TopicalBiology (9-1) 0970Biological moleculesBiological moleculesPaper 1

Biological molecules — Paper 1 · IGCSE Biology (9-1) 0970

4.1· 43 questions · 43 marks · 52 min · 2018–2025· Multiple choice

Every Cambridge IGCSE Biology (9-1) Paper 1 question on biological molecules, laid out as 10 A4 pages with the mark scheme below. Nothing is left out. Free to read, no account.

Different topic or paper

Questions10 pages

Question 1: The table shows the results of food tests carried out on a fruit. test Benedict’s biuret ethanol iodine result positive positive negative n…Question 2: The data show the concentrations of sugar and starch in an onion. total sugar including starch reducing sugar / g per 100g / g per 100g 3.7…Question 3: Four solutions were tested to see whether they contained protein, starch or glucose. The colours of the solutions after the tests are recor…1 / 10
Question 4: Small molecules are used as the basic units in the synthesis of large food molecules. Which statement is correct? A Amino acids are basic u…Question 5: Which identifies the chemical elements found in proteins?

carbon hydrogen oxygen nitrogen

v v v v key

J/ = present

0 0O WwW >

J WA J x…Question 6: A student carries out a test to see whether the solution in a test-tube contains protein. Which row shows the correct food test and a posit…Question 7: The pie charts show the composition of 100 g of four different foods. Which food provides the most energy? A B C D key carbohydrate fats an…Question 8: Which food-testing solution shows a positive result when it turns from blue to purple? A Benedict’s solution B biuret solution C ethanol D …2 / 10
Question 9: What is the definition of an enzyme? A a carbohydrate that acts as a catalyst B a DNA molecule that acts as a catalyst C a fat that acts as…Question 10: Which food-testing solution shows a positive result when it turns from blue to purple? A Benedict’s solution B biuret solution C ethanol D …Question 11: Which substances are made by linking together glucose molecules only? A cellulose, glycogen and starch B fats, cellulose and proteins C pro…Question 12: What kind of molecule is an enzyme? A fat B glucose C protein D starchQuestion 13: The graph shows how the percentage of protein found in potatoes was affected by the concentration of nitrate fertiliser added to the crop w…3 / 10
Question 14: Which element is found in proteins but not carbohydrates? A carbon B hydrogen C nitrogen D oxygenQuestion 15: What are the products when proteins are broken down? A amino acids B fatty acids C glycerol D simple sugarsQuestion 16: What is the dietary importance of carbohydrates? A to promote healthy bones and teeth B to make fats C to provide energy for the body D to …Question 17: What are the smaller basic units of starch and glycogen molecules? starch glycogen A amino acids fatty acids and glycerol B amino acids glu…Question 18: Some terms used to describe enzyme-controlled reactions are listed. 1 catalyst 2 product 3 protein 4 substrate Which terms describe an enzy…4 / 10
Question 19: The diagram shows two food tests being carried out on solution X. test 1 test 2 biuret Benedict’s solution solution red colour heat solutio…Question 20: What are the smaller basic units of starch and glycogen molecules? starch glycogen A amino acids fatty acids and glycerol B amino acids glu…Question 21: Which type of molecule are enzymes made of? A carbohydrates B fats C proteins D vitamins5 / 10
Question 22: The diagram shows two food tests being carried out on solution X. test 1 test 2 biuret Benedict’s solution solution red colour heat solutio…Question 23: Which chemical is used to test for the presence of protein in a food sample? A Benedict’s solution B biuret solution C DCPIP D iodine solut…Question 24: The diagram shows part of a protein molecule. X What does X represent? A amino acid B fatty acid C glycerol D sugarQuestion 25: What is a role of water in digestion? A It is an enzyme. B It is a solvent. C It is soluble. D It is a mineral.6 / 10
Question 26: The diagram represents a protein molecule. What do the small circles represent? A amino acids B fatty acids C glycerol D simple sugarsQuestion 27: Which row identifies the chemical elements in proteins?

