Cambridge IGCSE Sciences - Co-ordinated (Double) 0654 — 2009 May/June Paper 5 · Variant 1

This document consists of 10 printed pages and 2 blank pages. IB09 06_0654_05/4RP © UCLES 2009 [Turn over *3412919672* For Examiner's Use 1 2 3 Total UNIVERSITY OF CAMBRIDGE INTERNATIONAL EXAMINATIONS International General Certificate of Secondary Education CO-ORDINATED SCIENCES 0654/05 Paper 5 Practical Test May/June 2009 2 hours Candidates answer on the Question Paper. Additional Materials: As listed in Instructions to Supervisors READ THESE INSTRUCTIONS FIRST Write your Centre number, candidate number and name on all the work you hand in. Write in dark blue or black pen. You may use a pencil for any diagrams, graphs or rough working. Do not use staples, paper clips, highlighters, glue or correction fluid. DO NOT WRITE IN ANY BARCODES. Answer all questions. Chemistry practical notes for this paper are printed on page 12. At the end of the examination, fasten all your work securely together. The number of marks is given in brackets [ ] at the end of each question or part question. www.XtremePapers.com

2 © UCLES 2009 0654/05/M/J/09 For Examiner's Use 1 Hydrogen peroxide is produced in living cells during chemical reactions. It is poisonous if it is not removed quickly. The enzyme catalase is also found in living cells. It speeds up the breakdown of hydrogen peroxide to produce oxygen gas. The experiment is to compare the activity of catalase in different tissues. You will use tissue A, which is potato, and tissue B. When the tissue is added to hydrogen peroxide solution, the oxygen given out is trapped in a foam as shown in Fig. 1.1. The height of foam, h, shows the activity of the catalase. foam hydrogen peroxide and detergent solution sample A h Fig. 1.1 Take care when using hydrogen peroxide. If it gets onto your skin or in your eyes wash it off immediately with cold water. (a) • Measure 10 cm3 hydrogen peroxide into a large test-tube. • Add 2 drops of detergent. • Prepare 2 cubes of sample A of side 1 cm length. Cut each cube in half so that you have four equal pieces. • Add the pieces to the test-tube and immediately start the timer. • Measure the height, h, of the foam every minute for 5 minutes. • Enter your readings in Fig. 1.2. Repeat the complete procedure with tissue B. height of foam h / mm time / mins tissue A tissue B 0 0 0 1 2 3 4 5 Fig. 1.2 [2] The same experiment was carried out earlier with a sample of an animal’s liver, tissue C. These results are plotted on Fig. 1.3.

3 © UCLES 2009 0654/05/M/J/09 [Turn over For Examiner's Use (b) Using the results for tissue A plot the height of foam, h, against time on Fig. 1.3. Draw a smooth curve through the points and label the line A. On the same grid plot the results for tissue B and label the curve. [4] 100 90 80 70 60 50 40 30 20 10 0 1 2 3 4 5 h / mm time / mins Fig. 1.3 tissue C

4 © UCLES 2009 0654/05/M/J/09 For Examiner's Use (c) (i) In which tissue, A, B or C was the catalase most active? How did you decide? [2] (ii) Suggest how the activity of tissue C helps the animal. [2] (d) Look at the graph for tissue C. By how much did the foam increase (i) between 0 and 2 minutes, mm (ii) between 3 and 5 minutes? mm [2] (e) Suggest a reason for the difference between the values of d(i) and d(ii). [1] (f) How can the experiment be adapted to measure the volume of oxygen gas produced? You may use a diagram to illustrate your answer. [2]

5 0654/05/M/J/09 [Turn over BLANK PAGE Please turn over for Question 2.

