Cambridge A Level Biology 9700 — 2013 May/June Paper 1 · Variant 2
9700/12/M/J/13 · 40 questions · 40 marks · ≈45 min
The question paper and its mark scheme, free to read here and free to download. This is Cambridge’s own paper, exactly as it was sat.
Question paper20 pages




















Mark scheme2 pages
Answers below. Sit the paper first if you are practising.


Questions as text
Q1 · A light microscope is used to observe two membranes that are 200 nm apart
1 A light microscope is used to observe two membranes that are 200 nm apart. How far apart are the membranes when the objective lens is changed from low power (×40) to high power (×400)? A 2 µm B 20 µm C 200 nm D 2000 nm
Mark scheme: C
Q2 · The electronmicrograph is of a chloroplast
2 The electronmicrograph is of a chloroplast. The length of the chloroplast along the line shown is 80 mm. The actual length of the chloroplast is 10 µm. What is the magnification of the chloroplast? A ×8 × 102 B ×8 × 103 C ×8 × 104 D ×8 × 106
Mark scheme: B
Q3 · The diagram below is drawn from an electronmicrograph of an animal cell
3 The diagram below is drawn from an electronmicrograph of an animal cell. Which represents the same cell, seen under a light microscope at ×400 magnification?
Mark scheme: C
Q4 · Which features enable an organism to be identified as a prokaryote?
4 Which features enable an organism to be identified as a prokaryote? 1 cell wall 2 circular DNA 3 nucleus 4 ribosomes A 2 only B 3 only C 1 and 4 only D 2 and 4 only
Mark scheme: A
More questions on Cells as the basic units of living organisms
Q5 · The diagram shows an electronmicrograph of a typical animal cell
5 The diagram shows an electronmicrograph of a typical animal cell. X What is the function of the membrane system labelled X? A lipid synthesis B lipid synthesis and transport C protein synthesis D protein synthesis and transport
Mark scheme: D
More questions on Cells as the basic units of living organisms
Q6 · An animal cell and a plant cell are placed in distilled water
6 An animal cell and a plant cell are placed in distilled water. The animal cell swells and bursts, while the plant cell swells but does not burst. What accounts for this difference? A Animal cells have no cell wall. B Animal cells have no vacuole. C Plant cell surface membranes are partially permeable. D Plant cell walls are freely permeable.
Mark scheme: A
Q7 · The R groups (side chains) of amino acids can be hydrophobic, hydrophilic, acidic or basic
7 The R groups (side chains) of amino acids can be hydrophobic, hydrophilic, acidic or basic. The diagram shows four different amino acid R groups. COOH NH2 OH CH3 CH2 (CH2)4 CH2 Ala Asp Lys Ser Which row is correct for these amino acid R groups? hydrophobic hydrophilic acidic basic A Ala Ser Asp Lys B Ala Ser Lys Asp C Asp Lys Ser Ala D Asp Ser Lys Ala
Mark scheme: A
Q8 · Which describes the emulsion test for the presence of lipids?
8 Which describes the emulsion test for the presence of lipids? A Add ethanol and shake. B Add ethanol, pour into water and shake. C Add water and shake. D Add water, pour into ethanol and shake.
Mark scheme: B
Q9 · The structural formula of a carbohydrate molecule can be shown as: H C O H C OH HO C H H…
9 The structural formula of a carbohydrate molecule can be shown as: H C O H C OH HO C H H C OH H C OH CH2OH Which of the molecules could be represented by this formula? 1 ribose 2 β-glucose 3 sucrose A 1 only B 2 only C 1 and 2 only D 2 and 3 only
Mark scheme: B
Q10 · The diagram shows part of a molecule of glycogen
10 The diagram shows part of a molecule of glycogen. How does the molecule differ from a molecule of amylose? A Amylose has only α-1,4 glycosidic bonds. B Amylose has only β-1,4 glycosidic bonds. C Amylose has α-1,6 glycosidic bonds with 1,4 linkages forming branches. D Amylose has β-1,4 glycosidic bonds with 1,6 linkages forming branches.
Mark scheme: A
Q11 · Which molecules contain a carboxyl group?
