TopicalBiology (9-1) 0970Biotechnology and genetic modificationBiotechnologyPaper 2

Biotechnology — Paper 2 · IGCSE Biology (9-1) 0970

21.2· 11 questions · 11 marks · 13 min · 2018–2025· Multiple choice

Every Cambridge IGCSE Biology (9-1) Paper 2 question on biotechnology, laid out as 4 A4 pages with the mark scheme below. Nothing is left out. Free to read, no account.

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Questions4 pages

Question 1: A student places two samples of crushed apple into two beakers, P and Q. The samples are of equal size. She adds 5 cm3 of pectinase solutio…Question 2: What does Penicillium need to grow in a fermenter?

amino acids | carbohydrates oxygen

v v v

0 0O WwW >

v v x
v x v
x v v1 / 4
Question 3: The diagram shows an industrial fermenter used to produce penicillin. X What is a function of the part labelled X? A add oxygen to the solu…Question 4: What is the role of anaerobic respiration in bread-making? A to produce alcohol to flavour the bread B to produce gas to make the bread ris…Question 5: Which is a reason for using bacteria in biotechnology? A Bacteria are found inside the human body. B Bacteria do not become resistant to an…Question 6: What is the useful product of anaerobic respiration in the manufacture of bread? A carbon dioxide B ethanol C lactic acid D oxygen2 / 4
Question 7: The graph shows how much penicillin is produced by the fungus Penicillium in a fermenter. It also shows the concentrations of sugars, ammon…Question 8: Yeast carries out anaerobic respiration, making carbon dioxide and ethanol as end-products. Which end-products can be used to make biofuel …Question 9: Yeast cells can convert glucose into alcohol and carbon dioxide. Which statement about this process is correct? A The alcohol produced can …3 / 4
Question 10: The diagram shows some apparatus that was used to produce lactose-free milk. milk alginate beads containing X milk containing Y The alginat…Question 11: Which features make bacteria useful in biotechnology? 1 They are unaffected by antibiotics. 2 They do not contain plasmids. 3 They make com…4 / 4

Mark scheme11 answers

Answers below. Sit the paper first if you are practising.

Pastlit

Biology (9-1) 0970 · Biotechnology — Paper 2

IGCSE · topical answer key — answer key (teacher use)

Question

Answer

Marks

1B1
2A1
3B1
4B1
5C1
6A1
7B1
8C1
9D1
10C1
11D1
1 / 1
QuestionAnswerMarksFrom
1B10970/21 May/June 2018
2A10970/21 May/June 2018
3B10970/22 Oct/Nov 2018
4B10970/21 May/June 2020
5C10970/22 May/June 2020
6A10970/22 Oct/Nov 2021
7B10970/22 Oct/Nov 2022
8C10970/22 May/June 2023
9D10970/22 Oct/Nov 2023
10C10970/22 Oct/Nov 2024
11D10970/22 May/June 2025

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Questions as text

Q1 · A student places two samples of crushed apple into two beakers, P and Q 0970/21 May/June 2018

17 A student places two samples of crushed apple into two beakers, P and Q. The samples are of equal size. She adds 5 cm3 of pectinase solution to beaker P and 5 cm3 of water to beaker Q. After five minutes, she places the samples of crushed apple into two different filter funnels, and measures the volume of juice filtering through from each sample over a period of 10 minutes. Which graph shows her results? A B Q P total volume total volume of juice filtered P of juice filtered through through Q 00 00 time time C D P Q total volume total volume of juice filtered of juice filtered Q through P through 00 00 time time

1 marks

Answer: B

This question in 0970/21 May/June 2018

Q2 · What does Penicillium need to grow in a fermenter? 0970/21 May/June 2018

38 What does Penicillium need to grow in a fermenter? amino acids | carbohydrates oxygen v v v 0 0O WwW > v v x v x v x v v

1 marks

Answer: A

This question in 0970/21 May/June 2018

Q3 · The diagram shows an industrial fermenter used to produce penicillin 0970/22 Oct/Nov 2018

