Cambridge A Level Biology 9700 — 2016 Oct/Nov Paper 1 · Variant 2

This document consists of 16 printed pages. IB16 11_9700_12/3RP © UCLES 2016 [Turn over *1003441361* Cambridge International Examinations Cambridge International Advanced Subsidiary and Advanced Level BIOLOGY 9700/12 Paper 1 Multiple Choice October/November 2016 1 hour Additional Materials: Multiple Choice Answer Sheet Soft clean eraser Soft pencil (type B or HB is recommended) READ THESE INSTRUCTIONS FIRST Write in soft pencil. Do not use staples, paper clips, glue or correction fluid. Write your name, Centre number and candidate number on the Answer Sheet in the spaces provided unless this has been done for you. DO NOT WRITE IN ANY BARCODES. There are forty questions on this paper. Answer all questions. For each question there are four possible answers A, B, C and D. Choose the one you consider correct and record your choice in soft pencil on the separate Answer Sheet. Read the instructions on the Answer Sheet very carefully. Each correct answer will score one mark. A mark will not be deducted for a wrong answer. Any rough working should be done in this booklet. Electronic calculators may be used.

2 © UCLES 2016 9700/12/O/N/16 1 Until recently, the typical viruses known to science were 20 – 150 nm in size. In 2003, the Mimivirus was discovered with a size of approximately 680 nm. In 2013, the Pandoravirus was discovered which has a size of over 1000 nm. Which viruses can be seen using both a light microscope with a maximum resolution of 0.25 µm and an electron microscope? typical virus Mimivirus Pandoravirus A    key B    = can be seen C    = cannot be seen D    2 This electron micrograph of a section of a leaf has a magnification of × 210. X Y What is the actual length along the line X–Y? A 2.43 µm B 24.3 µm C 243.0 µm D 2430 µm 3 What is the correct order in which organelles function to make and secrete an enzyme? A nucleolus → ribosome → Golgi body → vesicle B nucleolus → smooth endoplasmic reticulum → lysosome → vesicle C nucleus → rough endoplasmic reticulum → Golgi body → vesicle D nucleus → smooth endoplasmic reticulum → lysosome → vesicle

3 © UCLES 2016 9700/12/O/N/16 [Turn over 4 The diagram shows a stage micrometer scale, with divisions 0.1 mm apart, viewed through an eyepiece containing a graticule. 0 10 20 30 40 50 60 70 80 90 10 What is the area of the field of view of the microscope at this magnification? (π = 3.14) A π × 12.5 × 12.5 = 4.9 × 102 µm2 B π × 50 × 50 = 7.9 × 103 µm2 C π × 125 × 125 = 4.9 × 104 µm2 D π × 250 × 250 = 2.0 × 105 µm2

4 © UCLES 2016 9700/12/O/N/16 5 A scientist carried out an experiment to separate the organelles in an animal cell by mass. The scientist mixed the cells with a buffer solution which had the same water potential as the cells. He then broke the cells open with a blender to release the organelles. The extracted mixture was filtered and then spun in a centrifuge at a high speed to separate the heaviest organelle. This sank to the bottom, forming a solid pellet, 1. solid pellet liquid above pellet The liquid above pellet 1 was poured into a clean centrifuge tube and spun in the centrifuge at a higher speed to separate the next heaviest organelle. This organelle sank to the bottom, forming a solid pellet, 2. He repeated this procedure twice more to obtain pellet 3 and pellet 4, each containing a single organelle. What is the function of the organelle extracted in pellet 2? A digestion of old organelles B production of ATP C production of mRNA D synthesis of protein 6 Which is a correct comparison between a typical prokaryotic cell and a typical eukaryotic cell? prokaryote eukaryote A circular DNA only linear DNA only B naked DNA DNA associated with protein C rough endoplasmic reticulum present smooth endoplasmic reticulum and rough endoplasmic reticulum present D ribosomes approximately 22 nm diameter ribosomes approximately 18 nm in diameter