11 Which molecules contain a carboxyl group? 1 amino acid 2 glycerol 3 saturated fatty acid 4 unsaturated fatty acid A 1 and 2 only B 3 and 4 only C 1, 3 and 4 only D 2, 3 and 4 only
Mark scheme: C
Q12 · The diameter of some atoms when they form bonds are given in the table
12 The diameter of some atoms when they form bonds are given in the table. single bond double bond atom / nm / nm H 0.060 – O 0.132 0.110 N 0.140 0.120 C 0.154 0.134 The approximate length of the amino acid shown below was estimated using the figures in the table. R O H N C C O H H H 0.7 nm What would be the approximate length of a dipeptide formed using this amino acid? A 0.9 nm B 1.2 nm C 1.4 nm D 1.7 nm
Mark scheme: B
Q13 · Which feature of water is least likely to affect the life of an animal in a tropical rain…
13 Which feature of water is least likely to affect the life of an animal in a tropical rain forest? A adhesion with other molecules B cohesion between water molecules C low viscosity D maximum density at 4 °C
Mark scheme: D
Q14 · Two pairs of students each counted the number of bubbles of oxygen given off in a minute…
14 Two pairs of students each counted the number of bubbles of oxygen given off in a minute when investigating the effect of catalase from plant tissue on hydrogen peroxide. Each pair repeated the experiment five times and calculated the mean number of bubbles per minute. Which have correctly calculated the mean? raw data / number of bubbles minute-1 mean / number of student bubbles minute-1 1 8 10 11 9 8 9.2 2 8 10 11 9 8 9 3 21 18 6 17 19 16.2 4 21 18 6 17 19 18.8 A 1, 3 and 4 only B 1 and 4 only C 2 only D 3 only
Mark scheme: C
Q15 · When investigating the rate of reaction of the enzyme lipase on the hydrolysis of…
15 When investigating the rate of reaction of the enzyme lipase on the hydrolysis of triglycerides, the pH must be maintained at an optimum to prevent the lipase denaturing. What is the reason for this? A The addition of water molecules produced by hydrolysis increases pH. B The products of hydrolysis decrease the pH. C The products of hydrolysis increase the pH. D The removal of water molecules used in hydrolysis decreases pH.
Mark scheme: B
Q16 · Which of the following can increase the fluidity of the cell surface membrane?
16 Which of the following can increase the fluidity of the cell surface membrane? 1 single bonds between carbon atoms in the fatty acid chains 2 cholesterol 3 fatty acids having longer chains A 1, 2 and 3 B 1 and 3 only C 2 and 3 only D 2 only
Mark scheme: D
Q17 · The diagram shows a cell that produces protease enzymes
17 The diagram shows a cell that produces protease enzymes. Which row is correct? enzymes released by ATP needed A endocytosis no B endocytosis yes C exocytosis no D exocytosis yes
Mark scheme: D
Q18 · The diagram shows two identical plant cells
18 The diagram shows two identical plant cells. One plant cell is put into a solution with a water potential less negative than the cell contents. The other is put into a solution with a water potential more negative than the cell contents. What will happen to the appearance of each cell? water potential of solution water potential of solution surrounding cell less surrounding cell more negative than cell contents negative than cell contents A B C D
Mark scheme: C
Q19 · Chromosome telomeres are essential for DNA replication and are not completely replaced…
19 Chromosome telomeres are essential for DNA replication and are not completely replaced during mitosis. A substance X is known that completely replaces telomeres during mitosis. What will be the effect of growing cells with and without substance X? with substance X without substance X A cells divide continually cell division eventually slows and stops B cells divide more rapidly cells divide continually C cell division eventually slows cell division stops immediately and stops D cell division stops immediately cells divide continually
Mark scheme: A
More questions on Replication and division of nuclei and cells
Q20 · The diagram shows a cell nucleus in prophase of mitosis
20 The diagram shows a cell nucleus in prophase of mitosis. Which statement describes the chromosomes found in each daughter nucleus immediately following division of this cell by mitosis? A 8 chromosomes, each consisting of 4 chromatids B 8 chromosomes, each containing 1 molecule of DNA C 4 chromosomes, each consisting of 4 chromatids D 16 chromosomes, each containing 1 molecule of DNA
Mark scheme: B
Q21 · Which features of mitosis ensure that the genetic constitution of the cell is maintained?
21 Which features of mitosis ensure that the genetic constitution of the cell is maintained? 1 the position of the chromosomes on the equator of the spindle 2 the longitudinal division of the centromeres 3 the DNA of the parent cells replicates before mitosis begins 4 the pulling apart of the chromatids to opposite poles A 1, 2 and 3 only B 1, 2 and 4 only C 2, 3 and 4 only D 1, 2, 3 and 4
Mark scheme: C
More questions on Replication and division of nuclei and cells
Q22 · What is the maximum number of hydrogen bonds in a length of DNA containing 700…
22 What is the maximum number of hydrogen bonds in a length of DNA containing 700 nucleotides? A 350 B 700 C 1050 D 2100
Mark scheme: C
More questions on Structure of nucleic acids and replication of DNA
Q23 · Which type of molecule is the end product of translation?
