate the different colours in an ink? A B chromatography paper chromatography paper pencil line ink spot ink spot pencil line water water C D chromatography chromatography paper paper ink line ink spot pencil line ink spot water water
1 marks
Answer: D
14 A chromatogram of substance Q is shown. solvent front 20 cm 18 cm 16 cm 14 cm 12 cm 10 cm 8 cm 6 cm 4 cm 2 cm base line 0 cm Q What is the Rf value of Q? A 0.2 B 0.4 C 0.8 D 1.6
1 marks
Answer: C
14 The chromatogram of a black ink containing three dyes is shown. solvent front blue purple 20 cm 16 cm 12 cm yellow baseline 4 cm What is the Rf value of the purple ink? A 0.2 B 0.4 C 0.6 D 1.67
1 marks
Answer: C
14 Chromatography is carried out on three solutions P, Q and R. The chromatogram obtained is shown. solvent front base line P Q R Which statement is not correct? A P contains at least two substances. B Q contains the substance with the highest Rf value. C R is insoluble in the solvent. D P, Q and R together may contain only three substances.
1 marks
Answer: C
15 A dye contains four different coloured components. The dye is separated by chromatography. The table shows the colour and Rf values of the four coloured components. colour Rf value blue 0.50 green 0.52 red 0.74 yellow 0.36 Which two dyes are furthest apart from each other on the final chromatogram? A blue and green B blue and yellow C green and red D red and yellow
1 marks
Answer: D
14 Which process uses Rf values to identify the components of a mixture? A chromatography B crystallisation C distillation D filtration
1 marks
Answer: A
15 Two different dyes are analysed using chromatography. Each dye produces only one coloured spot on the chromatogram. The Rf values of the coloured spots are shown. coloured Rf value spot red 0.2 blue 0.4 The two different dyes are then mixed together to make a purple dye. What is observed on the chromatogram of the purple dye? A one spot with Rf value 0.3 B one spot with Rf value 0.6 C two spots with Rf values 0.2 and 0.4 D three spots with Rf values 0.2, 0.3 and 0.4
1 marks
Answer: C
15 A pure sample of a coloured dye is tested using chromatography. The chromatogram obtained is shown. solvent front P Q R baseline S How is the Rf value of the dye calculated? R R R S R S A P B Q C D Q S P S
1 marks
Answer: C
15 During a chromatography investigation, colour X moves 4.5 cm up the chromatography paper from the base line. The Rf value of colour X is 0.59. What is the distance moved by the solvent in this experiment? A 2.7 cm B 4.5 cm C 7.6 cm D 10.3 cm
1 marks
Answer: C
14 The diagram shows a chromatogram of an ink sample after separation by paper chromatography. solvent front coloured spots start line Which calculation is used to determine the Rf value of a coloured spot on the chromatogram? distance travelled by the solvent from the start line A distance travelled by the spot from the start line distance travelled by the spot from the start line B distance travelled by the solvent from the start line distance from the bottom of the chromatography paper to the spot C distance from the bottom to the top of the chromatography paper distance from the bottom to the top of the chromatography paper D distance from the bottom of the chromatography paper to the spot
1 marks
Answer: B
14 The diagram shows a chromatogram produced by four different substances. Which pure substance has an Rf value of 0.8? solvent front 5 4 3 cm 2 1 baseline 0 A B C D
1 marks
Answer: D
24 Dyes J and K are analysed using chromatography. Dye J contains coloured substances, P, Q and R. Dye K contains coloured substances, S and T. solvent front R T Q P S baseline J K Which conclusions can be made using the chromatogram? 1 Dye K does not contain substance Q. 2 Dye J contains at least three substances. 3 The Rf of Q is less than the Rf of S. 4 R and T are different substances. A 1 and 2 B 1 and 3 C 2 and 4 D 3 and 4
1 marks
Answer: A
23 A chromatogram of dyes X, Y and Z is shown. solvent front 8 7 6 5 distance from 4 baseline / cm 3 2 1 baseline 0 X Y Z Which statement about the chromatogram is correct? A Dye Y has the spot with the lowest Rf value. B Every dye is a mixture of colours. C The dyes X, Y and Z do not contain the same colour. D The spot in dye X has an Rf value of 4.
1 marks
Answer: C
25 The chromatogram of four different substances is shown. Which substance has an Rf value of 0.6? solvent front cm 20 18 16 14 12 10 8 6 4 baseline 2 0 A B C D
1 marks
Answer: D
25 The diagram shows a chromatogram for a brown ink. cm solvent front 14 blue dye 12 10 yellow dye 8 6 4 red dye 2 baseline 0 brown ink What is the Rf value of the blue dye? A 0.15 B 0.54 C 0.85 D 1.18
1 marks
Answer: C
25 How is an Rf value calculated in chromatography? distance travelled by substance A distance travelled by solvent distance travelled by solvent B distance travelled by substance distance from the baseline to the top of the paper C distance travelled by substance distance travelled by substance D distance from the baseline to the top of the paper
1 marks
Answer: A
26 A chromatography experiment is carried out on a compound. The Rf value of the compound is 0.60. Which diagram shows the chromatogram of the compound? A B C D 10 12 12 10 8 6 8 6 2 2 2 2
1 marks
Answer: C