carbon hydrogen oxygen nitrogen
A v J J J key
B Jv x Jv x / = present
Cc x v v v X…Question 28: The table shows some large biological molecules and some small biological molecules. large biological small biological molecules molecules …Question 29: Large biological molecules are made of smaller molecules. Which row correctly matches the large molecule with the smaller molecules? large …7 / 10
Question 30: A student tests a solution for protein. Which row shows the correct food test and a positive result? colour seen with a name of test positi…Question 31: Iodine solution is added to a food sample that contains starch. What is the colour of the sample after the iodine solution has been added? …Question 32: Which of these statements about enzymes are correct? 1 enzymes are proteins 2 enzymes are used up during chemical reactions 3 enzymes speed…Question 33: A sample of food was tested with biuret reagent. Which colour would be seen if the food sample contained protein? A blue-black B brick red …Question 34: Which elements are found in fats? A carbon, hydrogen and iron B carbon, hydrogen and oxygen C carbon, hydrogen and nitrogen D carbon, oxyge…8 / 10
Question 35: Which statements about enzymes are correct? 1 Enzymes are biological catalysts. 2 Enzymes are carbohydrates. 3 Enzymes have an active site.…Question 36: Which carbohydrate is used to make cell walls? A cellulose B glycogen C starch D sucroseQuestion 37: What are the products of protein digestion in the stomach? A amino acids B fatty acids C glycerol D proteasesQuestion 38: What do genes code for? A fats B proteins C starch D sugarsQuestion 39: Which row correctly shows the elements and small molecules that are found in a large molecule? elements small molecules large molecule A ca…Question 40: Which substance is the DCPIP test used for? A fat B protein C starch D vitamin C9 / 10
Question 41: Small molecules are used to synthesise large food molecules. Which statement is correct? A Amino acids are the basic units of carbohydrates…Question 42: A student used biuret reagent to test milk for the presence of protein. The milk tested positive for protein. Which colour did the sample c…Question 43: Enzymes are large molecules. Which small molecules are they made from? A amino acids B carbon dioxide and water C fatty acids D sugars10 / 10

Mark scheme43 answers

Answers below. Sit the paper first if you are practising.

Pastlit

Biology (9-1) 0970 · Biological molecules — Paper 1

IGCSE · topical answer key — answer key (teacher use)

Question

Answer

Marks

1C1
2D1
3C1
4C1
5A1
6D1
7B1
8B1
9D1
10B1
11A1
12C1
13A1
14C1
15A1
16C1
17D1
18A1
19B1
20D1
21C1
22B1
23B1
24A1
25B1
26A1
27A1
28B1
29C1
30D1
31A1
32C1
33D1
34B1
35B1
36A1
37A1
38B1
39C1
40D1
41C1
42D1
43A1
1 / 1
QuestionAnswerMarksFrom
1C10970/11 May/June 2018
2D10970/12 Oct/Nov 2018
3C10970/11 May/June 2019
4C10970/11 May/June 2019
5A10970/12 Oct/Nov 2019
6D10970/12 Oct/Nov 2019
7B10970/12 Oct/Nov 2019
8B10970/11 May/June 2020
9D10970/11 May/June 2020
10B10970/12 May/June 2020
11A10970/12 Oct/Nov 2020
12C10970/12 Oct/Nov 2020
13A10970/12 Oct/Nov 2020
14C10970/11 May/June 2021
15A10970/11 May/June 2021
16C10970/12 Oct/Nov 2021
17D10970/11 May/June 2022
18A10970/11 May/June 2022
19B10970/11 May/June 2022
20D10970/12 May/June 2022
21C10970/12 May/June 2022
22B10970/12 May/June 2022
23B10970/12 Oct/Nov 2022
24A10970/11 May/June 2023
25B10970/12 May/June 2023
26A10970/12 May/June 2023
27A10970/12 Oct/Nov 2023
28B10970/12 Oct/Nov 2023
29C10970/11 May/June 2024
30D10970/11 May/June 2024
31A10970/12 May/June 2024
32C10970/12 May/June 2024
33D10970/12 Oct/Nov 2024
34B10970/12 Oct/Nov 2024
35B10970/12 Oct/Nov 2024
36A10970/12 Oct/Nov 2024
37A10970/12 Oct/Nov 2024
38B10970/12 Oct/Nov 2024
39C10970/11 May/June 2025
40D10970/11 May/June 2025
41C10970/12 May/June 2025
42D10970/12 May/June 2025
43A10970/12 Oct/Nov 2025