6 © UCLES 2009 0654/05/M/J/09 For Examiner's Use 2 You are going to find the internal diameter, d, of the large test-tube provided. h large test-tube 100 50 cm3 (a) • Fill the measuring cylinder with water to the 100 cm3 mark. • Clamp the test-tube vertically. • Add water from the measuring cylinder until the test-tube is about one-fifth full. • Measure to the nearest millimetre the height, h, of the water level above the base of the test-tube. Record it in Fig. 2.1. • Record the reading, V, of the water left in the measuring cylinder. h / mm V / cm3 (100 – V) / cm3 Fig. 2.1 [3] (b) (i) Add another portion (about 10 cm3) of water from the measuring cylinder to the test-tube. Measure and record the new height, h, and the reading, V, of the water left in the measuring cylinder. (ii) Repeat (i) to obtain three further sets of readings of h and V. For the last reading the test-tube should be about three quarters full. Record your readings in Fig. 2.1. (c) Complete Fig. 2.1 by calculating the values of (100 – V) [1]

7 © UCLES 2009 0654/05/M/J/09 [Turn over For Examiner's Use (d) On the grid provided, plot a graph of (100 – V) on the vertical axis against h. Draw the best straight line through your points. [4] (e) Use your graph to find the volume, Vw, of water between the heights h = 30 mm and h = 100 mm. Show on your graph how you did this. Vw = cm3 [2] (f) Calculate the internal diameter, d, of the tube using the equation Vw 0.24 d = d = mm [2]

8 © UCLES 2009 0654/05/M/J/09 For Examiner's Use (g) Describe how you would use a long piece of string and a metre rule to measure, as accurately as possible, the outer diameter of the tube. [3]

9 © UCLES 2009 0654/05/M/J/09 [Turn over For Examiner's Use 3 Divide the solid P into three roughly equal parts. (a) Describe the appearance of solid P. [1] (b) (i) Place a portion of solid P on a piece of porcelain and heat it strongly. Observe and record any change in the space below. Allow the residue to cool. observation [1] (ii) Transfer the residue to a test-tube. Add about 2 cm3 of dilute hydrochloric acid and warm gently. Allow the mixture to cool. Add water to fill the tube. Record all your observations. observations [3] (c) Place a fresh portion of solid P in the large test-tube. Add about 10 cm3 of dilute sulfuric acid. Heat until just boiling and place in the rack to cool. When the mixture is cool enough to handle, filter it and collect the filtrate. (i) Describe the appearance of the residue in the filter paper and the appearance of the filtrate. residue filtrate [2] (ii) Place about 2 cm3 of the cooled filtrate in a test-tube. Add an equal volume of potassium iodide solution. Now add about 5 cm3 of solution X. Record all your observations. observations [2]

10 © UCLES 2009 0654/05/M/J/09 For Examiner's Use (iii) Place another 2 cm3 of the cooled filtrate in a test-tube. Add a small portion of zinc powder. Shake the test-tube and contents and place in the rack. Observe the contents after about two minutes. Record all your observations. observations [2] (d) Place the third portion of solid P in a test-tube. Add about 5 cm3 of dilute nitric acid and warm gently. Record all your observations. observations [3] (e) Name the cation in solid P. name of cation [1]

12 Permission to reproduce items where third-party owned material protected by copyright is included has been sought and cleared where possible. Every reasonable effort has been made by the publisher (UCLES) to trace copyright holders, but if any items requiring clearance have unwittingly been included, the publisher will be pleased to make amends at the earliest possible opportunity. University of Cambridge International Examinations is part of the Cambridge Assessment Group. Cambridge Assessment is the brand name of University of Cambridge Local Examinations Syndicate (UCLES), which is itself a department of the University of Cambridge. © UCLES 2009 0654/05/M/J/09 CHEMISTRY PRACTICAL NOTES Test for anions anion test test result carbonate (CO3 2-) add dilute acid effervescence, carbon dioxide produced chloride (Cl-) [in solution] acidify with dilute nitric acid, then add aqueous silver nitrate white ppt. nitrate (NO3 -) [in solution] add aqueous sodium hydroxide then aluminium foil; warm carefully ammonia produced sulfate (SO4 2-) [in solution] acidify then add aqueous barium chloride or aqueous barium nitrate white ppt. Test for aqueous cations cation effect of aqueous sodium hydroxide effect of aqueous ammonia ammonium (NH4 +) ammonia produced on warming - copper(II) (Cu2+) light blue ppt., insoluble in excess light blue ppt., soluble in excess giving a dark blue solution iron(II) (Fe2+) green ppt., insoluble in excess green ppt., insoluble in excess iron(III) (Fe3+) red-brown ppt., insoluble in excess red-brown ppt., insoluble in excess zinc (Zn2+) white ppt., soluble in excess giving a colourless solution white ppt., soluble in excess giving a colourless solution Test for gases gas test and test results ammonia (NH3) turns damp red litmus paper blue carbon dioxide (CO2) turns limewater milky chlorine (Cl2) bleaches damp litmus paper hydrogen (H2) “pops” with a lighted splint oxygen (O2) relights a glowing splint