5 © UCLES 2016 9700/12/O/N/16 [Turn over 7 The diagrams show four monosaccharides with the formula C6H12O6. OH 1 H HO H HO H OH H OH H OH H CH2OH H OH O 2 H H OH H CH2OH H OH O 3 H HO HO H CH2OH H OH H OH O 4 H HO H OH H OH H CH2OH H O Which diagrams do not show glucose molecules? A 1 and 2 B 1 and 4 C 2 and 3 D 3 and 4 8 Complete digestion of polysaccharides requires all the glycosidic bonds between the monomers to be broken. Amylase breaks only α-1,4 glycosidic bonds. How completely can amylase digest molecules of amylose, amylopectin or glycogen? carbohydrate amylose amylopectin glycogen A + +++ ++ key B + ++ +++ + some digestion C ++ + +++ ++ more digestion D +++ ++ + +++ most digestion 9 Which statements about a triglyceride molecule are correct? 1 It always contains unsaturated hydrocarbon tails. 2 It is formed using ester bonds. 3 It does not form hydrogen bonds. 4 It contains a hydrophilic glycerol head. A 1, 2 and 3 B 2, 3 and 4 C 1 and 4 only D 2 and 3 only

6 © UCLES 2016 9700/12/O/N/16 10 The table shows some features of collagen and haemoglobin. Which row is collagen? type of protein number of polypeptides solubility in water A fibrous three insoluble B fibrous four soluble C globular three insoluble D globular four soluble 11 The ring structure of sugars can also be represented as a linear structure, as shown in the diagram. C H CH2OH C OH H C OH HO C H H C OH H O Which of the sugar molecules could be represented by this formula? 1 β-glucose 2 ribose 3 sucrose A 1 and 2 only B 2 and 3 only C 1 only D 2 only 12 What is the minimum number of carbon atoms in an amino acid? A 1 B 2 C 3 D 4

7 © UCLES 2016 9700/12/O/N/16 [Turn over 13 HIV-1 protease is an enzyme produced by the HIV virus. Two identical chains of 99 amino acids form the enzyme. In each chain, amino acids 25, 26 and 27 in the sequence form part of the active site. Which orders of protein structure control the shape of the active site? A primary, secondary, tertiary and quaternary B primary, secondary and tertiary only C primary and quaternary only D quaternary only 14 The graph shows the results of an investigation into the effect of amylase on starch at three different temperatures. 0 concentration of reducing sugar / arbitrary units 30 time / minutes 60 50 °C 60 °C 70 °C Which statements are correct conclusions using these results? 1 The optimum temperature is 50 °C. 2 The initial rate of reaction is highest at 70 °C. 3 The higher the temperature the more quickly the enzyme denatures. A 1, 2 and 3 B 1 and 2 only C 1 and 3 only D 2 and 3 only 15 Substances called furanocoumarins are found in some fruits, where they act as inhibitors of the enzyme CYP3A4. This enzyme is needed to break down toxins in the human digestive system, so dangerous levels of these toxins may develop if these fruits are eaten. What does this information suggest about molecules of the enzyme CYP3A4? A They lower the activation energy of the toxin breakdown process. B They show specificity for a particular substrate found in fruits. C They will change permanently when acted upon by furanocoumarin molecules. D They will resume normal activity when levels of furanocoumarins decrease.

8 © UCLES 2016 9700/12/O/N/16 16 Which observations support a link between active transport and ATP production? 1 Active transport occurs only in living cells. 2 The rate of active transport decreases in low oxygen concentrations. 3 Companion cells have large numbers of mitochondria. A 1, 2 and 3 B 1 and 2 only C 2 and 3 only D 3 only 17 A method called freeze-fracture can be used to study the structure of cell membranes. The membrane is frozen and then split down the middle, separating the two layers of phospholipids from each other. Any proteins contained within one layer remain in that layer. Proteins which span the membrane can be found in either layer after freeze-fracture, depending on the properties of the protein. Which statement about the results of freeze-fracture studies are correct? 1 It provides evidence for the bilayer nature of membranes. 2 It provides evidence for the arrangement of proteins. 3 It shows that the proteins in the membrane do not contribute to its strength. A 1, 2 and 3 B 1 and 2 only C 2 and 3 only D 1 only 18 The stalk of a dandelion flower is a hollow tube. Pieces of the stalk are cut as shown and placed in sucrose solutions of different water potentials. hollow centre of stalk thick walled outer cells thin walled inner cells cuts Which diagram shows the piece that is placed in the sucrose solution with the highest water potential? C D A B