Another paper, or another topic

Paper
Paper 143 questionsPaper 232 questionsPaper 3questions comingPaper 4questions coming

All of Biological molecules

Questions as text

Q1 · The table shows the results of food tests carried out on a fruit 0970/11 May/June 2018

9 The table shows the results of food tests carried out on a fruit. test Benedict’s biuret ethanol iodine result positive positive negative negative What did the fruit contain? A fat and reducing sugar B fat and starch C protein and reducing sugar D protein and starch

1 marks

Answer: C

This question in 0970/11 May/June 2018

Q2 · The data show the concentrations of sugar and starch in an onion 0970/12 Oct/Nov 2018

9 The data show the concentrations of sugar and starch in an onion. total sugar including starch reducing sugar / g per 100g / g per 100g 3.7 0.0 The onion is tested with Benedict’s solution and iodine solution. Which set of results is correct? Benedict’s iodine solution solution A blue blue-black B blue brown C brick red blue-black D brick red brown

1 marks

Answer: D

This question in 0970/12 Oct/Nov 2018

Q3 · Four solutions were tested to see whether they contained protein, starch or glucose 0970/11 May/June 2019

8 Four solutions were tested to see whether they contained protein, starch or glucose. The colours of the solutions after the tests are recorded in the table. Which solution contained protein and glucose but not starch? biuret test iodine test Benedict’s test A blue brown orange B blue blue-black blue C purple brown orange D purple blue-black blue

1 marks

Answer: C

This question in 0970/11 May/June 2019

Q4 · Small molecules are used as the basic units in the synthesis of large food molecules 0970/11 May/June 2019

9 Small molecules are used as the basic units in the synthesis of large food molecules. Which statement is correct? A Amino acids are basic units of carbohydrates. B Fatty acids are basic units of glycogen. C Glycerol is a basic unit of oils. D Simple sugar is a basic unit of protein.

1 marks

Answer: C

This question in 0970/11 May/June 2019

Q5 · Which identifies the chemical elements found in proteins? 0970/12 Oct/Nov 2019

8 Which identifies the chemical elements found in proteins? carbon hydrogen oxygen nitrogen v v v v key J/ = present 0 0O WwW > J WA J x Jv x Jv x X = absent x Jv x WA

1 marks

Answer: A

This question in 0970/12 Oct/Nov 2019

Q6 · A student carries out a test to see whether the solution in a test-tube contains protein 0970/12 Oct/Nov 2019

9 A student carries out a test to see whether the solution in a test-tube contains protein. Which row shows the correct food test and a positive result? colour seen with a name of test positive result A Benedict’s test blue-black B Benedict’s test purple C biuret test blue-black D biuret test purple

1 marks

Answer: D

This question in 0970/12 Oct/Nov 2019

Q7 · The pie charts show the composition of 100 g of four different foods 0970/12 Oct/Nov 2019

12 The pie charts show the composition of 100 g of four different foods. Which food provides the most energy? A B C D key carbohydrate fats and oils protein water

1 marks

Answer: B

This question in 0970/12 Oct/Nov 2019

Q8 · Which food-testing solution shows a positive result when it turns from blue to purple? 0970/11 May/June 2020

8 Which food-testing solution shows a positive result when it turns from blue to purple? A Benedict’s solution B biuret solution C ethanol D iodine solution

