21.1· 11 questions · 11 marks · 13 min · 2019–2025· Multiple choice
Every Cambridge IGCSE Biology (9-1) Paper 2 question on biotechnology and genetic modification, laid out as 3 A4 pages with the mark scheme below. Nothing is left out. Free to read, no account.





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3 / 3Answers below. Sit the paper first if you are practising.
Pastlit
Biology (9-1) 0970 · Biotechnology and genetic modification — Paper 2
IGCSE · topical answer key — answer key (teacher use)
Question
Answer
Marks
| Question | Answer | Marks | From |
|---|---|---|---|
| 1 | D | 1 | 0970/22 May/June 2019 |
| 2 | B | 1 | 0970/22 May/June 2020 |
| 3 | C | 1 | 0970/22 May/June 2020 |
| 4 | B | 1 | 0970/21 May/June 2021 |
| 5 | C | 1 | 0970/21 May/June 2021 |
| 6 | C | 1 | 0970/22 Oct/Nov 2021 |
| 7 | A | 1 | 0970/21 May/June 2022 |
| 8 | A | 1 | 0970/22 May/June 2022 |
| 9 | A | 1 | 0970/22 May/June 2023 |
| 10 | A | 1 | 0970/21 May/June 2025 |
| 11 | D | 1 | 0970/22 May/June 2025 |
38 All organisms share the same genetic code. This means that bacteria can be used to A improve the health of the digestive system. B manufacture biofuels in large quantities. C produce foods such as yoghurt and cheese. D make proteins using human DNA.
1 marks
Answer: D
37 What is the role of anaerobic respiration in bread-making? A to produce alcohol to flavour the bread B to produce gas to make the bread rise C to release enough energy to bake the bread D to release enough lactic acid to kill the yeast
1 marks
Answer: B
39 Which is a reason for using bacteria in biotechnology? A Bacteria are found inside the human body. B Bacteria do not become resistant to antibiotics. C Bacteria can make complex molecules. D Bacteria reproduce slowly.
1 marks
Answer: C
36 New strains of the crop plant wheat can be produced by cross-breeding disease resistant plants with those that give high yields of grain. What is this an example of? A natural selection B artificial selection C genetic engineering D asexual reproduction
1 marks
Answer: B
39 Why are bacteria useful in genetic engineering? A Their genetic code is different to other organisms. B They have cell walls. C They have plasmids. D They reproduce sexually.
1 marks
Answer: C
37 What is a characteristic of bacteria that makes them useful in genetic engineering? A The genetic code of bacteria is different to plants and animals. B Their nucleus contains DNA. C Plasmids can be transferred between cells. D They have large numbers of mitochondria.
1 marks
Answer: C
38 Which process makes use of a genetically engineered organism? A using bacteria to produce insulin B using enzymes in biological washing powders C using pectinase in fruit juice production D using yeast to produce ethanol
1 marks
Answer: A
38 Which process makes use of a genetically engineered organism? A using bacteria to produce insulin B using enzymes in biological washing powders C using pectinase in fruit juice production D using yeast to produce ethanol
1 marks
Answer: A
39 The diagram shows a bacterial cell containing a plasmid. plasmid What is inserted into the plasmid if this cell is to be used for the production of insulin? A a length of DNA from a human B a length of DNA from another bacterium C a molecule of insulin D an enzyme
1 marks
Answer: A
40 Some of the processes involved in the production of insulin by genetic modification are listed. 1 cutting of bacterial plasmid DNA with restriction enzymes 2 expression in bacteria of the human gene to make insulin 3 insertion of recombinant plasmids into bacteria 4 multiplication of bacteria containing recombinant plasmids In which order do these processes occur? A 1 3 4 2 B 2 1 3 4 C 3 1 2 4 D 4 1 3 2
1 marks
Answer: A
40 Which features make bacteria useful in biotechnology? 1 They are unaffected by antibiotics. 2 They do not contain plasmids. 3 They make complex molecules. 4 They reproduce rapidly. A 1 and 2 B 1 and 4 C 2 and 3 D 3 and 4
1 marks
Answer: D