9 © UCLES 2016 9700/12/O/N/16 [Turn over 19 Three of the molecules found in prokaryotes are listed below. 1 phospholipid 2 protein 3 peptidoglycan Which of these molecules are found in prokaryotic cell surface membranes? A 1, 2 and 3 B 1 and 2 only C 1 and 3 only D 2 and 3 only 20 Which row correctly shows the behaviour of the nuclear envelope, the centrioles and the spindle during a stage of mitosis? stage of mitosis behaviour of organelle nuclear envelope centrioles spindle A prophase disappears replicate spindle microtubules begin to form B metaphase not present begin to move to poles of cell spindle microtubules fully formed C anaphase begins to reform at opposite poles of the cell some spindle microtubules shorten D telophase reforms one beside each nucleus spindle microtubules break down 21 Which group of cells can all divide by mitosis? A bacterial cells, cancer cells, stem cells B bacterial cells, lymphocytes, stem cells C cancer cells, lymphocytes, red blood cells D cancer cells, lymphocytes, stem cells 22 Which row represents the correct features of the nitrogenous base cytosine? has a single ring structure is a purine joins its complementary base by three hydrogen bonds A    key B    = true C    = false D   

10 © UCLES 2016 9700/12/O/N/16 23 Bacteria were grown in a medium containing 15N. After several generations, all of the DNA contained 15N. Some of these bacteria were transferred to a medium containing the common isotope of nitrogen, 14N. The bacteria were allowed to divide once. The DNA of some of these bacteria was extracted and analysed. This DNA was all hybrid DNA containing equal amounts of 14N and 15N. In another experiment, some bacteria from the medium with 15N were transferred into a medium of 14N. The bacteria were allowed to divide three times. The DNA of some of these bacteria was extracted and analysed. What is the composition of this DNA? 14N 14N / 15N 100 75 50 25 0 percentage of DNA A 14N 14N / 15N 100 75 50 25 0 percentage of DNA B 14N 14N / 15N 100 75 50 25 0 percentage of DNA C 14N 14N / 15N 100 75 50 25 0 percentage of DNA D 24 Which processes are involved in the mass movement of water through xylem vessels? 1 hydrogen bonding between water molecules 2 cohesion between water molecules 3 adhesion between water molecules and lignin A 1, 2 and 3 B 1 and 2 only C 1 and 3 only D 2 and 3 only

11 © UCLES 2016 9700/12/O/N/16 [Turn over 25 The diagrams show transverse sections of parts of a plant. 1 2 3 4 stem leaf In the transverse sections, which tissues transport most mineral ions? 1 2 3 4 A     key B     = yes C     = no D     26 Which changes to the water potential and the volume of liquid in the phloem occur when amino acids are transferred from leaves to be transported to a sink in the phloem sieve tube element? water potential in phloem sieve tube element volume of liquid in phloem sieve tube element A higher decreases B higher increases C lower decreases D lower increases 27 External factors change the rate of transpiration, which also affects the water potential gradient of cells in the leaf. Which combination of statements is correct? external factor changing the rate of transpiration water potential gradient in the leaf A decreasing temperature increases B decreasing wind speed increases C increasing relative humidity decreases D increasing sunlight decreases

12 © UCLES 2016 9700/12/O/N/16 28 The diagram shows a model to demonstrate mass flow. Y Z X W concentrated sucrose solution water dilute sucrose solution partially permeable membrane partially permeable membrane In a plant, what are the structures W, X, Y and Z and what is the direction of flow of solution along W? phloem xylem roots leaves direction of flow along W A W X Y Z from Z to Y B W X Z Y from Y to Z C X W Y Z from Y to Z D X W Z Y from Z to Y 29 Which blood vessels carry blood into the atria of the heart? A coronary artery and pulmonary artery B pulmonary artery and vena cava C pulmonary vein and vena cava D vena cava and coronary artery 30 Which statement describes the effect of increased carbon dioxide concentration on the oxygen dissociation curve of human haemoglobin and its significance? A The curve shifts to the left allowing haemoglobin to bind more oxygen in active tissues. B The curve shifts to the left allowing haemoglobin to offload more oxygen in active tissues. C The curve shifts to the right allowing haemoglobin to bind more oxygen in active tissues. D The curve shifts to the right allowing haemoglobin to offload more oxygen in active tissues. 31 What combines with haemoglobin to form carbaminohaemoglobin? A carbon dioxide B carbon monoxide C carbonic acid D hydrogencarbonate ions

13 © UCLES 2016 9700/12/O/N/16 [Turn over 32 The graph shows the pressure in three parts of the heart during one cycle. 0 0 0.2 0.4 0.6 0.8 pressure / kPa time / s left ventricle aorta left atrium key At 0.2 seconds, which part of the heart is responding to the excitatory stimulus? A D B C

