Cambridge A Level Biology 9700 — 2014 Oct/Nov Paper 1 · Variant 2
9700/12/O/N/14 · 40 questions · 40 marks · ≈45 min
The question paper and its mark scheme, free to read here and free to download. This is Cambridge’s own paper, exactly as it was sat.
Question paper16 pages
















Mark scheme2 pages
Answers below. Sit the paper first if you are practising.


Questions as text
Q1 · Which combination of lenses for a light microscope will give the greatest magnification?
1 Which combination of lenses for a light microscope will give the greatest magnification? eyepiece lens objective lens A ×5 ×100 B ×10 ×40 C ×15 ×40 D ×15 ×100
Mark scheme: D
Q2 · Which steps are needed to find the actual width of a xylem vessel viewed in transverse…
2 Which steps are needed to find the actual width of a xylem vessel viewed in transverse section using a ×10 objective lens? 1 Convert from mm to µm by multiplying by 10–3. 2 Calibrate the eyepiece graticule using a stage micrometer on ×4 objective lens. 3 Measure the width of the xylem vessel using an eyepiece graticule. 4 Multiply the number of eyepiece graticule units by the calibration of the eyepiece graticule. A 1, 2, 3 and 4 B 2, 3 and 4 only C 1 and 2 only D 3 and 4 only
Mark scheme: D
Q3 · The statements are all descriptions of cell structures
3 The statements are all descriptions of cell structures. 1 surrounded by a single membrane and enclosing a large fluid-filled space 2 surrounded by a single membrane and enclosing inactivated enzymes 3 formed by two membranes enclosing a matrix, the inner membrane is folded 4 formed by a membrane that has flattened sacs and tubular structures inter-connected throughout the cell 5 formed of nucleic acid and protein attached to membranes or free in the cytoplasm Which row shows the typical cell in which these cell structures are found? plant cell animal cell A 1, 2, 3 and 4 1, 2, 3 and 5 B 1, 3, 4 and 5 2, 3, 4 and 5 C 2, 4 and 5 1, 4 and 5 D 3, 4 and 5 only 2, 3 and 5 only
Mark scheme: B
More questions on Cells as the basic units of living organisms
Q4 · Which structures are found in both typical eukaryotic cells and typical prokaryotic cells?
4 Which structures are found in both typical eukaryotic cells and typical prokaryotic cells? 1 70S ribsomes 2 80S ribosomes 3 circular DNA A 1, 2 and 3 B 1 and 3 only C 1 only D 2 only
Mark scheme: B
More questions on Cells as the basic units of living organisms
Q5 · Which sequence shows some of the stages in the production and secretion of an enzyme?
5 Which sequence shows some of the stages in the production and secretion of an enzyme? A Golgi apparatus → ribosome → rough endoplasmic reticulum → mRNA B mRNA → smooth endoplasmic reticulum → Golgi apparatus → vesicle C ribosome → rough endoplasmic reticulum → vesicle → Golgi apparatus D smooth endoplasmic reticulum → mRNA → vesicle → ribosome
Mark scheme: C
More questions on Cells as the basic units of living organisms
Q6 · Which plan diagram of a transverse section of a dicotyledonous leaf correctly shows the…
6 Which plan diagram of a transverse section of a dicotyledonous leaf correctly shows the position of xylem and phloem in a palisade mesophyll layer that is twice as thick as the spongy mesophyll layer? A B xylem phloem phloem xylem C D xylem phloem phloem xylem
Mark scheme: C
Q7 · Proteases are a group of enzymes that digest proteins
7 Proteases are a group of enzymes that digest proteins. Which statement about proteases is correct? A A bacterial protease that is secreted from a disease-causing bacterium could act as an antigen and cause the production of an antibody. B Bacterial proteases are important in the nitrogen cycle as they are able to catalyse the breakdown of the organic nitrogen molecules nucleotides and urea. C Non-competitive inhibition of a protease that has an optimum pH of pH2 can be overcome by increasing the substrate concentration and increasing the pH. D Water molecules are required when peptide bonds that link the monomers together are broken by the action of proteases in condensation reactions.