37 The diagram shows an industrial fermenter used to produce penicillin. X What is a function of the part labelled X? A add oxygen to the solution B maintain an even temperature throughout the solution C record the pH of the solution D sterilise the solution

1 marks

Answer: B

This question in 0970/22 Oct/Nov 2018

Q4 · What is the role of anaerobic respiration in bread-making? 0970/21 May/June 2020

37 What is the role of anaerobic respiration in bread-making? A to produce alcohol to flavour the bread B to produce gas to make the bread rise C to release enough energy to bake the bread D to release enough lactic acid to kill the yeast

1 marks

Answer: B

This question in 0970/21 May/June 2020

Q5 · Which is a reason for using bacteria in biotechnology? 0970/22 May/June 2020

39 Which is a reason for using bacteria in biotechnology? A Bacteria are found inside the human body. B Bacteria do not become resistant to antibiotics. C Bacteria can make complex molecules. D Bacteria reproduce slowly.

1 marks

Answer: C

This question in 0970/22 May/June 2020

Q6 · What is the useful product of anaerobic respiration in the manufacture of bread? 0970/22 Oct/Nov 2021

38 What is the useful product of anaerobic respiration in the manufacture of bread? A carbon dioxide B ethanol C lactic acid D oxygen

1 marks

Answer: A

This question in 0970/22 Oct/Nov 2021

Q7 · The graph shows how much penicillin is produced by the fungus Penicillium in a fermenter 0970/22 Oct/Nov 2022

37 The graph shows how much penicillin is produced by the fungus Penicillium in a fermenter. It also shows the concentrations of sugars, ammonium ions and Penicillium. Penicillium uses sugars as a food source and needs ammonium ions. penicillin sugars concentration Penicillium ammonium ions time What does the graph show about the conditions needed for penicillin production? A A high concentration of ammonium ions must be maintained. B Most penicillin is produced when nutrient concentrations are low. C Penicillium cannot produce penicillin when its concentration decreases. D Sugars must be added constantly to the fermenter.

1 marks

Answer: B

This question in 0970/22 Oct/Nov 2022

Q8 · Yeast carries out anaerobic respiration, making carbon dioxide and ethanol as end-products 0970/22 May/June 2023

40 Yeast carries out anaerobic respiration, making carbon dioxide and ethanol as end-products. Which end-products can be used to make biofuel and bread? biofuel bread A carbon dioxide carbon dioxide B carbon dioxide ethanol C ethanol carbon dioxide D ethanol ethanol

1 marks

Answer: C

This question in 0970/22 May/June 2023

Q9 · Yeast cells can convert glucose into alcohol and carbon dioxide 0970/22 Oct/Nov 2023

23 Yeast cells can convert glucose into alcohol and carbon dioxide. Which statement about this process is correct? A The alcohol produced can be used to make bread rise. B The carbon dioxide produced can be burnt as a biofuel. C The yeast cells are using oxygen for this process. D The yeast cells are carrying out anaerobic respiration.

1 marks

Answer: D

This question in 0970/22 Oct/Nov 2023

Q10 · The diagram shows some apparatus that was used to produce lactose-free milk 0970/22 Oct/Nov 2024

40 The diagram shows some apparatus that was used to produce lactose-free milk. milk alginate beads containing X milk containing Y The alginate beads do not react with any of the substances. X is an enzyme that catalyses a reaction involving one of the substances found in milk. What are X and Y? X Y A amylase glucose B amylase starch C lactase glucose D lactase starch

1 marks

Answer: C

This question in 0970/22 Oct/Nov 2024

Q11 · Which features make bacteria useful in biotechnology? 0970/22 May/June 2025

40 Which features make bacteria useful in biotechnology? 1 They are unaffected by antibiotics. 2 They do not contain plasmids. 3 They make complex molecules. 4 They reproduce rapidly. A 1 and 2 B 1 and 4 C 2 and 3 D 3 and 4

1 marks

Answer: D

This question in 0970/22 May/June 2025