14 © UCLES 2016 9700/12/O/N/16 33 The diagram shows part of the organisation of the circulation of a mammal. 1 2 3 4 aorta vena cava vena cava vena cava Where are both the blood pressure and speed of flow lowest? lowest blood pressure lowest speed of flow A 1 4 B 2 3 C 3 2 D 4 1 34 A student made some labelled drawings of lung tissue using the high-power lens of a light microscope, but did not label two airways, P and Q. The drawing of the lining layer of P showed both ciliated cells and goblet cells, whilst the drawing of the lining layer of Q showed ciliated cells but very few goblet cells. What are airways P and Q? airway P Q A bronchiole trachea B bronchiole bronchus C bronchus bronchiole D trachea bronchus

15 © UCLES 2016 9700/12/O/N/16 [Turn over 35 What maintains the steep concentration gradients needed for successful gas exchange in the lungs? 1 Air flow in the alveoli is in the opposite direction to blood flow in the capillaries. 2 Blood arrives in the lungs with a lower oxygen concentration and a higher carbon dioxide concentration than the air in the alveoli. 3 Blood is constantly flowing through and out of the lungs, bringing a fresh supply of red blood cells. A 1, 2 and 3 B 1 and 2 only C 1 and 3 only D 2 and 3 only 36 The photomicrograph shows part of the lung as seen using a light microscope. 1 2 3 Which row is correct for the features labelled 1, 2 and 3? 1 2 3 A alveolus bronchiole capillary B alveolus capillary bronchiole C bronchiole alveolus small artery D small artery alveolus bronchiole 37 Which disease is not likely to be passed directly from parents to child? A cholera B malaria C sickle cell anaemia D tuberculosis

16 Permission to reproduce items where third-party owned material protected by copyright is included has been sought and cleared where possible. Every reasonable effort has been made by the publisher (UCLES) to trace copyright holders, but if any items requiring clearance have unwittingly been included, the publisher will be pleased to make amends at the earliest possible opportunity. To avoid the issue of disclosure of answer-related information to candidates, all copyright acknowledgements are reproduced online in the Cambridge International Examinations Copyright Acknowledgements Booklet. This is produced for each series of examinations and is freely available to download at www.cie.org.uk after the live examination series. Cambridge International Examinations is part of the Cambridge Assessment Group. Cambridge Assessment is the brand name of University of Cambridge Local Examinations Syndicate (UCLES), which is itself a department of the University of Cambridge. © UCLES 2016 9700/12/O/N/16 38 Why is it difficult to control the spread of measles? 1 Global air travel for commerce and tourism has increased. 2 The virus that causes measles rapidly evolves resistance to antibiotics. 3 The virus that causes measles shows great antigenic variability. 4 Civil unrest and poverty result in overcrowded living conditions. A 1, 2 and 4 B 1, 2 and 3 C 1 and 4 only D 4 only 39 The following are all responses made by cells of the immune system to a pathogen. 1 mitosis 2 bind to infected cell 3 produce memory cells 4 secrete antibodies Which of the responses is correct for β-lymphocytes? A 1, 2, 3 and 4 B 1, 2 and 4 only C 1 and 3 only D 2, 3 and 4 only 40 What occurs when active immunity is artificially induced? A Non-self antibodies attack self antigens. B Non-self antigens attack self antibodies. C Self antibodies attack non-self antigens. D Self antigens attack non-self antibodies.

® IGCSE is the registered trademark of Cambridge International Examinations. This document consists of 2 printed pages. © UCLES 2016 [Turn over Cambridge International Examinations Cambridge International Advanced Subsidiary and Advanced Level BIOLOGY 9700/12 Paper 1 Multiple Choice October/November 2016 MARK SCHEME Maximum Mark: 40 Published This mark scheme is published as an aid to teachers and candidates, to indicate the requirements of the examination. It shows the basis on which Examiners were instructed to award marks. It does not indicate the details of the discussions that took place at an Examiners’ meeting before marking began, which would have considered the acceptability of alternative answers. Mark schemes should be read in conjunction with the question paper and the Principal Examiner Report for Teachers. Cambridge will not enter into discussions about these mark schemes. Cambridge is publishing the mark schemes for the October/November 2016 series for most Cambridge IGCSE®, Cambridge International A and AS Level components and some Cambridge O Level components.

Page 2 Mark Scheme Syllabus Paper Cambridge International AS/A Level – October/November 2016 9700 12 © UCLES 2016 Question Number Key Question Number Key 1 B 21 D 2 C 22 B 3 C 23 C 4 C 24 A 5 B 25 B 6 B 26 D 7 D 27 C 8 D 28 B 9 D 29 C 10 A 30 D 11 C 31 A 12 B 32 C 13 A 33 D 14 D 34 C 15 A 35 D 16 A 36 C 17 B 37 B 18 A 38 C 19 B 39 A 20 D 40 C