23 Which type of molecule is the end product of translation? A amino acid B mRNA C polypeptide D tRNA
Mark scheme: C
Q24 · A polypeptide molecule contains the amino acid sequence: glycine – leucine – lysine…
24 A polypeptide molecule contains the amino acid sequence: glycine – leucine – lysine – valine. The table shows DNA codes for these amino acids. glycine leucine lysine valine CCC GAA TTT CAA Which tRNA anticodons are needed for the synthesis of this polypeptide? A CCC GAA TTT CAA B CCC GAA UUU CAA C GGG CUU AAA GUU D GGG CUU UUU GUU
Mark scheme: B
Q25 · Which plant cells do not contain a nucleus?
25 Which plant cells do not contain a nucleus? 1 companion cells 2 sieve tube elements 3 xylem vessel elements A 1 and 2 only B 1 and 3 only C 2 and 3 only D 3 only
Mark scheme: C
Q26 · The diagram shows three xerophytic leaves of the same type in three different conditions…
26 The diagram shows three xerophytic leaves of the same type in three different conditions, P, R and S. layer X X X P R S Which description of the water potential of the cells in layer X is correct? water potential of cells in layer X P R S A less negative than R and S more negative than P and S more negative than P B less negative than S more negative than P less negative than P C more negative than R less negative than S less negative than R D more negative than R and S less negative than P more negative than P
Mark scheme: A
Q27 · The diagram shows how sucrose is loaded into a sieve tube element
27 The diagram shows how sucrose is loaded into a sieve tube element. companion cell sieve tube element 1 hydrogen ions move out of the companion cell 3 sucrose molecules move into sucrose molecules move into sucrose molecules move into the sieve tube element the sieve tube element the sieve tube element hydrogen ions move into the companion cell 2 sucrose molecules move into the companion cell What type of transport is used to move the substances in steps 1, 2 and 3? 1 2 3 A active transport active transport diffusion B active transport facilitated diffusion diffusion C facilitated diffusion active transport active transport D facilitated diffusion facilitated diffusion active transport
Mark scheme: B
Q28 · Halophytes are plants that can survive in regions where they are regularly exposed to sea…
28 Halophytes are plants that can survive in regions where they are regularly exposed to sea water. Sea water has a water potential of approximately –2500 kPa. Which adaptation would you expect halophytes to show? A root hair cells with a very high water potential B root hair cells which accumulate salts and other solutes C a high density of stomata that are open most of the time D leaves that have a large surface area and a thick cuticle
Mark scheme: B
Q29 · A number of different tissues occur in the walls of major blood vessels
29 A number of different tissues occur in the walls of major blood vessels. Which row correctly identifies the main tissues found in the three layers of the wall of an artery? outer layer middle layer inner layer (tunica externa) (tunica media) (tunica intima) A collagen elastic endothelium B collagen muscle elastic C elastic collagen endothelium D elastic collagen muscle
Mark scheme: A
Q30 · The diagram shows the pressure changes in various structures of the right side of the…
30 The diagram shows the pressure changes in various structures of the right side of the heart during the cardiac cycle. key pressure / kPa X Y Z 0 0 0.2 0.4 0.6 0.8 time / s Which structures are represented by the letters X, Y and Z? X Y Z A pulmonary artery right atrium right ventricle B right atrium pulmonary artery right ventricle C right ventricle pulmonary artery right atrium D right ventricle right atrium pulmonary artery
Mark scheme: C
Q31 · Which statement is true for the Bohr effect?
31 Which statement is true for the Bohr effect? A As the partial pressure of carbon dioxide increases the dissociation curve becomes S- shaped (sigmoid). B At higher partial pressures of carbon dioxide and equal partial pressures of oxygen, the percentage saturation of haemoglobin with oxygen will be higher. C The carbon dioxide in the air prevents haemoglobin becoming fully saturated with oxygen during gas exchange in the lungs. D The release of oxygen from haemoglobin is more likely at higher partial pressures of carbon dioxide.
Mark scheme: D
Q32 · Aortic stenosis is a heart valve disorder in which the aortic semi-lunar valve opening is…
32 Aortic stenosis is a heart valve disorder in which the aortic semi-lunar valve opening is narrow. Which effect could aortic stenosis have on heart structure and function? A The cardiac muscle of the left ventricle wall is thinned by blood leaking out of the left ventricle during ventricular diastole. B There is less cardiac muscle in the left ventricle and reduced diastolic blood pressure, caused by the smaller blood volume entering the left atrium. C The tendons of the heart valves are weakened by blood being forced back through the left atrio-ventricular (bicuspid) valve into the left atrium. D The wall of the left ventricle thickens, leading to an enlarged heart and inability to relax and fill completely during diastole.