1 marks

Answer: B

This question in 0970/11 May/June 2020

Q9 · What is the definition of an enzyme? 0970/11 May/June 2020

9 What is the definition of an enzyme? A a carbohydrate that acts as a catalyst B a DNA molecule that acts as a catalyst C a fat that acts as a catalyst D a protein that acts as a catalyst

1 marks

Answer: D

This question in 0970/11 May/June 2020

Q10 · Which food-testing solution shows a positive result when it turns from blue to purple? 0970/12 May/June 2020

8 Which food-testing solution shows a positive result when it turns from blue to purple? A Benedict’s solution B biuret solution C ethanol D iodine solution

1 marks

Answer: B

This question in 0970/12 May/June 2020

Q11 · Which substances are made by linking together glucose molecules only? 0970/12 Oct/Nov 2020

9 Which substances are made by linking together glucose molecules only? A cellulose, glycogen and starch B fats, cellulose and proteins C proteins, oils and glycogen D starch, fats and oils

1 marks

Answer: A

This question in 0970/12 Oct/Nov 2020

Q12 · What kind of molecule is an enzyme? 0970/12 Oct/Nov 2020

11 What kind of molecule is an enzyme? A fat B glucose C protein D starch

1 marks

Answer: C

This question in 0970/12 Oct/Nov 2020

Q13 · The graph shows how the percentage of protein found in potatoes was affected by the… 0970/12 Oct/Nov 2020

13 The graph shows how the percentage of protein found in potatoes was affected by the concentration of nitrate fertiliser added to the crop when it was growing. percentage of protein in potatoes concentration of nitrate fertiliser added These results suggest that greater concentrations of nitrate fertiliser allow the potatoes to make more A amino acids. B cellulose. C lipid. D starch.

1 marks

Answer: A

This question in 0970/12 Oct/Nov 2020

Q14 · Which element is found in proteins but not carbohydrates? 0970/11 May/June 2021

9 Which element is found in proteins but not carbohydrates? A carbon B hydrogen C nitrogen D oxygen

1 marks

Answer: C

This question in 0970/11 May/June 2021

Q15 · What are the products when proteins are broken down? 0970/11 May/June 2021

15 What are the products when proteins are broken down? A amino acids B fatty acids C glycerol D simple sugars

1 marks

Answer: A

This question in 0970/11 May/June 2021

Q16 · What is the dietary importance of carbohydrates? 0970/12 Oct/Nov 2021

14 What is the dietary importance of carbohydrates? A to promote healthy bones and teeth B to make fats C to provide energy for the body D to make proteins

1 marks

Answer: C

This question in 0970/12 Oct/Nov 2021

Q17 · What are the smaller basic units of starch and glycogen molecules? 0970/11 May/June 2022

9 What are the smaller basic units of starch and glycogen molecules? starch glycogen A amino acids fatty acids and glycerol B amino acids glucose C glucose fatty acids and glycerol D glucose glucose

1 marks

Answer: D

This question in 0970/11 May/June 2022

Q18 · Some terms used to describe enzyme-controlled reactions are listed 0970/11 May/June 2022

10 Some terms used to describe enzyme-controlled reactions are listed. 1 catalyst 2 product 3 protein 4 substrate Which terms describe an enzyme? A 1 and 3 B 1 and 4 C 2 and 3 D 2 and 4

1 marks

Answer: A

This question in 0970/11 May/June 2022

Q19 · The diagram shows two food tests being carried out on solution X 0970/11 May/June 2022

11 The diagram shows two food tests being carried out on solution X. test 1 test 2 biuret Benedict’s solution solution red colour heat solution X purple solution X colour Which nutrients are present in solution X? A protein and starch B protein and sugar C starch and fat D starch and sugar

1 marks

Answer: B

This question in 0970/11 May/June 2022

Q20 · What are the smaller basic units of starch and glycogen molecules? 0970/12 May/June 2022