Mark scheme: A
Q8 · Two disaccharides are maltose and sucrose
8 Two disaccharides are maltose and sucrose. Maltose is formed from two molecules of glucose, whilst sucrose is formed from fructose and glucose. Which row shows the molecular formulae of the two disaccharides? maltose sucrose A C12H22O11 C12H22O11 B C12H22O11 C12H24O12 C C12H24O12 C12H22O11 D C12H24O12 C12H24O12
Mark scheme: A
Q9 · The diagram shows a carbohydrate molecule
9 The diagram shows a carbohydrate molecule. CH2OH C O OH HO O HO CH2OH CH2 CH2OH C O C O C O OH OH OH O O HO OH HO HO HO Of which polymers could this be a part? A amylopectin and cellulose B amylose and starch C glycogen and amylose D starch and glycogen
Mark scheme: D
Q10 · Which of the bonds stabilising the tertiary structure of a protein would be least likely…
10 Which of the bonds stabilising the tertiary structure of a protein would be least likely to break when the temperature is increased above the optimum for the protein? A disulfide B hydrogen C hydrophobic D ionic
Mark scheme: A
Q11 · The diagram shows the structure of four amino acids in solution
11 The diagram shows the structure of four amino acids in solution. COO– COO– H C H3N+ CH2 CH2 CH2 CH2 C NH3 + NH3 + H H glycine lysine COO– O COO– CH3 C C CH2 C NH3 + O– NH3 + H H alanine aspartate Which amino acids have no overall charge? A alanine and aspartate B alanine and glycine C aspartate and lysine D glycine and lysine
Mark scheme: B
Q12 · The statements are about the properties of water
12 The statements are about the properties of water. 1 It requires a lot of heat to evaporate. 2 It retains a lot of heat. 3 It is able to form hydrogen bonds with other water molecules. 4 It is able to form hydrogen bonds with other polar and non-polar molecules. Which properties are important in transpiring plants to prevent enzymes in leaves from being denatured? A 1 only B 1 and 2 only C 2 and 3 only D 1, 2, 3 and 4
Mark scheme: A
Q13 · Which words from the table correctly complete the paragraph about enzymes?
13 Which words from the table correctly complete the paragraph about enzymes? When the pH of an environment is decreased below an enzyme’s optimum pH, ……1…… bonds between adjacent ……2…… groups, holding the ……3…… structure, are disrupted. 1 2 3 A hydrogen and ionic R tertiary B hydrogen hydroxyl secondary C ionic and peptide R primary and tertiary D peptide amine primary
Mark scheme: A
Q14 · Lipase is a digestive enzyme produced by the pancreas that catalyses the hydrolysis of…
14 Lipase is a digestive enzyme produced by the pancreas that catalyses the hydrolysis of dietary lipids. The table shows how the pH of a liquid food containing a high proportion of lipids decreases over time. 8 pH of a 7 liquid food 6 0 5 10 15 20 25 30 35 40 45 50 55 60 time / min Which statements are possible explanations of the results of the experiment between 50 and 60 minutes? 1 Enzyme concentration becomes the limiting factor. 2 Substrate concentration becomes the limiting factor. 3 All the enzyme active sites are saturated. 4 Denaturation of the enzyme by the products. 5 Products are acting as competitive inhibitors. A 1, 2 and 3 B 1, 4 and 5 C 2, 3 and 4 D 2, 4 and 5
Mark scheme: D
Q15 · The AIDS virus produces a long polypeptide that is hydrolysed by a protease enzyme…
15 The AIDS virus produces a long polypeptide that is hydrolysed by a protease enzyme, producing several smaller peptides. This viral protease is the target of anti-AIDS drugs. Which feature is essential for the success of these drugs? A a complex structure that inhibits many types of viral enzyme B a molecule containing a heavy metal atom that is a non-competitive inhibitor of enzymes C a protein that can act as a competitive inhibitor of protease enzymes D a specific structure that inhibits only viral protease
Mark scheme: D
Q16 · What is the width of the cell surface membrane?
