21.2· 11 questions · 11 marks · 13 min · 2018–2025· Multiple choice
Every Cambridge IGCSE Biology (9-1) Paper 2 question on biotechnology, laid out as 4 A4 pages with the mark scheme below. Nothing is left out. Free to read, no account.

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4 / 4Answers below. Sit the paper first if you are practising.
Pastlit
Biology (9-1) 0970 · Biotechnology — Paper 2
IGCSE · topical answer key — answer key (teacher use)
Question
Answer
Marks
| Question | Answer | Marks | From |
|---|---|---|---|
| 1 | B | 1 | 0970/21 May/June 2018 |
| 2 | A | 1 | 0970/21 May/June 2018 |
| 3 | B | 1 | 0970/22 Oct/Nov 2018 |
| 4 | B | 1 | 0970/21 May/June 2020 |
| 5 | C | 1 | 0970/22 May/June 2020 |
| 6 | A | 1 | 0970/22 Oct/Nov 2021 |
| 7 | B | 1 | 0970/22 Oct/Nov 2022 |
| 8 | C | 1 | 0970/22 May/June 2023 |
| 9 | D | 1 | 0970/22 Oct/Nov 2023 |
| 10 | C | 1 | 0970/22 Oct/Nov 2024 |
| 11 | D | 1 | 0970/22 May/June 2025 |
17 A student places two samples of crushed apple into two beakers, P and Q. The samples are of equal size. She adds 5 cm3 of pectinase solution to beaker P and 5 cm3 of water to beaker Q. After five minutes, she places the samples of crushed apple into two different filter funnels, and measures the volume of juice filtering through from each sample over a period of 10 minutes. Which graph shows her results? A B Q P total volume total volume of juice filtered P of juice filtered through through Q 00 00 time time C D P Q total volume total volume of juice filtered of juice filtered Q through P through 00 00 time time
1 marks
Answer: B
38 What does Penicillium need to grow in a fermenter? amino acids | carbohydrates oxygen v v v 0 0O WwW > v v x v x v x v v
1 marks
Answer: A
37 The diagram shows an industrial fermenter used to produce penicillin. X What is a function of the part labelled X? A add oxygen to the solution B maintain an even temperature throughout the solution C record the pH of the solution D sterilise the solution
1 marks
Answer: B
37 What is the role of anaerobic respiration in bread-making? A to produce alcohol to flavour the bread B to produce gas to make the bread rise C to release enough energy to bake the bread D to release enough lactic acid to kill the yeast
1 marks
Answer: B
39 Which is a reason for using bacteria in biotechnology? A Bacteria are found inside the human body. B Bacteria do not become resistant to antibiotics. C Bacteria can make complex molecules. D Bacteria reproduce slowly.
1 marks
Answer: C
38 What is the useful product of anaerobic respiration in the manufacture of bread? A carbon dioxide B ethanol C lactic acid D oxygen
1 marks
Answer: A
37 The graph shows how much penicillin is produced by the fungus Penicillium in a fermenter. It also shows the concentrations of sugars, ammonium ions and Penicillium. Penicillium uses sugars as a food source and needs ammonium ions. penicillin sugars concentration Penicillium ammonium ions time What does the graph show about the conditions needed for penicillin production? A A high concentration of ammonium ions must be maintained. B Most penicillin is produced when nutrient concentrations are low. C Penicillium cannot produce penicillin when its concentration decreases. D Sugars must be added constantly to the fermenter.
1 marks
Answer: B
40 Yeast carries out anaerobic respiration, making carbon dioxide and ethanol as end-products. Which end-products can be used to make biofuel and bread? biofuel bread A carbon dioxide carbon dioxide B carbon dioxide ethanol C ethanol carbon dioxide D ethanol ethanol
1 marks
Answer: C
23 Yeast cells can convert glucose into alcohol and carbon dioxide. Which statement about this process is correct? A The alcohol produced can be used to make bread rise. B The carbon dioxide produced can be burnt as a biofuel. C The yeast cells are using oxygen for this process. D The yeast cells are carrying out anaerobic respiration.
1 marks
Answer: D
40 The diagram shows some apparatus that was used to produce lactose-free milk. milk alginate beads containing X milk containing Y The alginate beads do not react with any of the substances. X is an enzyme that catalyses a reaction involving one of the substances found in milk. What are X and Y? X Y A amylase glucose B amylase starch C lactase glucose D lactase starch
1 marks
Answer: C
40 Which features make bacteria useful in biotechnology? 1 They are unaffected by antibiotics. 2 They do not contain plasmids. 3 They make complex molecules. 4 They reproduce rapidly. A 1 and 2 B 1 and 4 C 2 and 3 D 3 and 4
1 marks
Answer: D