UNIVERSITY OF CAMBRIDGE INTERNATIONAL EXAMINATIONS International General Certificate of Secondary Education MARK SCHEME for the May/June 2009 question paper for the guidance of teachers 0654 CO-ORDINATED SCIENCES 0654/05 Paper 5 (Practical), maximum raw mark 45 This mark scheme is published as an aid to teachers and candidates, to indicate the requirements of the examination. It shows the basis on which Examiners were instructed to award marks. It does not indicate the details of the discussions that took place at an Examiners’ meeting before marking began, which would have considered the acceptability of alternative answers. Mark schemes must be read in conjunction with the question papers and the report on the examination. • CIE will not enter into discussions or correspondence in connection with these mark schemes. CIE is publishing the mark schemes for the May/June 2009 question papers for most IGCSE, GCE Advanced Level and Advanced Subsidiary Level syllabuses and some Ordinary Level syllabuses. www.XtremePapers.com

Page 2 Mark Scheme: Teachers’ version Syllabus Paper IGCSE – May/June 2009 0654 05 © UCLES 2009 1 (a) readings reasonable (clearly in mm) and entered correctly in column for tissue A ; readings reasonable and entered correctly in column for tissue B ; value for 5 mins in A must not be greater than 80 mm [2] (b) points plotted accurately for tissue A ; allow 1 error smooth curve drawn through points ; points plotted accurately for tissue B ; allow one error smooth curve drawn through points ; [4] (c) (i) liver/tissue C ; fastest rate of production of oxygen ; OWTTE [2] (ii) animals liver has many chemical reactions/produce more hydrogen peroxide ; therefore it is removed more rapidly/it is removed before poison build up ; [2] (d) (i) 82 mm ; (ii) 8 mm ; [2] (e) hydrogen peroxide is running out/has been converted/concentration is falling ; [1] (f) leave detergent out of reaction tube (1) ; collect gas in a measuring cylinder over water or syringe (1) ; [2] [Total: 15] 2 (a), (b) (i) (ii) Table 5 sets for h and V ; h clearly in mm 1st value of h is between 20–40 mm ; last value of h is between 100–140 mm ; [3] (c) 100 – V is correct ; do not award if V is incorrect [1] (d) axes correctly labeled ; scale ; plotting ; allow one error line ; ignore use of origin [4] (e) show how found ; allow if both figures are written down and correct correct evaluation on candidate’s figures ; [2]

Page 3 Mark Scheme: Teachers’ version Syllabus Paper IGCSE – May/June 2009 0654 05 © UCLES 2009 (f) correctly evaluated to at least 1 dp but no more than 2 dp ; accuracy on Vw corrected if necessary ; value between 20–23 [2] (g) wind string around tube & measure ; winding several times ; circumference to diameter ; [3] [Total: 15] 3 (a) red, brown or similar ; allow black if recognizes some brown particles [1] (b) (i) black residue ; if (a) is black then allow black or no change [1] (ii) dissolves ; green solution ; turns blue ; [3] (c) (i) red brown ; filtrate is blue ; [2] (ii) khaki colour (colour similar to khaki) accept yellow/green etc ; white or grey ppt. ; milky is allowed [2] (iii) brown/black solid ; colour fades to colourless or lighter blue, allow ‘fizzing’ ; [2] (d) brown fumes or notes brown solid ; dissolves ; blue solution ; [3] (e) copper (do not allow 2+ oxidation state) do not allow any copper compound ; [1] [Total: 15]