9 What are the smaller basic units of starch and glycogen molecules? starch glycogen A amino acids fatty acids and glycerol B amino acids glucose C glucose fatty acids and glycerol D glucose glucose

1 marks

Answer: D

This question in 0970/12 May/June 2022

Q21 · Which type of molecule are enzymes made of? 0970/12 May/June 2022

10 Which type of molecule are enzymes made of? A carbohydrates B fats C proteins D vitamins

1 marks

Answer: C

This question in 0970/12 May/June 2022

Q22 · The diagram shows two food tests being carried out on solution X 0970/12 May/June 2022

11 The diagram shows two food tests being carried out on solution X. test 1 test 2 biuret Benedict’s solution solution red colour heat solution X purple solution X colour Which nutrients are present in solution X? A protein and starch B protein and sugar C starch and fat D starch and sugar

1 marks

Answer: B

This question in 0970/12 May/June 2022

Q23 · Which chemical is used to test for the presence of protein in a food sample? 0970/12 Oct/Nov 2022

9 Which chemical is used to test for the presence of protein in a food sample? A Benedict’s solution B biuret solution C DCPIP D iodine solution

1 marks

Answer: B

This question in 0970/12 Oct/Nov 2022

Q24 · The diagram shows part of a protein molecule 0970/11 May/June 2023

9 The diagram shows part of a protein molecule. X What does X represent? A amino acid B fatty acid C glycerol D sugar

1 marks

Answer: A

This question in 0970/11 May/June 2023

Q25 · What is a role of water in digestion? 0970/12 May/June 2023

7 What is a role of water in digestion? A It is an enzyme. B It is a solvent. C It is soluble. D It is a mineral.

1 marks

Answer: B

This question in 0970/12 May/June 2023

Q26 · The diagram represents a protein molecule 0970/12 May/June 2023

9 The diagram represents a protein molecule. What do the small circles represent? A amino acids B fatty acids C glycerol D simple sugars

1 marks

Answer: A

This question in 0970/12 May/June 2023

Q27 · Which row identifies the chemical elements in proteins? 0970/12 Oct/Nov 2023

8 Which row identifies the chemical elements in proteins? carbon hydrogen oxygen nitrogen A v J J J key B Jv x Jv x / = present Cc x v v v X = absent D J J x x

1 marks

Answer: A

This question in 0970/12 Oct/Nov 2023

Q28 · The table shows some large biological molecules and some small biological molecules 0970/12 Oct/Nov 2023

9 The table shows some large biological molecules and some small biological molecules. large biological small biological molecules molecules 1 cellulose glucose 2 oil amino acids 3 glycogen glucose 4 protein ethanol Which rows correctly pair large molecules with the smaller molecules used to make them? A 1 and 2 B 1 and 3 C 2 and 3 D 3 and 4

1 marks

Answer: B

This question in 0970/12 Oct/Nov 2023

Q29 · Large biological molecules are made of smaller molecules 0970/11 May/June 2024

9 Large biological molecules are made of smaller molecules. Which row correctly matches the large molecule with the smaller molecules? large molecule smaller molecules A cellulose fatty acids B fat amino acids C protein amino acids D starch fatty acids

1 marks

Answer: C

This question in 0970/11 May/June 2024

Q30 · A student tests a solution for protein 0970/11 May/June 2024

10 A student tests a solution for protein. Which row shows the correct food test and a positive result? colour seen with a name of test positive result A Benedict’s test blue-black B Benedict’s test purple C biuret test blue-black D biuret test purple

1 marks

Answer: D

This question in 0970/11 May/June 2024

Q31 · Iodine solution is added to a food sample that contains starch 0970/12 May/June 2024

10 Iodine solution is added to a food sample that contains starch. What is the colour of the sample after the iodine solution has been added? A blue-black B purple C red D yellow