16 What is the width of the cell surface membrane? A 0.5 – 1.0 nm B 5 – 10 nm C 50 – 100 nm D 0.5 – 1 µm
Mark scheme: B
Q17 · Some functions of molecules found in cell surface membranes are listed
17 Some functions of molecules found in cell surface membranes are listed. 1 cell recognition 2 ion transport 3 maintaining fluidity 4 mechanical stability Which are functions of cholesterol? A 1 and 2 B 1 and 4 C 2 and 3 D 3 and 4
Mark scheme: D
Q18 · The enzyme lactase is found in the membranes of epithelial cells lining the small…
18 The enzyme lactase is found in the membranes of epithelial cells lining the small intestine. The enzyme is formed by a single polypeptide that folds to give three regions. • an active site with the free amino group outside the cell • a short section inside the membrane • a short section inside the cell What type of amino acid would be found in each of the three regions? inside the inside the outside cell membrane cell A hydrophilic hydrophobic hydrophilic B hydrophilic hydrophobic hydrophobic C hydrophobic hydrophilic hydrophobic D hydrophobic hydrophobic hydrophilic
Mark scheme: A
Q19 · A student examined the cells in the growing region (meristem) of an onion root and…
19 A student examined the cells in the growing region (meristem) of an onion root and obtained the data below. stage number of cells interphase 886 prophase 73 metaphase 16 anaphase 14 telophase 11 What percentage of cells contain chromosomes that appear as two chromatids? A 97.5 % B 95.9 % C 8.9 % D 7.3 %
Mark scheme: C
Q20 · Which definitions of diploid and haploid are true for typical eukaryotic cells?
20 Which definitions of diploid and haploid are true for typical eukaryotic cells? 1 diploid – any cell with an even number of homologous chromosomes 2 diploid – any cell with two copies of each homologous chromosome 3 haploid – any cell with half the diploid number of homologous chromosomes 4 haploid – any cell with one copy of each homologous chromosome A 1 and 3 B 1 and 4 C 2 and 3 D 2 and 4
Mark scheme: D
More questions on Replication and division of nuclei and cells
Q21 · Which type of sugar and types of bonds are found in a DNA molecule?
21 Which type of sugar and types of bonds are found in a DNA molecule? type of sugar types of bonds A non-reducing hydrogen and ionic B non-reducing hydrogen and peptide C reducing covalent and hydrogen D reducing hydrogen and peptide
Mark scheme: C
More questions on Structure of nucleic acids and replication of DNA
Q22 · Which nucleic acid bases are purines?
22 Which nucleic acid bases are purines? A adenine and cytosine B cytosine and thymine C guanine and adenine D uracil and cytosine
Mark scheme: C
More questions on Structure of nucleic acids and replication of DNA
Q23 · A short piece of DNA 15 base pairs long was analysed to find the number of nucleotide…
23 A short piece of DNA 15 base pairs long was analysed to find the number of nucleotide bases in each of the polynucleotide strands. Some of the results are shown below. number of nucleotide bases A C G T strand 1 6 3 strand 2 4 How many nucleotides containing guanine were present in strand 1? A 2 B 3 C 4 D 6
Mark scheme: A
More questions on Structure of nucleic acids and replication of DNA
Q24 · The diagram shows a transverse section of a stem
24 The diagram shows a transverse section of a stem. Which area is the phloem? A D C B
Mark scheme: B
Q25 · A leafy shoot was attached to a potometer and exposed to a variety of conditions
25 A leafy shoot was attached to a potometer and exposed to a variety of conditions. The time taken for the meniscus to move 50 mm along the capillary tubing was recorded for each set of conditions. time taken to temperature air humidity move 50 mm / °C movement / s 20 dry still 125 20 dry moving 71 30 dry still 40 20 humid still 166 20 humid moving 83 30 humid still 55 Which shows the slowest and fastest rates of water uptake? slowest fastest / mm s–1 / mm s–1 A 0.3 1.3 B 0.3 3.3 C 3.3 0.3 D 1.3 0.8
Mark scheme: A
Q26 · The diagram shows four plant cells
26 The diagram shows four plant cells. 1 4 2 3 In which direction could there be net movement of water by osmosis? A 1 to 2 and 1 to 4 B 1 to 3 and 1 to 2 C 2 to 1 and 1 to 4 D 4 to 1 and 2 to 3
Mark scheme: A
Q27 · How will the root pressure in a plant be affected by waterlogged soil?