Mark scheme: D
Q33 · What can be observed about some of the tissues in a transverse section of a mammalian…
33 What can be observed about some of the tissues in a transverse section of a mammalian trachea? epithelium goblet cells cartilage A ciliated present in blocks B ciliated present in C-shaped rings C non-ciliated absent in blocks D non-ciliated absent in C-shaped rings
Mark scheme: B
Q34 · There is a lot of epidemiological evidence linking smoking to disease and early death
34 There is a lot of epidemiological evidence linking smoking to disease and early death. Which is the best description of ‘epidemiological’? A Disease and deaths are not randomly distributed in small populations. B Disease and deaths are not randomly distributed in the whole population. C Disease and deaths are only randomly distributed in large populations. D Disease and deaths are randomly distributed in the whole population.
Mark scheme: B
Q35 · Which row shows the effects of chronic bronchitis?
35 Which row shows the effects of chronic bronchitis? lymph glands alveoli bronchi infection A destroyed damaged inflamed absent B destroyed inflamed scarred absent C swollen damaged scarred present D swollen inflamed inflamed present
Mark scheme: C
Q36 · A country had fewer than 2.5 deaths per 100 000 people from TB in one year and the next…
36 A country had fewer than 2.5 deaths per 100 000 people from TB in one year and the next year this rose to 25 deaths per 100 000. What may have contributed to this change? 1 decrease in contact tracing 2 increase in refugee camps 3 water supply contaminated by sewage A 1 and 2 only B 1 and 3 only C 2 and 3 only D 1, 2 and 3
Mark scheme: A
Q37 · A graft of tissue, such as skin, from a different person is usually rejected by the body
37 A graft of tissue, such as skin, from a different person is usually rejected by the body. Which statement about graft rejection is correct? A The graft is rejected by B-lymphocytes because they make and release antibodies which react with the surface antigens on the graft cells. B The graft is rejected by B-lymphocytes because T-lymphocytes are not stimulated to produce antibodies. C The graft is rejected by T-lymphocytes because the graft tissue causes T-lymphocytes to release antibodies. D The graft is rejected by T-lymphocytes because they circulate in the blood and can gather at the graft site.
Mark scheme: D
Q38 · The diagram shows a simplified nitrogen cycle
38 The diagram shows a simplified nitrogen cycle. nitrate P legume R Q ammonia S nitrogen nitrogen in primary consumers Which row shows the correct labels for P, Q, R and S? P Q R S A denitrification nitrogen decay of leaf ammonification by anaerobic fixation by tissue by by saprotrophic bacteria nitrifying saprotrophic fungi bacteria bacteria B lightning nitrogen decomposition decomposition action on soil fixation by using by root nodule nitrates nitrogen fixing nitrogenase bacteria bacteria enzyme C nitrification nitrification decay of leaf assimilation of by anaerobic using tissue by organic bacteria nitrogenase saprotrophic nitrogen enzyme fungi D reduction by nitrogen decomposition decay of urea anaerobic fixation by of organic by saprotrophic bacteria root nodule nitrogen bacteria bacteria
Mark scheme: D
Q39 · Which statements about energy flow in ecosystems are correct?
39 Which statements about energy flow in ecosystems are correct? 1 All energy eventually leaves ecosystems in the form of heat. 2 The average energy transfer between trophic levels is 10 %. 3 The energy stored and lost from an ecosystem is equal to the energy input from the Sun. A 1, 2 and 3 B 1 and 2 only C 1 and 3 only D 2 and 3 only
Mark scheme: A
Q40 · In many freshwater ecosystems, the availability of inorganic nitrogen compounds is a…
40 In many freshwater ecosystems, the availability of inorganic nitrogen compounds is a limiting factor for growth. This means that producers cannot grow as quickly as they could even though no other factor is limiting. Which statements about these ecosystems are correct? 1 Transfer of energy to higher trophic levels is also limited by availability of these nitrogen compounds. 2 Addition of excess nitrate compounds will benefit all organisms in the ecosystem. 3 The percentage of energy lost between trophic levels will be the same whether nitrogen compounds are limiting or not. 4 Addition of ammonium compounds will cause an increase in the numbers of nitrifying bacteria. A 1, 3 and 4 only B 1 and 3 only C 2, 3 and 4 only D 2 and 4 only
Mark scheme: A
What was in this paper
The subtopics covered by these 40 questions, and how many questions each got. Open one in a new tab to see every Cambridge question on it.
3Carbohydrates and lipids3Movement into and out of cells3The microscope in cell studies3Transport mechanisms3Cells as the basic units of living organisms2Factors that affect enzyme action2Infectious diseases2Protein synthesis2Proteins2Replication and division of nuclei and cells2The gas exchange system2The heart2Chromosome behaviour in mitosis1Fluid mosaic membranes1Structure of nucleic acids and replication of DNA1Structure of transport tissues1Testing for biological molecules1The circulatory system1The immune system1Transport of oxygen and carbon dioxide1Water1What you needed in this session
Cambridge’s own grade thresholds for 2013 May/June, Paper 1 · Variant 2. A higher threshold means an easier paper — the bar moves with how the cohort did.