1 marks

Answer: A

This question in 0970/12 May/June 2024

Q32 · Which of these statements about enzymes are correct? 0970/12 May/June 2024

12 Which of these statements about enzymes are correct? 1 enzymes are proteins 2 enzymes are used up during chemical reactions 3 enzymes speed up metabolic reactions A 1, 2 and 3 B 1 and 2 only C 1 and 3 only D 2 and 3 only

1 marks

Answer: C

This question in 0970/12 May/June 2024

Q33 · A sample of food was tested with biuret reagent 0970/12 Oct/Nov 2024

10 A sample of food was tested with biuret reagent. Which colour would be seen if the food sample contained protein? A blue-black B brick red C yellow-brown D purple

1 marks

Answer: D

This question in 0970/12 Oct/Nov 2024

Q34 · Which elements are found in fats? 0970/12 Oct/Nov 2024

11 Which elements are found in fats? A carbon, hydrogen and iron B carbon, hydrogen and oxygen C carbon, hydrogen and nitrogen D carbon, oxygen and nitrogen

1 marks

Answer: B

This question in 0970/12 Oct/Nov 2024

Q35 · Which statements about enzymes are correct? 0970/12 Oct/Nov 2024

12 Which statements about enzymes are correct? 1 Enzymes are biological catalysts. 2 Enzymes are carbohydrates. 3 Enzymes have an active site. A 1 and 2 B 1 and 3 C 2 and 3 D 3 only

1 marks

Answer: B

This question in 0970/12 Oct/Nov 2024

Q36 · Which carbohydrate is used to make cell walls? 0970/12 Oct/Nov 2024

14 Which carbohydrate is used to make cell walls? A cellulose B glycogen C starch D sucrose

1 marks

Answer: A

This question in 0970/12 Oct/Nov 2024

Q37 · What are the products of protein digestion in the stomach? 0970/12 Oct/Nov 2024

19 What are the products of protein digestion in the stomach? A amino acids B fatty acids C glycerol D proteases

1 marks

Answer: A

This question in 0970/12 Oct/Nov 2024

Q38 · What do genes code for? 0970/12 Oct/Nov 2024

36 What do genes code for? A fats B proteins C starch D sugars

1 marks

Answer: B

This question in 0970/12 Oct/Nov 2024

Q39 · Which row correctly shows the elements and small molecules that are found in a large… 0970/11 May/June 2025

9 Which row correctly shows the elements and small molecules that are found in a large molecule? elements small molecules large molecule A carbon, hydrogen, oxygen, nitrogen glucose fat B carbon, hydrogen, oxygen, nitrogen glycerol fat C carbon, hydrogen, oxygen glucose starch D carbon, hydrogen, oxygen glycerol starch

1 marks

Answer: C

This question in 0970/11 May/June 2025

Q40 · Which substance is the DCPIP test used for? 0970/11 May/June 2025

10 Which substance is the DCPIP test used for? A fat B protein C starch D vitamin C

1 marks

Answer: D

This question in 0970/11 May/June 2025

Q41 · Small molecules are used to synthesise large food molecules 0970/12 May/June 2025

7 Small molecules are used to synthesise large food molecules. Which statement is correct? A Amino acids are the basic units of carbohydrates. B Fatty acids are the basic units of glycogen. C Glycerol is a basic unit of oils. D Simple sugar is the basic unit of protein.

1 marks

Answer: C

This question in 0970/12 May/June 2025

Q42 · A student used biuret reagent to test milk for the presence of protein 0970/12 May/June 2025

8 A student used biuret reagent to test milk for the presence of protein. The milk tested positive for protein. Which colour did the sample change to? A blue B blue-black C brick red D purple

1 marks

Answer: D

This question in 0970/12 May/June 2025

Q43 · Enzymes are large molecules 0970/12 Oct/Nov 2025

7 Enzymes are large molecules. Which small molecules are they made from? A amino acids B carbon dioxide and water C fatty acids D sugars

1 marks

Answer: A

This question in 0970/12 Oct/Nov 2025