27 How will the root pressure in a plant be affected by waterlogged soil? A It will not be affected. B It will decrease due to a lack of oxygen in the soil. C It will increase due to a lower water potential in the soil. D It will increase due to a higher rate of transpiration.
Mark scheme: B
Q28 · The diagram shows the relationship between phloem sieve tube elements, xylem vessel…
28 The diagram shows the relationship between phloem sieve tube elements, xylem vessel elements and companion cells. 1 2 3 4 5 Which is correct? 1 2 3 4 5 A companion endoplasmic phloem sieve nucleus absent xylem vessel cells reticulum tube elements elements B companion nucleus phloem sieve cytoplasm xylem vessel cells tube elements elements C phloem sieve mitochondria companion nucleus xylem vessel tube elements cells elements D xylem vessel cytoplasm phloem sieve vacuole companion elements absent tube elements cells
Mark scheme: A
Q29 · Small arteries (arterioles) are found near tissues
29 Small arteries (arterioles) are found near tissues. One function of an arteriole is to increase or decrease the flow of blood to the tissues. Which tissues would be present in an arteriole wall to allow this function? 1 collagen 2 elastic 3 muscle A 1, 2 and 3 B 1 and 3 only C 2 only D 3 only
Mark scheme: D
Q30 · The graph shows a dissociation curve for haemoglobin
30 The graph shows a dissociation curve for haemoglobin. y-axis x-axis Which are suitable labels for the x-axis and the y-axis? x-axis y-axis A partial pressure of carbon dioxide percentage of haemoglobin B partial pressure of oxygen percentage saturation of haemoglobin with oxygen C percentage of haemoglobin partial pressure of oxygen D percentage saturation of haemoglobin partial pressure of carbon dioxide with oxygen
Mark scheme: B
Q31 · One type of congenital heart defect is called atrial septal defect (ASD) where the left…
31 One type of congenital heart defect is called atrial septal defect (ASD) where the left and right atria are not completely separated. ASD usually results in blood moving from the left atrium into the right atrium. This will cause increased blood pressure in the right atrium and decreased blood pressure in the left atrium. Which row describes the other effects of ASD on blood pressure and oxygenation? blood pressure in blood pressure % oxygenation of blood pulmonary artery in aorta in pulmonary artery A decreased increased decreased B decreased increased increased C increased decreased decreased D increased decreased increased
Mark scheme: D
Q32 · Which are effects on the body of carbon monoxide in tobacco smoke?
32 Which are effects on the body of carbon monoxide in tobacco smoke? 1 It reduces the amount of haemoglobin available to transport oxygen. 2 It reduces the rate of diffusion of oxygen from the alveoli into the blood. 3 It inhibits the release of oxygen from haemoglobin. A 1, 2 and 3 B 2 and 3 only C 1 only D 3 only
Mark scheme: C
Q33 · The graph shows the volume of air breathed out quickly and with force, following a deep…
33 The graph shows the volume of air breathed out quickly and with force, following a deep breath in, for three different people, X, Y and Z. 5 X 4 Y 3 volume / dm3 2 Z 1 0 0 1 2 3 4 5 time / s What is a possible explanation for the differences in the volume of air breathed out by these people shown? chronic bronchitis emphysema normal lung function A X Z Y B Y X Z C Y Z X D Z Y X
Mark scheme: C
Q34 · Which flow diagram correctly describes the effect of tar entering the lungs?
34 Which flow diagram correctly describes the effect of tar entering the lungs? carcinogens A uncontrolled → mass of cells come into contact → mutation occurs → cell division produced with DNA mucus goblet cells phagocytes B accumulates causes irritation secrete more → → attracted by → causing and coughing mucus inflammation infection goblet cells phagocytes C mass of cells secrete more → mutation occurs → attracted by → produced mucus inflammation phagocytes D uncontrolled elastase destroys attracted by → mutation occurs → → cell division the alveolar walls inflammation
Mark scheme: A
Q35 · Which statements about people infected with HIV / AIDS are correct?
35 Which statements about people infected with HIV / AIDS are correct? 1 They can be treated and completely cured. 2 They may live for many years after infection. 3 They will have symptoms. A 1, 2 and 3 B 1 and 3 only C 2 only D 3 only
Mark scheme: C
Q36 · The statements refer to polio and tuberculosis (TB)
36 The statements refer to polio and tuberculosis (TB). 1 Macrophages present antigens in vaccines to stimulate an immune response. 2 The TB antigens necessary to produce an immune response are proteins which would be digested in the stomach and small intestine. 3 There are no B-lymphocytes and T-lymphocytes in the stomach. Which explains why polio vaccine can be taken by mouth but TB vaccine has to be injected? A 1, 2 and 3 B 1 and 2 only C 1 and 3 only D 2 only
Mark scheme: D
Q37 · Which explains why sickle cell anaemia has not been eradicated?
37 Which explains why sickle cell anaemia has not been eradicated? A Identification of carriers is too difficult as they show no symptoms. B Sickle cell anaemia gives the individual some resistance against malaria. C Sickle cell anaemia is an inherited disease, so vaccination is not possible. D The gene responsible for sickle cell anaemia has a high rate of mutation.
Mark scheme: C
Q38 · Which method of gaining immunity can be described as natural active immunity?
38 Which method of gaining immunity can be described as natural active immunity? A feeding on colostrum B inhaling the chicken pox virus C injection with antibodies D through the placenta
Mark scheme: B
Q39 · Which stage of energy transfer has the lowest efficiency?
39 Which stage of energy transfer has the lowest efficiency? A sunlight → producer B producer → primary consumer C primary consumer → secondary consumer D secondary consumer → tertiary consumer
Mark scheme: A
Q40 · The diagram shows a simplified nitrogen cycle
40 The diagram shows a simplified nitrogen cycle. During which stage does decomposition take place? nitrogen in atmosphere A plant animal proteins proteins B ammonium compounds nitrites nitrates C D
Mark scheme: B
What was in this paper
The subtopics covered by these 40 questions, and how many questions each got. Open one in a new tab to see every Cambridge question on it.
3Fluid mosaic membranes3Structure of nucleic acids and replication of DNA3Structure of transport tissues3Antibodies and vaccination2Carbohydrates and lipids2Energy2Factors that affect enzyme action2Proteins2The gas exchange system2The microscope in cell studies2Transport mechanisms2Transport of oxygen and carbon dioxide2Antibiotics1Chromosome behaviour in mitosis1Evolution1Infectious diseases1Mode of action of enzymes1Movement into and out of cells1Replication and division of nuclei and cells1The circulatory system1The heart1Water1What you needed in this session
Cambridge’s own grade thresholds for 2014 Oct/Nov, Paper 1 · Variant 2. A higher threshold means an easier paper — the bar moves with how the cohort did.